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Tissue Specific Esterase Isozyme Variation in Clarias Batrachus and C. Gariepinus

Md. Abdur Rashid
Genetics and Molecular Biology Laboratory, Department of Zoology University of Dhaka, Dhaka-1000, Bangladesh.

Dilshad Tamanna Rahman
Genetics and Molecular Biology Laboratory, Department of Zoology University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

Esterase isozymes of Clarias batrachus and C. gariepinus were studied on 7.5% Polyacrilamide Gel Electrophoresis (PAGE) stained with alfa and bita naphthyl acetates as substrates to see the tissue specific variation of this enzyme from nineteen different tissues. Maximum six esterase bands (Est-1^1.52±0.01, Est-2^1.26±0.01, Est-3^0.99±0.01, Est-4^0.67±0.02, Est-5^0.33±0.01 and Est-6^0.17±0.02) were observed in Asian catfish (C. batrachus) where as African catfish (C. gariepinus) shown only four (Est-1^1.43±0.02, Est-2^1.22±0.03, Est-3^1.02±0.02 and Est-5^0.33±0.02). Localization of esterase isozymes were observed in different tissues of both Asian and African catfishes that indicated the switch on and off of specific allele based on the physio-chemical condition of corresponding tissues. Relatively higher concentration of esterase isozymes were found in digestive tissues. High number of esterase bands was found in Asian catfish which seems to indicate the higher allelic variation than in African catfish.

Keywords: Electrophoresis, Esterase isozymes, Tissue specificity, Clarias batrachus, C. gariepinus

Location: laboratory of Genetics and Molecular Biology, Department of Zoology, University of Dhaka.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Cat fish

Objectives:

To find out tissue specific esterase isozyme banding pattern in different tissues of Asian (C. batrachus) and African catfish (C. gariepinus).

Methodology:

Samples were collected from New Market, Dhaka, Bangladesh (original source-unknown) and the experiments were conducted in the laboratory of Genetics and Molecular Biology, Department of Zoology, University of Dhaka. Nineteen different tissues namely Anterior, Mid, Tail (in ventral region), Tail (in tip region), Buccal and Pelvic muscle, Fore, Mid and Hind gut and brain, Liver, Stomach, Kidney, Gill, Heart, Eye (lens, black portion) were taken (~ 0.016 g) from both species to observe the tissue specific esterase isozyme variability. Each tissue was squashed in TBE buffer (40 μl) and aliquots from each sample (15 μl) were loaded on the separate gel slots for electrophoresis after centrifuged at 12500 rpm for 15 min. The entire technique for polyacrylamide gel electrophoresis (PAGE) and the electrophoretic bands of esterase isozymes resulting from stained gel with alfa and bita naphthyl acetates were assigned to increasing numbers based on decreasing mobility. The experiment was repeated to standardize the result with different specimens. As there were no significant variations in each repetition, only one repetition was subjected to analysis.

Source of Information: Journal of Entomology and Zoology Studies Vol. 1 No. 2, 1-7, 2013

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Esterase studies are essential to know the genetic make up of a species for any successful conservation effort. Present study may be used for the development and application of molecular markers as for example species identification, selection and toxicological status.

Knowledge Management: Journal