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Proline Content and Nitrate Reductase Activity of Some Groundnut Genotypes as Index of Screening for Drought Tolerance

D. E. Jharna
Dept. of Agril. Chemistry, Patuakhali Sci. & Tech. University, Patuakhali, Bangladesh.

B. L. D. Chowdhury
Department of Biochemistry, Bangladesh Agricultural University. Mymensingh

M. R. H. Bhuiyan
Department of Biochemistry, Bangladesh Agricultural University. Mymensingh

M. A. Haque
BRRI, Regional Station, Barisal, Bangladesh

Abstract/ Executive Summary:

A pot experiment was conducted for screening of some drought tolerant gruundnut (Arachis hypogaeu L.) genotypes. Plant were grown in Hoagland solution and water stress was imposed by adding 4% polyethylene glycol-6000. As index or drought tolerance change in free proline content and stability of nitrate reductase activity in the leaves of water stressed plants were monitored. In the drought tolerant plants the increase in proline over the control varied from 5.29 to 7.93 fold and the decrease in nitrate reductase activity ranged from 0.2 - 4.1%, On the basis of increase in proline accumulation and degree of stability of nitrate reductase. 3 genotypes were selected as drought tolerant and they were M-5. DG-2 and 3203.

Keywords: Proline, Nitrate reductase, Groundnut, Drought

Location: Department of Biochemistry Bangladesh Agricultural University, Mymensingh.

Start Date: 00-08-2000

End Date: 00-10-2000

Program Area: Risk Management in Agriculture

Commodity: Ground nut

Objectives:

To select some drought tolerant groundnut genotypes based on greater proline accumulation and stability of nitrate reductase activity.

Methodology:

For screening of drought tolerant groundnut genotypes a pot experiment was carried out in the laboratory of the Department of Biochemistry Bangladesh Agricultural University (BAU). Mymensingh during August to October. 2000. The plants were grown in hydroponic media using Hoagland nutrient solution. The concentration of different ingredients of Hoagland solution are mentioned. Plants were grown in cylindrical plastic pots of 16 cm high and 9.5 cm diameter. The cap of each pot contained two circular opening of I cm each in diameter. Seven days old seedlings were planted in Hoagland solution. One seedling was planted in one hole of cap of the plastic pot and the other hole was left open for adding nutrient solution and aeration. Seedlings were fixed properly in the hole with the help of a small piece of foam. One litre Hoagland solution was taken in each pot. This volume was maintained by adding required amount of distilled . water everyday. The pots were placed under sunlight. When the plants were four weeks old, water stress was imposed. The water stress condition was developed by adding 4% polyethylene glycol- 6000 (PEG-6000) with the Hoagland solution. The control plants were grown all along in Hoagland solution. The experiment was laid out in factorial combinations of groundnut genotypes and water stress level following completely randomized design (CRD) with three replications. After imposing the water stress, proline content in the leaves of the individual plants was determined at 7^th and 15^th day. Generally. 2^nd or 3^rd descending apical leaves were collected and immediately subjected to analysis. Each analysis was done in duplicate. Proline content of leaves was determined. Nitrate reductase (NR) activity of leaves was determined after 20 days of stress imposition. Data were analysed using ANOV A and DM RT.

Source of Information: Progress. Agric.13(I & 2) :11-14. 2002 ISSN 1017-8139

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the present study, the groundnut genotypes. considered as drought tolerant (M-5. DG-2 and 3203) were also found to retain the nitrate reductase activity in water stress condition. Other genotypes showed higher percentage of loss of NR activity.

Knowledge Management: Journal