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Milt Quality Determination of a Critically Endangered Fish, Olive Barb (puntius Sarana, Ham.) in Bangladesh

M.U.H. Khanam
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh 2202

M. Nahiduzzaman
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh 2202

M.M. Hassan
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh 2202

M. Sultana
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh 2202

S.M. Rafiquzzaman
Freshwater Station, Bangladesh Fisheries Research Institute, Mymensingh 2201

M.A.R. Hossain
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh 2202

Abstract/ Executive Summary:

The present study was aimed to evaluate the characteristics of the olive barb sperm. Milt was collected fortnightly from 49 male fish (mean weight 90.8 g and length 18.64 cm) from April to July in 2008. In the olive barb ejaculated milt, volume (μl/g), motility (%), duration of motility (s), concentration (x 10¹⁰/ml) and pH values were found to be 6.06±0.32, 88.27±0.71, 171.41 ± 7.41, 5.16±0.05 and 7.75±0.04, respectively. Milt volume was significantly (P<0.05) correlated with sperm concentration. Milt volume, sperm concentration, motility and duration of motility significantly varied (P < 0.05) during spawning season.

Keywords: Puntius sarana, Olive barb, Endangered, Milt quality

Location: Chalan beel, Natore district and Tola haor, Netrokona district.

Start Date: 00-01-2008

End Date: 00-02-2008

Program Area: Quality and Nutrition

Commodity: Fish

Objectives:

To evaluate the sperm quality of olive barb Puntius sarana.

Methodology:

In the present study, Puntius sarana was collected from two natural depressions (Chalan beel, Natore district and Tola haor, Netrokona) during the month of January and February, 2008. The average size of male fish was 70g and 110g in Chalan beel and the Tola haor, respectively. The collected fish were reared in the ponds of Fisheries Field Complex, Bangladesh Agricultural University, Mymensingh. Fish were collected from the brood rearing ponds and kept in the tank of the hatchery of Faculty of Fisheries. Then collected broods were injected with PG at the rate of 2 mg PG/kg body weight for easy collection of milt samples. For sperm collection at first males were fished out from the tank using scoop net. Then fish was laid on the foam fixing in dorsal position and urogenital pore was wiped. Gentle pressure was applieed through the abdomen to remove urine, water, gut exudates and mucus and these were removed with tissue as much as possible for avoiding contamination. A 3 ml plastic syringe was used to collect the sperm. When the milt seemed concentrated, the mouth of the syringe was inserted into the urogenital pore to fill with sperm and then sperm was immediately transferred to the icebox. After collection, sperm samples were transported to the laboratory under cold conditions (7-10 °C). Ejaculated sperm volume was determined by the measuring pipette and expressed as μl. Milt pH were determined with a pH indicator strips (pH: 0-14; Merck, Germany). Sperm motility was evaluated visually for the percent motility (%) after activation in table salt (NaCl). The duration of motility (sec) was also recorded by stopwatch from the initial contact between the activation solution and milt until almost all of the spermatozoa (up to 20%) were immotile. One or two drop 0.9% NaCl was placed on a glass slide and then a drop of 1-2 μl fresh milt was poured to induce the initiation of motility. A light microscope (Novex K-range, Holland) was used at 400 magnifications to determine the percent motility. Sperm concentration was determined using haemacytometer (Germany) and expressed as number of cells x 10¹⁰/ml. Milt was diluted 4,000 times in a 0.9% NaCl solution. For preparing 4,000 times dilution, at first microtube containing 990 μl of the 0.9 % NaCl solution was taken and then 10 μl of milt was carefully added to the tube and the content was mixed carefully. From this microtube, 10 μl of milt suspension was taken out and transferred to another microtube containing 390 μl of 0.9 % NaCl solution and finally 4,000 times diluted milt was prepared. A droplet of the diluted milt was placed on a haemocytometer (depth 0.1 mm) with cover slip. The slide was left undisturbed for approximately S min to allow the milt cells to settle on focal plane. The number of milt in S large squares of the counting chamber was counted under the microscope at 40 times magnification.

Source of Information: Bangladesh J. Fish. Res., 12 (2), 2008: 163-171

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the olive barb ejaculated milt, volume (μl/g), motility (%), duration of motility (s), concentration (x 10¹⁰/ml) and pH values were found to be 6.06±0.32, 88.27±0.71, 171.41 ± 7.41, 5.16±0.05 and 7.75±0.04, respectively. Milt volume, sperm concentration, motility and duration of motility significantly varied during spawning season.

Knowledge Management: Journal