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Growth and Reproductive Performance of Locally Isolated Brackishwater Rotifer (brachionus Plicatilis) Feeding Different Micro Algae

M.J. Alam
Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna 9280, Bangladesh

M.M.R. Shah
Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna 9280, Bangladesh

Abstract/ Executive Summary:

Population growth and reproductive capacity of brackishwater rotifer, ?Brachionus plicatilis, were evaluated, for a period of 8 days in a temperature controlled ( =25°C) microalgal laboratory, under three different algal feeding regimens. The algal species that were tested are: (i) Chlorella sp. (T₁), Tetraselmis chui (T₂), Nannochloropsis oculata (T₃). The feeding density of each algal species was maintained similar as of 4.5x10⁶ cells ml. The rotifer fed on T. chui showed the highest (p<0.05) population growth (131.5 ind./ml), compared to that fed on Chlorella sp (45.67 ind./ml) and N. oculata (43.44 ind./ml). The abundance of egg bearing rotifers was also higher (35.77%) with T. chui than with Chlorella sp. (27.76%) and N. oculara (24.60%). The results of the present study indicate that T. chui could be the most suitable algal food for the stock culture of locally isolated rotifer B. plicatilis.

Keywords: Microalgae, Rotifer, Growth, Reproductive performance, Brackishwater

Location: Bangladesh Fisheries Research Institute (BFRI), Paikgacha, Khulna

Start Date: 00-00-2002

End Date: 00-00-2002

Program Area: Animal Health and Management

Commodity: Aquatic animal

Objectives:

To determine the feeding effect of three different locally available microalgal species on the growth and reproductive performance of locally isolated brackishwater rotifer, Brachionus plicatilis under stock culture condition.

Methodology:

The stock culture of the rotifer was done in a temperature controlled (about 25^oC) ?microalgal laboratory at the Brackishwater Station of Bangladesh Fisheries Research Institute (BFRI), Paikgacha, Khulna. Three different microalgae, viz., Chlorella sp. (T₁), Tetraselmis chui (T₂), and Nannochloropsis oculata (T₃) were used as food to evaluate their effects on growth and reproductive performance. Nine 500 ml Erlenmeyer flasks were divided into three treatment groups. The flasks were placed at a distance of approximately 30 cm from a fluorescent tube light, having an intensity of about 2500 lux/m²/sec. Photoperiod was maintained as 16:8 hrs L:D. Chlorella sp., Tetraselmis chui, and Nannochloropsis oculata were collected, isolated and cultured separately in laboratory conditions using the BFRI microalgae culture medium. When the concentration of each algae reached to 4.5x10⁶ cells/ml, three rotifer culture flasks were filled in up to 300 ml with each algal culture following completely randomized design. Rotifers were collected and isolated from the local brackishwater rivers and shrimp ponds using a zooplankton net of 250 to 90 micron mesh. Each of the test flasks was inoculated with rotifer at a density of 5 ind./ml. The salinity of microalgae and rotifer culture water was 20%o. The initial abundance of initial egg bearing rotifers was estimated to about 12.53%. The duration of algal feeding trial on growth and reproductive capacity on rotifer was 8 days. The concentration of rotifers was estimated every two days. Depending upon rotifer density, an unspecified amount of rotifer culture was poured through a 45 micron sieve to catch a large number of rotifers on the screen. These were then rinsed with a small amount of filtered seawater using a squirt (wash) bottle and placed in to petri dish. These concentrated rotifers were then sampled with a bulbed pasteur pipette and placed on the Sedgewick-Rafter counting cell and counted under the dissecting microscope. The mean count was obtained from 6 counts. Instantaneous growth rate (K) was calculated. While the growth of rotifer was estimated under microscope, the number of egg bearing rotifers was also recorded. The percent abundance of egg bearing rotifers (EBR) was estimated, to understand the reproductive capacity (RC) of rotifers. Data were analyzed following one way ANOVA and Duncan's Multiple Range Test (DMRT), using a PC equipped with STATAGRAPHICS Ver. 7.

Source of Information: Bangladesh J. Fish. Res.,8(2), 2004: 127-133

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

T. chui could be the most suitable algal food for the stock culture of locally isolated rotifer B. plicatilis.

Knowledge Management: Journal