Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Micropropagation of Vanda Orchid

M. Mahmuda Khatun
Scientific Officer
Tissue Culture Section, HRC, BARI, Gazipur

D. Khanam
Scientific Officer
Tissue Culture Section, HRC, BARI, Gazipur

M. A. Hoque
Principle Scientific Officer
Tissue Culture Section, HRC, BARI, Gazipur

Abstract/ Executive Summary:

The mature pods of Vanda sp. were used as explant. MS medium with different supplements and additives such as MS + 0.2 g/l charcoal, MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal and MS + without charcoal were tested for large- scale multiplication of native orchid, Vanda sp. Among these, MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal was found to be the best for seed germination, protocorm formation and plantlet development. In this medium, minimum days were required for seed germination (45 – 50 days) and plantlet formation (64-68 days). The highest shoot length (4.3 cm), leaf number(4.2), leaf length(3.1 cm), leaf width(0.4 cm), root length(1.8 cm) were also obtained in MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal. Root number was found higher when media were supplemented with charcoal. The activated charcoal enhanced root formation. In vitro raised plantlets were established in plastic pots with coconut fibre.

Keywords: Orchid, Vanda, Micropropagation, Charcoal, BAP, IAA

Location: Tissue Culture Section, HRC, BARI, Gazipur

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Orchid

Objectives:

To find out the suitable media for large scale multiplication and faster development of vanda orchid

Methodology:

The mature pods of Vanda sp. were collected and surface sterilized by submerging them in 0.1% HgCl₂ solution with 2-3 drops of Tween-20 for 15 min. The pods were washed 3-4 times with sterile distilled water and were transferred to a sterile plastic pot in laminar air flow cabinet. The pods were then dipped in absolute ethanol and flamed rapidly for a few seconds holding with forceps. They were then splitted longitudinally with a sterile scalpel and the seeds were taken by forceps and transferred to the agar solidified nutrient medium for seed germination.Three different culture media namely, MS (Murashige and Skoog, 1962) + 0.2 g/l charcoal, MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal and MS + without charcoal were used. In per litre of the medium 30 g sucrose and 8.0 g of agar were used and pH was adjusted to 5.8 prior to autoclaving. Seeds were sown in culture flask (50 ml) containing 20 ml of the medium. Culture flasks (100 ml) containing 35 ml of fresh medium were used for subculturing. The culture vessels were autoclaved at 121⁰C for 25 minutes at 1.2 kg/cm² pressure. The inoculated culture vessels were incubated at 25⁰C ± 1⁰C under 16h photoperiod with a light intensity of approximately about 2000 lux under white fluorescent tubes.

Source of Information: Annual Report of Biotechnology Division (BARI)Annual Report, 1996-1997, Gazipur

Article Link (Online Journal):

Funding Source:

1.	Budget: 15,000/- (Fifteen thousand taka only)

Total Budget (Tk.) : 15,000/- (Fifteen thousand taka only)

Achievement/Findings:

Seed germination was recorded in all the treatments within 55 days. It was observed that maximum days (53 – 55) were needed for seed germination in MS medium without charcoal supplementation and minimum days (45 - 50) were required in MS media containing 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal. Results showed that protocorm formation started 60 – 68 days both in charcoal supplemented media (MS + 0.2 g/l charcoal and MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal).Shoot initiation was observed within 50-55 days in MS + 0.2 g/l charcoal and 54-58 days in MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal while in 60 – 62 days needed in without charcoal supplemented media. The minimum days (64 – 68) were required for plantlet formation in MS + 2.0 g/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal whereas 68 - 70 days in MS + 0.2 g/l charcoal.In order to induce rapid growth and development, protocorm were transferred to different culture media with or without charcoal.The highest shoot length (4.3 cm) was observed in MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal followed by MS + without charcoal (2.5 cm). The highest leaf number (4.2) was obtained from the treatment containing MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal followed by MS + 0.2 g/l charcoal (3.1). The highest leaf length (3.1 cm) was observed from MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal followed by MS + 0.2 g/l charcoal (1.5 cm).The highest width of leaf (0.4 cm) was observed from MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal followed by MS + 0.2 g/l charcoal (0.3 cm). The highest number of roots (3.6) was observed in MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal and lowest from MS + 0.2 g/l charcoal (2.2).On the other hand, MS + without charcoal did not produce any root. Results indicated that medium supplemented with activated charcoal found best for number of root formation. The highest root length (1.8 cm) was found in MS + 2.0 mg/l BAP + 1.0 mg/l IAA + 0.2 g/l charcoal.

Knowledge Management: Report/Proceedings