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Elisa Based Anthrax Antibody Titer in Cattle Induced by Locally Prepared Anthrax Vaccine Originated from Sterne F-24 Strain in Bangladesh

Jayedul Hassan
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh.

Md. Bahanur Rahman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh.

Shah Md. Ziqrul Haq Chowdhury
Bangladesh Agricultural Research Council (BARC), Dhaka, Bangladesh

Shushanto Kumar Rabidas
Lal Teer Livestock Research and Development Farm, Bongao, Uthura, Valuka, Mymensingh

Md. Shafiullah Parvej
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh.

KHM Nazmul Hussain Nazir
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

Vaccination is usually practiced to prevent and control anthrax in Bangladesh. For this purpose, vaccine prepared from Sterne F-24 strain ofBacillus anthracisby Livestock Research Institute (LRI), Mohakhali, Dhakahas long been used in this country. However, in some cases anthrax occurred in vaccinated animals in Bangladesh. A total of 100 cattle at LalTeer Livestock Research and Development Farm, LalTeerLivestock Limited, Bangladesh, aging between 3-6 years and weighing between 250-400 kg were randomly selected for vaccination purpose. Blood samples (n=100) were collected before the vaccination for collecting pre-vaccination serum, andthe animals were vaccinated (at 1 mL/animal; 1x107 spores/mL) with the anthrax vaccine produced by LRI. All blood samples from the vaccinated animals were collected on day 7, 28, 60, 90, 120, 150, 180, 240, 270, 300, 330, and 360 of post-vaccination, and serum samples were prepared. The antibody levels in the serum samples against anthrax were monitored using an Enzyme-Linked Immunosorbent Assay (ELISA). Over the course of 12 months, the antibody titers were found at the level higher than the reference value. Though there were reports on anthrax suspected cases in this farm, no such cases were reported during the study period. Thus, the vaccine appears to induce adequate antibody response against anthrax in Bangladesh.

Keywords: Cattle, ELISA, Antibody titer, Vaccine, Sterne F-24 strain, Bangladesh

Location: Mymensingh disrict, Bangladesh

Start Date: 00-04-2013

End Date: 00-04-2014

Program Area: Animal Health and Management

Commodity: Cattle

Objectives:

To determine the ELISA Based Anthrax Antibody Titer in Cattle Induced by Locally Prepared Anthrax Vaccine Originated from Sterne F-24 Strain in Bangladesh

Methodology:

The study was performed in a commercial animal facility at Lal Teer Livestock Research and Development Farm, Lal Teer Livestock Limited, Bangladesh, located at Bongao, Uthura, Valuka, Mymensingh, during the period from April 2013 to April 2014. The anthrax vaccine was collected from the Livestock Research Institute (LRI), Mohakhali, Dhaka, Bangladesh. The master seed of this vaccine contained living spore of the non-capsulated attenuated Sterne F-24 strain of B. anthracis obtained originally from Australia. A total of 100 healthy and unvaccinated cattle at the farm were randomly selected for this study. The cattle received anthrax vaccine in previous years as a routine work. However, the cattle did not receive any anthrax vaccine within past one year when the study started. The cattle were aged between 3-6 years weighing between 250-400 kg. For the determination of antibody level against anthrax, blood samples (10 mL each; n=100) were collected before vaccination of the animals. Then all the cattle were vaccinated following the schedule mentioned by LRI. Briefly, 1 mL vaccine containing 1×107 spores (approximately) was injected per animal through subcutaneous route at neck region. Repeat sets of blood samples (n=100) were collected from the vaccinated animals on day 7, 28, 60, 90, 120, 150, 180, 240, 270, 300, 330, and 360 of post-vaccination. All blood samples were carried in ice box to the Laboratory at the Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202. Serum samples were prepared from the blood samples according to the method. The antibody level in the serum samples were determined by ELISA (AniGenB. anthracisAb ELISA kit, BIONOTE Inc., Republic of Korea).

Source of Information: Microbes and Health, January 2015, 4(1): 36-38.; ISSN: 2226-0 153 (Print) 2305-3542 (Online)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The anthrax vaccine manufactured by LRI, Mohakhali, Dhaka, Bangladesh could protect the vaccinated cattle from anthrax up to one year. Therefore, the anthrax vaccine produced by this institute may be effectively used to prevent anthrax outbreaks in animal population of Bangladesh. However, further study of the immunogenicity of the vaccine along with challenge exposure is recommended to establish whether, the antibody induced by the vaccine is protective or not.

Knowledge Management: Journal