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Pollen Tube Growth in Crosses Between Porteresia Coarctata and Oryza Sativa.

Dr. M A Samad
Chief Scientific Officer
Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus< Mymewnsingh-2202

R.H. Sarker
Professor
Department of Botany, Dhaka University, Bangladesh

Z Seraj
Professor
Department of Biochemitry, Dhaka University, Bangladesh

M I Hoque
Professor
Department of Botany, Dhaka University, Bangladesh

A S Islam
Professor
Department of Botany, Dhaka University, Bangladesh

Abstract/ Executive Summary:

Comparative study with fluorescence microscopy revealed that the pollen tubes of the self-pollinated rice variety BR-9 reached the ovary within 75 min after pollination. In P. coarctata it took 150 min. In the cross between P. coarctata and BR-9; pollen tubes reached the ovary in 165 min but seed setting was not observed. In the cross between BR-9 and P. coarctata, small and deformed pollen tubes were formed and they failed to grow through the stylodium.

Keywords: Porteresia coarctata. Oryza sativa, Rice, intergeneric cross, Fluorescent microscopy, Pollen tube, Callose, Postzygotic barrier

Location: Field Labrotory, Department of Biochemistrey, Dhaka University

Start Date:

End Date: 00-00-1992

Program Area: Variety and Species

Commodity: Rice

Objectives:

If pollen tubes reach the ovule in any of the directions, such a study may guide the breeders as to the direction in which the future crosses should be made

Methodology:

The following materials were used for the investigation. a) Oryza sativa L. var BR-9. an indica HYV obtained through the courtesy of Bangladesh Rice research Institute (BRRI). b) rhizomes of Porteresia coarctata collected near Sonagazi in the coastal belt of Noakhali, Bangladesh. Seeds and rhizomes were grown in the clay pots containing normal soil and maintained in the Department of Biochemistry, Dhaka University during the growing seasons of 1991-1992. The experiment plants were grouped into three sets. In the first and second sets self-and reciprocal cross pollination were carried out respectively. The third set was maintained to observe seed setting following self-and cross pollination. In all cross emasculation was carried out with a pair of fine forceps at least 12 hrs before pollination. Pollination was made by gently touching newly dehiseed anthers to the stigmatic branches. To determine -pollen germination and tube growth, pollinated pistils were collected and fixed in acetic acid: alcohol (1:3, v/v) at various intervals from the time of pollination. At each interval of time, at least 20 pollinated pistils were collected for microscopic observation. The collected pistils were then softened in 1N NaOH at 60⁰C for 10 min and thereafter stained with 0.1% (w/v) decolourized aniline blue solution. The stained materials were observed under a fluorescence microscope (Nikon Microphot, Japan) following the technique of Linskens and Esser (1957). For all experiments pollen germination and tube growth were recorded.

Source of Information: Journal, Euphytica 69: 129-134, 1993

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the cross between BR-9 and P. coarctata, small and deformed pollen tubes were formed and they failed to grow through the stylodium.

Knowledge Management: Journal