Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Evaluation of Some Indigenous Plant Extracts for Suppressing Bipolaris Sorokiniana in Wheat Seed

Rokshana Panna
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

F.M. Aminuzzaman
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

M. R. Islam
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

M. H. M. Borhannuddin Bhuyan
Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

Abstract/ Executive Summary:

Studies were conducted in the Seed Pathology Laboratory, Department of Plant Pathology, Sher-e- Bangl a Agricultural University to evaluate some indigenous plant extracts on the incidence of Bipolaris sorokiniana of wheat and seed germination. Seed treated with plant extracts reduced the incidence of Bipolaris sorokiniana. The lowest incidence was counted (12.33%) in seeds treated with Burmuda grass extracts (I :2 ^w/v). While the highest seed germination (99.60%) was recorded in seeds treated with this grass extract followed by 99.33% germination in seeds treated with Neem leaf extract (I :2 ^w/v), 98.00% with nut sedge grass rhizome extract (1:2 ^w/v), 87.67% with Allamanda leaf extract (I :2^w/v). Comparatively the lowest incidence of Bipolaris sorokiniana (14.00%) was observed in seeds treated with Neem leaf extracts (I :2^w/v) followed by 14.50% in seeds treated with Nut sedge grass rhizome extracts (I :2^w/v), Black cumin seed extracts (15.20%) and Allamanda leaf extract (24.00%).

Keywords: Seed sorting, Plant extracts, Black point, Bipolaris sorokiniana, Wheat

Location: Seed Pathology Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

Start Date: 00-01-2007

End Date: 00-09-2007

Program Area: Seed Technology

Commodity: Wheat

Objectives:

To evaluate some indigenous plant extracts for suppressing Bipolaris sorokiniana of wheat and to observe the success in seed germination.

Methodology:

The experiment was conducted at the Seed Pathology Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh from January to September, 2007. The experiment was laid out in Completely Randomized Design (CRD). Four hundred (400) seeds were tested for each treatment. The seed health testing was done by Blotter method. Data was recorded on germination percentage and percent seed affected by Bipolaris sorokiniana. Five different plant extracts of Allamanda leaf, Neem leaf, Nut sedge grass Rhizome, Burmuda grass and Black cumin seed were tested against Bipolaris sorokiniana through five sets of experiments. About 6 kg seed sample of wheat cv. Kanchan was collect from a farmer of Gazipur district and used in this study. Apparently healthy looking seeds were separated from farmer's seed by manual seed sorting by eliminating inert matter, varietal mixture, seeds of other crops and weed seeds, crop residues and black pointed seeds. Botanicals were collected locally and their extracts were prepared by using the method. For preparation of extracts, collected leaves, seeds and rhizomes were weighed and then washed in water. After washing the big leaves and rhizomes were cut into pieces. For getting extract, weighed plant parts were blended in an electric blender and then distilled water was added into the jug of the blender. For getting 1:2 (^w/v) ratios; 200 ml of distilled water was added in 100 g plant parts. Similarly to get 1:4 (^w/v), 1:6 (^w/v) ratios and 1:8 (^w/v) ratios, 400, 600 and 800 ml distilled water was added in 100g plant parts respectively. Desired concentration of the botanicals used In the present experiment. Seeds were treated by dipping method separately in different extracts. Four hundred (400) seeds were dipped in the solution for I hour. The treated seeds were then taken out of the extract and spread on blotting paper to remove excess moisture prior to placing them in the petridishes. For seed health study, three layers of filter paper soaked in sterilized water and was placed at the base of a plastic petridishes (9 cm diameter.) then 25 seeds were placed on filter paper maintaining equal distance among seeds and covered with the lid. The petridishes containing seeds were incubated at 25± I °C for 7 days maintaining alternative 12: 12 hr light and dark cycle in the laboratory. Time to time water was added to keep the filter paper moist. After 7 days of incubation the incubated seeds were examined under stereoscopic microscope in order to record the incidence of Bipolaris sorokiniana. Germination of the seeds was also recorded. Identification of Bipolaris sorokiniana under the stereoscopic microscope was confirmed by preparing temporary slides and examining under the compound microscope with the help of keys described in taxonomic books. Later on the pathogen was purified using hyphal tip culture method and grown on PDA media at 25± I °C for 2 weeks and identified. The collected data on different parameters were analyzed statistically by using MSTAT-C package program. The means for all the treatments were compared by DMRT. The significance of differences among the means was calculated by LSD test.

Source of Information: The Agriculturists 7(1&2).96-104 (2009), ISSN-I729-5211

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

On the basis of the present findings a comparative evaluation of the observed best concentrations of the five plant extracts may be undertaken for final recommendation.

Knowledge Management: Journal