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Marker-assisted Foreground Selection for Identification of Salt Tolerant Rice Genotypes

M. S. Alam
Regional Agricultural Research Station, BARI , Akbarpur, Moulvibazar, Bangladesh.

M. Salim
Hill Agricultural Research Station, BARI , Khagrachari, Bangladesh.

M. Moniruzzaman
Bangladesh Jute Research Institute (BJRI), Manikgonj, Dhaka, Bangladesh.

J. A. Rashid
MATEX Bangladesh Ltd. Dhaka, Bangladesh.

M. M. Islam
Biotechnology Div., Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh.

Abstract/ Executive Summary:

Marker Assisted Selection (MAS) technique was used to develop salt tolerant rice genotypes using molecular markers during June 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. FL-378 was identified as donor or male parent for saltol QTL and Binadhan-7 as recurrent or recipient parent which had high yield with short life cycle. Crossing was done between them and 10 F 1 seeds were produced. PCR bands from all the 10 F 1 plants were scored as “H” represented heterozygous alleles for donor and recipient parent. Backcrossing was done to produce 105 BC1F1 seedlings. Foreground selection was performed with 72 BC 1F1 plants with a tightly linked salt tolerance marker RM21. Out of 72 plants, 33 plants were selected for RM21 showing ‘H’ scores. The selected segregants were subjected to further recombinant and background selections at BC 1 F 1 generation. These selected genotypess could be used for further foreground, recombinant and background selections with appropriate markers upto BC 3 generation for the development of salt tolerant rice genotypes.

Keywords: Marker-Assisted Selection (MAS), foreground selection, salinity, SSR marker

Location: Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh.

Start Date: 00-07-2009

End Date: 00-11-2010

Program Area: Variety and Species

Commodity: Rice

Objectives:

To identify salt tolerant rice genotypes using molecular markers and to introgress saltol gene into Binadhan-7 with marker assisted selection technique.

Methodology:

Salt tolerant genotype FL-378 was selected as donor parent and Binadhan-7 was selected as recurrent or recipient parent. The experiment was conducted on the plastic pot (10L bucket) and pans designed by IRRI. The plants were labeled with pot labeler. For the identification of salt tolerant rice genotypes with marker assisted backcrossing (MABC) technique, the experiment was conducted during the period from July 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. Binadhan-7 was an early and high yielding salt susceptible mutant variety which was crossed with FL 378 which was an advanced salt tolerant line from IRRI. Binadhan-7 was the recurrent parent and FL 378 was the non-recurrent donor line. Seeds of selected F₁ lines were grown in the pot. Then F₁ plants were crossed with Binadhan-7 as a principle of backcrossing and 150 BC₁ F₁ seeds were developed. Among these seeds, 105 seeds were germinated in the experimental field in the next season. Among these 105 seedlings 72 seedlings were selected to collect the leaf samples to apply foreground selection using SSR markers. Hybridization was carried out between Binadhan-7 and FL-378 . Binadhan-7 was used as female parent while FL-378 as male parent in the hybridization scheme. Three sets parental lines were seeded at an interval of 14 days starting from July 5, 2009. Twenty- one days old seedlings were transplanted in the hybridization block. The size (in nucleotide base pairs) of the most intensely amplified band for each microsatellite marker was determined based on its migration relative to a molecular weight size marker (1kb+ DNA ladder) with the help of Alpha Ease 4.0 software. The band having same level of Binadhan-7 was scored as ‘A’ which indicated the homozygous allele of the recipient parent for the particular SSR marker. Again, the band having same level of FL-378 was scored as ‘B’ which indicated the homozygous allele of the donor parent for the particular SSR marker. However, heterozygous alleles were scored as ‘H’ having both the bands of two parents. Score ‘N’ indicated the absence of band. Importantly, heterozygous alleles always had the extra bands in most cases with bigger size.

Source of Information: The Agriculturists 10(2): 1-8 (2012),ISSN-1729-5211

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Based on the foreground selection with a tightly linked salt tolerance marker RM21, we selected 33 plants showing ‘H’ scores were selected. The selected segregants were subjected to further recombinant and background selections in BC₁F₁ generation. And further foreground, recombinant and background selections will be done with appropriate markers up to BC₃ or more for the development of salt tolerant rice lines and will be reported elsewhere.

Knowledge Management: Journal