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Effect of Chemical Treatment at Different Moisture Contents on Rice Seed Quality

M. A. Ali
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh

M. M. R. Khan
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh

Abstract/ Executive Summary:

Farmers’ rice seed samples at seven different moisture contents (12-18%) were treated with Provax @ 0.2% to assess its effect on seed health and germination. Treated and untreated seeds were subjected to health and germination tests after four months of storage. Six seed-borne fungi viz. Bipolaris oryzae, Fusarium oxysporum, F. moniliforme, Penicillium sp., Aspergillus sp. and Alternaria padwickii were detected. Incidence of B. oryzae was found highest in all the samples even on treated seeds. Germination failure and seed-borne fungal infection increased with the increase in seed moisture content. Deterioration in germination capacity was more rapid in treated seeds than that of untreated ones. Seed treatment at higher moisture content was detrimental to germination. Chemical treatment at moisture content higher than 12% was harmful for seed germination even though it reduced seed-borne fungal infection.

Keywords: Seed treatment, Germination, Seed-borne fungi, Rice.

Location: Bangladesh Agricultural University, Mymensingh, Bangladesh

Start Date:

End Date:

Program Area: Seed Technology

Commodity: Rice

Objectives:

To observe how different levels of seed moisture in storage affect the germination status and health of rice stored at different moisture levels, and
To evaluate the effect of Provax at different seed moisture content on the aforesaid quality attributes.

Methodology:

Seed samples of BRRI dhan29 at different moisture content were collected from farmers’ fields. Seed moisture content of each of the seed samples was measured by oven dry method. The weight of dish with lid was taken. Five grams of seeds from each sample was taken in a dish, the lid was placed on its mouth and dried at 130⁰C in an electric oven for three hours. After drying the weight (seeds, dish, and lid) was taken. The moisture content (MC) was determined by the following formula: Moisture content (%) = (W₁-W) / (W₁-W₂) x 100. Where, W = Weight of dish with lid; W₁ = Weight of seed, dish & lid before drying; W₂ = Weight of seed, dish & lid after drying. The seed samples having 12, 13, 14, 15, 16, 17, and 18% MC were selected for the study. Moisture content of the seeds were considered as treatments of the experiment; laid out in CRD with four replications. About 500 grams of seeds were used for each replication. The seeds of each replication were taken a polythene bag. One gram of Provax was added in each bag, mixed well, the mouth of each bag was closed and tied firmly. Another set of seed samples having the similar MC as mentioned above were stored without treatment. The bags were preserved in the room under natural conditions. After four months of storage germination (%) and seed-borne infection was determined. Sand was used as medium in plastic trays for germination test. Trays were filled with moist sand. Four hundred seeds were taken at random from each bag. Seeds were sown in sands @ 100 seeds/tray as one replication. Four trays were used for each treatment. After 14 days of sowing the number of normal & abnormal seedlings; dead & non-germinated seeds were counted. Germination was calculated by the following formula: Germination (%) = Number of normal seedlings / Number of seeds tested x 100. Blotter incubation method (ISTA 2003) was employed for seed health testing. Three folds of moist blotter paper were placed at the bottom of a plastic petri-dish. Four plates were used as one replication for each treatment. Twenty five seeds were placed in each plate. The seeds in the plates were incubated at 22 ± 2⁰C under alternating cycle of 12 hours of darkness and 12 hours NUV (near ultra-violet) light for seven days. The seeds were examined under stereo-microscope. The seed-borne fungi on each seed were identified. Seed-borne infection was calculated as percentage of seed infection.

Source of Information: Bangladesh J. Seed Sci. & Tech. 15 (1 &2): 181-185 (2011); ISSN 1029 - 8800

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Six seed-borne fungi viz. Bipolaris oryzae, Fusarium oxysporum, F. moniliforme, Penicillium sp., Aspergillus sp. and Alternaria padwickii were detected. Incidence of B. oryzae was found highest in all the samples even on treated seeds. Germination failure and seed-borne fungal infection increased with the increase in seed moisture content. Deterioration in germination capacity was more rapid in treated seeds than that of untreated ones. Seed treatment at higher moisture content was detrimental to germination. Therefore, it is concluded that chemical treatment of seeds at moisture content higher than 12% is harmful for seed quality even though it reduces seed-borne fungal infection.

Knowledge Management: Journal