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Growth Performances of Three Microalgal Species in Filtered Brackishwater with Different Inorganic Media

M.M. R. Shah
Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna 9280, Bangladesh

M.J. Alam
Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna 9280, Bangladesh

M.L. Islam
Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna 9280, Bangladesh

M.S.A. Khan
Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna 9280, Bangladesh

Abstract/ Executive Summary:

The growth of three microalgae species, viz., Nannochloropsis oculata, Tetraselmis chui and Chaetoceros muelleri which are commonly used in aquaculture, was investigated using three different inorganic nutrient media: (i) Modified Guillard's f/2 medium (ii) Rix Mix medium and (iii) BFRI medium. Each microalgae species was cultured for 24 days in small- scale with initial inoculation density of 17xl0⁴ cell /ml in the three media with triplicates. N. oculata cultured in modified Guillard's f/2 medium showed superior growth with a mean peak density of 221 ±4.24 x 10⁴ cell/ ml, to Rix Mix medium (141 ± 10.54xl0⁴ cell/ml) and BFRI medium (47±4.94 x 10⁴ cell/ml) on the 16th day of culture at stationary phase. Considering the increase in cell density for 20 days of culmre in Rix Mix medium, C. muelleri was significantly (P<0.05) highest than in other two media. N. oculata cultured in BFRI medium resulted in the poorest growth with a mean peak increase in density of 84±9.19 x 10⁴ cell/ml in 12 days of culture. However, with an increase in cell density, growth of T. chui (182 ± 6.26 x 10⁴ cell/ml) was significantly (P<0.05) higher in BFRI medium than in modified Guillard's f/2 medium. The results of the present study suggest that N. oculata and C. muelleri can be grown very well in both the modified Guillard's f/2 medium and Rix Mix medium. Bener growth of T. chui can be obtained while culturing either in BFRI and Rix Mix medium. These three nutrient media used in the present study may be useful for microalgae species culture for establishing green-water culture for suitable target zooplankton, and fish and crustacean larvae in marine and brackishwater hatcheries.

Keywords: Microalgae, Brackishwater, Nutrient media, Zooplankton

Location: Brackishwater Station of Bangladesh Fisheries Research Institute (BFRI), Paikgacha, Khulna

Start Date: 00-00-2001

End Date: 00-00-2001

Program Area: Animal Health and Management

Commodity: Aquatic animal

Objectives:

To determine the effect of three selected inorganic nutrient media on the growth performance of above three microalgae.

Methodology:

The culture of the microalgae in these trials was done in a temperature controlled (about 25 °C) microalgal laboratory at the Brackishwater Station of Bangladesh Fisheries Research Institute (BFRI), Paikgacha, Khulna. The culture was maintained in 1.5-liter mineral water bottles with sufficient aeration in axenic condition. The brackishwater of 25 ppt was filtered through 1.0 micro meter cartridge filter to remove particulate and treated with 30 ppm chlorine at the rate of 0.1 g/l and dechlorinated by using 0.175 g/l of sodium thiosulphate. As excess of sodium thiosulphate may reduce trace metal availability, the rate of application and period of dechlorination for 30 minutes were maintained properly. Media types tested were modified Guillard's f/2 medium, Rix Mix medium and BFRI medium. The standard nutrient medium used for algal culture was the Medium-F suitable for the growth of most algae and is being used extensively. The modified Guillard's f/2 medium used in this study was modification from Guillard's F medium for microalgal culture. The Rix Mix medium used by the private laboratory in North Queensland, Australia for stocks and medium size working culture. The BFRI medium was prepared at the laboratory, which contain similar trace metals and vitamin solution of modified Guillard's f/2 medium. The composition of Rix Mix, BFRI medium and modified Guillard's f/2 medium. The application rate of BFRI medium and modified Guillard's f/2 medium was 1 ml/L brackish water and for Rix Mix it was 2 ml/L brackishwater. The application rate of sodium metasilicate was 1 ml to 1-L brackishwater for diatom only. The algal cells were inoculated into the bottles with equal initial concentration (17x10⁴ cell/ml) for all species of microalgae. Aeration was moderate and the source was aquarium aerators. The bottles were set up approximately 15 cm from the two 'daylight' fluorescent tube light sources with a light intensity of about 2500 lux /m²/ s. There were three replications for each treatment i.e. for each nutrient medium. The total culture period was twenty-four days. Counts of algal cells were made at every four days interval using an improved Neubauer Haemocytometer by applying the methodology used by Hoff and Snell. All the data were analyzed statistically using Analysis of Variance (ANOVA) and the significance test among mean values of treatments was done using Duncun's New Multiple Range Test.

Source of Information: Bangladesh J. Fish. Res., 7(1), 2003: 69-76

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

N. oculata and C. muelleri might be grown very well in both the modified Guillard's f/2 and Rix Mix media. Better growth of T. chui might be obtained while growing in BFRI and Rix Mix media. These three microalgal culture media used in the present study may be useful for culture of different algal species to supply as live food for suitable target zooplankton and larvae of fish, crustaceans, etc. in marine and brackishwater hatcheries.

Knowledge Management: Journal