10 cm × 20 cm (S4)
Experimental design and layout
The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replications. The treatments were randomly assigned in each replication. There were 36 unit plots in the experiment. The size of each plot was 4 m × 2.5 m. Each unit plot and block was separated from each other by 50 cm and 1m, respectively.
PRE HARVEST STUDIES:
Plant population per m2 after establishment of seedlings, maximum tillering stage and panicle bearing shoots per m2
Plant population just after establishment of seedlings, maximum tillering stage and panicle bearing shoots per m2 were counted at 50 and 80 days after transplanting of crop.
Total productive and unproductive tillers per m2
The number of total tillers per m2 was computed by deducting initial plant population from plant population at maximum tillering stage. The productive tillers per unit area were computed by deducting initial plant population from panicle bearing shoots. The unproductive tillers per unit area were computed by deducting panicle bearing shoots from plant population at maximum tillering stage of the crop.
Plant height
Plant height was recorded at 50 DAT, 80 DAT and harvesting stage of crop in cm with the help of meter scale. For this observation five hills were tagged randomly in each plot for measuring the height. The height of plant measured from the ground level up to the tip of the leaves of fully open leaf.
Lodging percentage
Lodging percentage were determined at milking stage and maturity stage from a square meter of each plot by counting total number of plants minus number plant remain erect, then expressed into percentage.
POST HARVEST STUDIES
All the yield contributing characters were studied at maturity of crop. Five hills per plot were selected randomly for studies yield contributing characters.
Length of panicle
Length of panicle were recorded by measuring all sampled panicle from base of panicle up to the end of terminal rachillae and average was calculated to get the mean length of panicle.
Number of spikelets per panicle
The spikelets received from the sampled panicle were counted separately and their average number was recorded.
Number of grain per panicle
The total number of grains per panicle was counted by the following equation from the sample, which was earlier used for recording the number of grains per panicle and average for statistical analysis.
Total No. of spikelets = No. of grain + No. of sterile spikelets.
1000-grain weight
Cleaned grains sample was taken from each plot and 1000-grain was counted from seed counter for each sample and weighted in gram.
Biological yield
The total produce of net plot was allowed to dry in the field for three days and after drying biological produce tied into bundles and weighed by spring balance in kg and converted into ton per hectare.
Grain yield
The grains obtained from each plot were weighed by pan balance in kg and converted into ton per hectare.
Straw yield
The grain yield was subtracted from the biological yield per plot to record the straw yield kg per plot, which was converted into ton per hectare.
Harvest Index
Harvest index was calculated on the basis of adjusted grain and straw yield using the formula recommended by Gardner et al. 1985.
Harvest index (%) = 
x 100