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Research Detail

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Md. Rezaul Karim
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh, Bangladesh

Md. Tariqul Islam
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh, Bangladesh

Khanam Taslima
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh

M Kaniz Fatema
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh, Bangladesh

Harunur Rashid
Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh, Bangladesh

An experiment on the ontogenic development of climbing perch, Anabas testudineus was studied under laboratory conditions at Field Laboratory Complex and Wet Laboratory of the Dept. of Fisheries Management, Bangladesh Agricultural University. The sperms and eggs were collected from males and females by administration of pituitary gland extracts at the doses of 2 and 6 mg/kg body weight respectively. The fertilized eggs were adhesive, transparent and floating with a diameter ranged of 0.7±0.01mm and were incubated in hatching tray. After 15min of fertilization, first cleavage was observed at the temperature ranged of 28±1°C. Hatching started 18.5hr post-fertilization and continued for 2hr at the same temperature. The newly hatched larvae were slender, straight and semi-transperent with an average length of 1.9mm. Larvae started first feeding 48-60hr post-hatching. When the larvae was 5.5mm, yolk sac was absorbed completely. Thus the findings provide valuable information for establishing the culture techniques of A. testudineus.
  Ontogenic Development, Climbing Perch, Anabas testudineus
  Bangladesh Agricultural University, Mymensingh
  00-00-2011
  00-00-2011
  Variety and Species
  Koi/Climbing

To investigate and to provide detailed information of various stages of embryonic and larval development  A. testudineus.

Healthy broods were collected from the nature and stocked in the earthen pond fed with high protein content (35%) artificial feed at the rate of 4-5% body weight. During breeding season both male and female fishes were induced to breed at the PG dose of 2 and 6 mg/kg body weight respectively. Eggs and sperms from female and male were stripped upon ovulation into plastic bowl and mixed thoroughly by using clean and soft feather. The fertilized eggs were incubated in the hatching tray at 28±1°C temperature with continuous water supply. The fertilized egg samples were collected randomly from the hatching tray to study the embryonic developmental stages of A. testudineus at every 15 minutes, 30 minutes and 1 hour interval till completion of morula, gastrula and hatching stages respectively. The egg samples were taken in absolute distilled water for immediate study and in 5% formalin for further study. At least 10 eggs undergoing embryonic developmental process were observed to obtain precise information about developmental stages. In case of larval development study, at least five larval samples were collected from the hatching tank. Initially samples were collected at every four hour and then only once a day until metamorphosis. The early developmental stages were observed by digital microscope (Olympus CX 41) and their image was taken by using Camera (Magnus analytics, Model-MIPS) fixed on a digital microscope. The diameter of the eggs was measured by using an eye piece micrometer and graph paper was used to measure the length of the larvae.
  Proceedings of 3rd International Conference on Environmental Aspects of Bangladesh [ICEAB 2012]; October 13~14, 63-66, 2012
  
Funding Source:
  
The fertilized eggs of A. testudineus were adhesive, transparent and floating with a diameter ranged of 0.7±0.01mm and were incubated in hatching tray. After 15 min of fertilization, first cleavage was observed at the temperature ranged of 28±1°C. Hatching started 18.5hr post-fertilization and continued for 2hr at the same temperature. The newly hatched larvae were slender, straight and semi-transperent with an average length of 1.9mm. Larvae started first feeding 48-60 hr post-hatching. When the larvae was 5.5mm, yolk sac was absorbed completely. Thus the findings provide valuable information for establishing the culture techniques of A. testudineus.
  Report/Proceedings
  


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