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Establishment of Satellite Nucleus of Genetically Improved Farmed Tilapia (gift Strain) in Bangladesh

A.H.M. Kohinoor
Bangladesh Fisheries Research Institute, Mymensingh 2201, Bangladesh

M.S. Islam
Bangladesh Fisheries Research Institute, Mymensingh 2201, Bangladesh

R.W. Ponzoni
WorldFish Center, ]alan Batu Maung, Batu Maung, 11960 Bayan Lepas, Penang, Malaysia.

N.H. Nguyen
WorldFish Center, ]alan Batu Maung, Batu Maung, 11960 Bayan Lepas, Penang, Malaysia.

S.U. Ahammed
Bangladesh Fisheries Research Institute, Mymensingh 2201, Bangladesh

M.G. Hussain
Bangladesh Fisheries Research Institute, Mymensingh 2201, Bangladesh

Abstract/ Executive Summary:

The aim of this study was to evaluate growth performance of the GIFT (Genetically Improved Farmed Tilapia) strain after one generation of selection for increased body weight at Bangladesh Fisheries Research Institute. Founder stock comprised of 30 families having 300 individuals of the GIFT strain were introduced from Malaysia through WorldFish Center in March 2005. The founder stock was reared in 100 m²hapa for three months and then individually tagged using Passive Integrated Transponder (PIT) at the weight between 30 and 40 g. After tagging, all the fish were communally grown out in pond until harvest. Breeding value for body weight was estimated using SAS and ASREML ranging from 4.17 to 9.70 g for males and 4.24 to 9.36 g for females. The best 40 females and 40 males from the founder stock were then selected to produce progeny of the first generation (F₁). From each family 25 female and 25 male fingerlings were sampled and tagged using PIT. A total of 2,000 tagged fish from 40 families were stocked in a pond (1000 m²) for a continuation of the selection program. In addition, surplus fish after tagging were also reared together with progeny of the founder stock in cistern ecology for growth evaluation. The mean weight of the F₁ generation of GIFT fish was 7.2% greater than that of the founder population (non selected population).

Keywords: Satellite nucleus, Genetically Improved Farmed Tilapia (GIFT), WorldFish

Location: Bangladesh Fisheries Research Institute

Start Date: 00-00-2007

End Date: 00-00-2007

Program Area: Variety and Species

Commodity: Tilapia

Objectives:

To evaluate the growth of F₁ generation of improved GIFT strain in Bangladesh.

Methodology:

Origin of stock: Founder stock comprised of 30 families having 300 individuals of GIFT Strain were introduced from Malaysia through WorldFish Center in March 2005. The founder stocks was reared in 100 m² hapa for three months. The fish were fed with SABINCO feed (28% crude protein) at the rate of 6% of estimated biomass. After three months rearing, the mean weight of female and male were 41.18±5.41 and 30.42±3.47g, respectively. Tagging of founder stock: Then the female and male were tagged by using Passive Integrated Transponder (PIT). A PIT tag was injected into the peritoneal cavity .of a fish and the number of tag was recorded. After tagging all the fish were transferred to a pond having 1000 m² area. Rearing in pond: During rearing period, the fish were fed with supplementary feed six days in a week (28% crude protein) at the rate of 4-5% of estimated biomass. Fish were sampled at fortnightly interval to assess the growth and feed adjustment. Water was supplied once in a week to maintain water depth at 1.0 meter. Pond was fertilized fortnightly with Urea and TSP at the rate of 12.5 and 25.0 kg/ha, respectively. After four months rearing, the fish were recaptured through seine netting and pond drying. The final body weight, sex and tag number of all harvested fish were recorded. Estimation of breeding value: Breeding value was estimated for individual fish in a full pedigree, using SAS and ASREML. Breeding in hapa: On the basis of breeding values of the founder stock, the best 40 males from 30 families were crossed with 40 best females (from 30 families) for the production of F-1 generation. For breeding, 40 breeding hapas (l.0m³) were set up in a pond with bamboo poles. A pair of female and male breeders (1: 1) was stocked in each breeding hapa. After 12 days of stocking, fertilized eggs were collected from brooding females. After that, collected eggs were transferred to the hatchery for incubation. Immediately after hatching, the larvae were shifted to a series of trays and were kept until their yolk sac resorption stage. Nursing in hapa: After resorption stage, 300 fry from each family were transferred to 40 individual fine mesh nursery hapas (2.0 m³) in pond. The progeny were fed with nursery feed containing 30% protein at the rate of 30% of estimated body weight. After 45 days nursing, the mean weight of the fry was 2.80±0.42g. Rearing in hapa: Subsequently, 150 fry from each progeny group were transferred to 40 individual rearing hapa (2.0 m³ in size). Supplementary feed (Nursery feed) was applied in all the hapas at the rate of 15% of estimated biomass. After two months of rearing, the weight range of male and female were 36-43 and 28-32g, respectively. Tagging: From each progeny group 25 male and 25 female fish were selected and tagged using Passive Integrated Transponder (PIT). Tagged fish from 40 families (2000 fishes) were stocked in a pond having 1000m² area for communal rearing. Supplementary feed (25% crude protein) was supplied regularly at the rate of 6% of estimated biomass. After six months of grow-out in pond, the fish were harvested, and tag number, weight, sex, body depth were recorded. After harvesting, breeding values of F-1 generation were estimated from the complete data set, tracing back to the foundation population (F0). Evaluation of Growth performances: This trial vvas conducted to compare growth performance between F₁ progeny of the selected fish (F₁ selected GIFT) and progeny of the non-selected population (founder stock) in cisterns (2.0 m³) for a period of four months during April to July 2007. Progeny of the selected fish were produced from 40 single pair matings in separate hapas. Family rearing of the selection progeny was as described above. After tagging, surplus fish were sampled for this experiment. By contrast, the non-selected population (200 breeders) was stocked in a 300 m² pond for mass breeding. After 40 days of stocking, 6,000 fry were collected and reared in a 10m³ hapa for a period of 3 weeks. From this population, fry samples were taken for growth evaluation. The initial mean weight of the selected fish and of the founder population (non selected population) were 2.95±0.65 and 2.65±0.82g, respectively. There were two treatments with three replicates. Before stocking the cisterns were cleaned and filled up with deep tube well water at the depth of 1.0 meter. Fry of GIFT strain were stocked at a density of 5 fish/m³^_.The fry of both treatments were fed twice a day in six days in a week with supplementary feed (28% crude protein) at 5-8% body weight. During grow out period, first and second months, feed was given at the rate of 8% and 7% of body weight, respectively, Then subsequently, 6% and 5% feed were given to the fish in the 3rd and 4th month, respectively. Fish sampling was done at monthly interval to assess the growth, and feeding ratio was adjusted on the basis of estimated weight of fish biomass. In every week cisterns were cleaned through siphoning and 80% water changed with deep tube well water. Average water depth was maintained in all the cisterns at 1.0 m during the experimental period. After four months rearing, all the fishes were harvested. After harvest, body weight was measured on individual fish. Statistical analysis was carried out to test significant differences in growth between the F₁ generation fish and the founder stock.

Source of Information: Bangladesh J. Fish. Res., 12(1 ), 2008: 35-40

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The mean weight of the F₁ generation of GIFT fish was 7.2% greater than that of the founder population (non selected population).

Knowledge Management: Journal