Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effects of Feeding Bacillus Subtilisto Heat Stressed Broiler Chickens with or Without an Antibiotic Growth Promoter

Bibek Chandra Roy
Department of Poultry Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Sachchidananda Das Chowdhury
Department of Poultry Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

S. M. Lutful Kabir
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

The effects of feeding probiotic (PB) with or without Lincomycin 2.2% (AGP) were investigated in broiler chickens during summer. Seven hundred Cobb-500 one day old straight run broiler chicks were randomly distributed into four dietary treatments each of five replications. Four diets were compared: control; control diet plus PB (Bacillus subtilis DSM17299) at a level of 50g/100kg; control plus (AGP) at a level of 15g/100kg and control plus a combination of PB and AGP (50gm/100kg and 15g/100kg respectively). Control birds were fed on a corn-soybean based starter mash that contained ME 3000 kcal/kg and CP 24.39% from 0-21 days of age and a corn-soybean based grower mash of ME 3100 kcal/kg and CP 20.43% during 22-35 days of age. The records were kept of feed intake (FI), body weight and mortality while weight gain, feed conversion ratio (FCR) and survivability were calculated. Temperature and humidity were recorded four times daily except during extreme heat stress when measurements were made more frequently. One bird that was close to average of pen weight was taken from each replication at the end of the trial and the birds were sacrificed to determine carcass characteristics. Both performance and carcass yield data were statistically analyzed. Birds that received PB and a combination of PB +AGP treatments showed significant differences (P<0.01) from control with respect to body weight gain and FCR while feeding AGP alone although showed a decrease in feed intake. Abdominal fat was significantly (P<0.01) reduced in PB treated birds. Higher mortality was encountered in control group. Both PB alone and a combination of AGP treatments were able to show improved performance and reduced mortality, feeding PB alone may be practiced on the combined grounds of improved performance, efficiency in combating heat stress and to discourage the use of AGP in broiler diet.

Keywords: Heat stress, Probiotic, AGP, Broiler chicken, Performance, Carcass traits

Location: Bangladesh Agricultural University Poultry Farm, Mymensingh.

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Fodder

Objectives:

1) To investigate the effects of Bacillus subtilis containing PB on heat stressed commercial broilers when supplemented alone or in combination with AGP

2) To determine the carcass characteristics of finished broilers receiving different treatments

3) To determine the cost-effectiveness of the application of PB and AGP in broiler

Methodology:

The experiment was conducted in the experimental shed of an open sided house at Bangladesh Agricultural University (BAU) Poultry Farm, Mymensingh. Seven hundred Cobb- 500 one day old straight run broiler chicks were considered for the research. The duration of the trial was 35 days. Before the arrival of the experimental birds, the house was properly cleaned, washed, disinfected and dried. Twenty pens of equal sizes were made by wire-net and bamboo materials. The floor space allowed for each bird 1 sq.ft to ensure comfort of the birds. Feeders, waterers, buckets and all other necessary equipments were properly cleaned, washed and disinfected thoroughly with a disinfectant TH₄+ solution (Manufactured by-Sogeval, France, Marketed by-Century Agro Ltd, Bangladesh). Fresh and dry rice husks were used as litter materials at a depth of about 4cm. After 21days, old litter was replaced by fresh rice husk. The experimental broiler chicks were divided into four dietary groups and each group was replicated to five subgroups. First group of chicks was maintained on a control diet, the second group of chicks received an antibiotic growth promoter (AGP, 15g/100kg feed, the third group of chicks was fed a probiotic (Bacillus subtilis strain, 50g/100kg feed and the fourth group of chicks received both AGP (15g/100kg) and probiotic (50g/100kg).

Following arrival, they were placed randomly in the pens and their initial body weights were recorded. They were provided with glucovet powder (a mixture of 490g glucose and 10g vitamin C in 500g powder, manufactured by the ACME Laboratories Ltd, Bangladesh) to overcome dehydration. The temperature and humidity was measured by an automatic digital thermo-hygrometer. Completely vegetable based diets were formulated by using locally available raw materials. Chemical analyses of experimental ration were done in Degussa Lab, Germany (Courtesy of Evonik Degussa GmbH). Birds were supplied starter diet for the first 3 weeks (0-21days) and grower diet for the remaining two weeks (22-35days). Feeds and fresh clean drinking water were offered ad libitum. Strict bio-security program was maintained inside and outside of the experimental sheds as a most effective part of the disease prevention program.

The broiler chicks were weighed group-wise at the beginning of the experiment and then every seven days intervals until the termination of the experiment at 35 days of age. The birds were weighed in the evening between 6.30 and 7.00 PM. Nine chicks from each pen (26%) were randomly weighed. The average live weight was recorded on weekly basis and at the end of the experiment. During the experimental period, usually, the temperature at chick level and that of the house were recorded four times in a day. The relative humidity was also recorded simultaneously by using an automatic thermo-hygrometer. At the end of the trial, the final body weight of the birds was recorded and average body weight was calculated. One broiler having near to pen average weight was sacrificed from each pen for recording meat yield parameters. Data of body weight, body weight gain, feed consumption, feed conversion ratio (FCR), livability and edible meat characteristics were subjected to analysis of variance (ANOVA) in a completely randomized design (CRD) employing SAS (2008) statistical computer package programme.

Source of Information: Asian J. Med. Biol. Res. 2015, 1 (1), 80-88, ISSN 2411-4472

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The application of PB alone in the diet of broiler chicken was found to exert its effects either similar to or better than PB + AGP in combination while better than AGP alone. It appears that PB may be a suitable alternative to AGP used in this study. It is, therefore, concluded that Bacillus subtilis containing PB may be considered as an alternative to AGP on the combined grounds of its performance-enhancing ability, efficiency in combating heat stress and cost-effectiveness.

Knowledge Management: Journal