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Determination of Protein, Lipid and Carbohydrate Contents of Conventional and Non-conventional Feed Items Used in Carp Polyculture Pond

Asadujjaman M
Department of Fisheries, University of Rajshahi, Rajshahi, Bangladesh

Jahangir Biswas
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh

Manirujjaman M
Department of Biochemistry, Gonoshasthaya Samajvittik Medical College and Hospital, Savar, Dhaka-1344, Bangladesh

Matiar Rahman
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh

Hossain MA
Department of Fisheries, University of Rajshahi, Rajshahi, Bangladesh

Islam MA
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh

Abstract/ Executive Summary:

A study was conducted during April’ 2010-September’ 2010 with a view to compare the protein, lipid and carbohydrate contents in conventional and non-conventional feed items and to recommend suitable strategy in selecting feed item for the development of weed based fish farming in carp polyculture pond. The experiment was carried out at the Protein and Enzyme Research Laboratory, Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi. Six different conventional and non-conventional fish feed items like rice bran, wheat bran, mustard oilcake, Azolla, grass and banana leaves were tested to determine the nutrient contents under 6 treatments as T₁, T₂, T₃, T₄, T₅ and T₆, respectively. In this study, nutrient contents (protein, lipid and carbohydrate) were monitored monthly. Significant variations (P<0.05) were found in the mean values of nutrient contents with different treatments of feed items but in case of same feed item no significant difference was found in the nutrient content at different months. Among the non-conventional feed items treatment T₄ (Azolla) varied more significantly (P<0.05) for the mean values of protein content. Findings indicated that Azolla was more nutritive and low cost effective diets for fish farming in Bangladesh.

Keywords: Azolla, Conventional and non-conventional feed; Carp polyculture;

Location: Kushtia district.

Start Date: 00-04-2010

End Date: 00-09-2010

Program Area: Animal Health and Management

Commodity: Feed

Objectives:

To compare the protein, lipid and carbohydrate contents in conventional and non-conventional feed items and to recommend suitable strategy in selecting feed item for the development of weed based fish farming in carp polyculture pond.

Methodology:

Duration and location of the study: The study was conducted for a period of six months from April 2010 to September 2010. Feed items were collected from the fish farming study site located at Alampur village under Kushtia district of Bangladesh. Whereas nutrient analysis was done at the Protein and Enzyme Research Laboratory under the Department of Bio-Chemistry and Molecular Biology, Rajshahi University, Rajshahi, Bangladesh. Experimental design: The current experiment was carried out under six treatments of feed items each with three replications. The treatment assignments were designated as T₁, T₂, T₃, T₄, T₅ and T₆ for rice bran, wheat bran, mustard oilcake, grass and banana leaves, respectively. Conventional feed items (rice bran, wheat bran, mustard oilcake) were collected from local market during the experimental period. Non-conventional feed item like was collected from ponds adjacent to the research area whereas grass and banana leaf were collected from adjacent grass field and banana garden. Both conventional and non-conventional feed items were collected once a month for nutritional analysis during the experimental period. Nutrient analysis of the collected samples: Total protein, total lipid and total carbohydrate of the collected samples were determined by the micro-kjeldahl method and Anthrone method respectively. Statistical analysis: All the data were subjected to ANOVA (analysis of Variance) using computer software SPSS (Statistical Package of Social Science). The mean values were also compared to see the significant difference from the DMRT (Duncan Multiple range Test).

Source of Information: Fisheries and Aquaculture Journal Volume 2014, 5 • Issue 4 • 1-5 ISSN: 2150-3508

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In case of conventional feed items, protein, lipid and carbohydrate varied from 14.40 ± 0.32% to 30.53 ± 0.40%, 6.69 ± 0.30% to 13.33 ± 0.10% and 32.95 ± 0.29% to 66.12 ± 0.47%. In case of non-conventional feed items, protein, lipid and carbohydrate varied from 6.18 ± 0.13% to 18.58 ± 0.09%, 3.06 ± 0.09% to 6.31 ± 0.13% and 46.36 ± 0.16% to 50.21 ± 0.54%. Inspite of variations weeds are moderately nutritive and low cost effective diets for fish. However, the present study did not evaluate the fish production and economics of feed and weed based systems.

Knowledge Management: Journal