Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Bacterial Load in Pond Water and Different Organs of an Indian Major Carp Cirrhinus Mrigala Ham.

A.N.H. Banu
Bangladesh Fisheries Research Institute, Mymensingh 2201, Bangladesh

M.A. Islam
Department of Fish. Biology & Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

M. B. R. Chowdhury
Department of Aquaculture, BAU, Mymensingh 2202

Abstract/ Executive Summary:

During the study period (August, 1993 ro July,l994) the mean bacterial load in surface water was found to vary from 1.39 x10⁵ (July'94) ro 3.11 x 10⁷CFU/ml (September'93), while that of botom water ranged from 1.01xl0⁶ (May'94) to 5.90x10⁷CFU/ml (October '93).The mean total number of bacterial load in body slime, liver and kidney was found to vary from 0.58x10³ (July'94) to 2.37x10⁷CFU/g (March'94),from 0.22x10³(July'94) to 9.64xl06 CFU/g (March'94) from 0.15x10³(July'94) to 9.36xl0⁶ CFU/g (March'94), respectively. Bacterial load in slime was significantly correlated with bacterial load in liver, bacterial load in slime was significantly correlated with bacterial load in kidney and bacterial load in liver was significantly correlated with bacterial load in kidney.

Keywords: Bacteria, Mrigal, Pond water, Bacterial load

Location: Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh

Start Date: 00-08-1993

End Date: 00-07-1994

Program Area: Animal Health and Management

Commodity: Carp fish

Objectives:

To investigate the bacterial load in pond water and different organs of the bottom living fish, Cirrhinus mrigala H.

Methodology:

Sampling: Fish samples (Length26.37cm to 35.62cm) of mrigal, Cirrhinus mrigala H. were collected once a month from the selected experimental pond of the Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh for period of 12 months. Water samples was collected also from the same pond once a month during the study period. Water samples were collected in sterilized reagent bottles both from the surface and bottom levels for bacterial population study as well as for studying the physico- chemical parameters. Water study: Surface and bottom water of the pond was carried out followed the pour and spread plate method as stated in Standard Methods for the examination of Water and waste water. Petri dishes were set out, two plates per dilution were tested. Each dilution (0.1 ml) was pipetted into the center of the petri dishes using a fresh pipette for each dilution. With a sterilized glass rod the content was spread as quickly as possible and the medium was allowed to set. Then the petri dishes were inverted and kept in incubator at 25°C for 24-48hr.The plates having 30-300 colonies were counted for viable count. The colony or viable count /ml was calculated by multiplying the average number of colonies per countable plate by the reciprocal of the dilution. Body slime: Fish samples collected for investigation were killed by a light hurt on the neck region and then the slimes were collected by scrapping with a sterile scalpel and were kept in a sterile weighing boat which were pre- weighed. Necessary dilution were made using physiological saline (0.85% NaCl) following the ten fold dilution method, Thus the samples were ready for inoculation in the culture medium.(TSA,Oxoid). Liver: The body surface of fish samples were disinfected with 70% ethyl alcohol and their abdomen opened by aseptic dissection and then the liver was taken out carefully with the help of sterilized forceps. The liver samples after being weighed individually in a sterile weighing boat and was homogenized for preparation of suspension in physiological saline. Thereafter, the suspension was diluted with sterile physiological saline for inoculation on the culture media (TSA,Oxoid). Kidney: The kidney samples were prepared following the same procedure as done for the preparation of liver.

Source of Information: Bangladesh J. Fish. Res., 5(1), 2001: 53-58

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The bacterial population in different substrates were 1.39x 10⁵ to 3.11x 10⁷CFU/ml in surface water 1.01x10⁶ to 5.90x 10⁷ CFU/ml in bottom water, 0.58x10³ to 2.37 x10⁷ CFU/g in body slime, 0.22x10³ to 9.64 x10⁶ CFU/g in liver and 0.15x10³ to 9.36x 10⁶ CFU/g in kidney. The study revealed that there is an existence of positive correlation of bacterial load in body slime with liver as well as with kidney of C. mrigala.

Knowledge Management: Journal