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Tissue Specific Esterase Isozyme Variation in Four Punctius Species

Mohammad Mustafa
Genetics and Molecular Biology Lab. Department of Zoology, University of Dhaka, Bangladesh

MD Abdur Rashid
Genetics and Molecular Biology Lab. Department of Zoology, University of Dhaka, Bangladesh

Zannatul Ferdous
Genetics and Molecular Biology Lab. Department of Zoology, University of Dhaka, Bangladesh

Mohammad Shamimul Alam
Genetics and Molecular Biology Lab. Department of Zoology, University of Dhaka, Bangladesh

Rowshan Ara Begum
Genetics and Molecular Biology Lab. Department of Zoology, University of Dhaka, Bangladesh

Reza MD Shahjahan
Genetics and Molecular Biology Lab. Department of Zoology, University of Dhaka, Bangladesh

Abstract/ Executive Summary:

Electrphoretic banding pattern of esterase isozymes was studied on 7.5% polyacrylamide gels from ten different tissues of four Punctius species (P. sarana, P. sophore, P. conchonius and P. ticto). All the studied species showed five esterase bands except P. ticto that lacked Est-5. Maximum number of bands was observed in heart (80%), liver (75%), pelvic muscle (73.75%), eye (70%) and brain (68.75%) of four mentioned species whereas, minimum in anterior muscle (47.5%). Same tissue of different species showed both similarity and dissimilarity in terms of allelic expression. As for example, four esterase bands were found in the liver of P. conchonius (Est-1, Est-3, Est-4 and Est-5) and P. ticto (Est-1, Est-2, Est-3 and Est-4) while two and three bands in P. sophore (Est-3 and Est-4) and P. sarana (Est-2, Est-3 and Est-4) respectively. On the other hand, allele of certain locus also showed tissue and species specific expression with varied type of intensity. For example, Est-4 was common in the liver of all the species and its intensity varied from faint to deep whereas, deep stained Est-5 was confined to the same tissue of P. conchonius only.

Keywords: Electrophoresis, Esterase Isozymes, Tissue Specificity, Punctius spp.

Location: Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Aquatic animal

Objectives:

To investigate the tissue specific esterase isozyme variation in the studied species.

Methodology:

The present study on esterase loci from different tissues of Punctius species was carried out in Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka, Bangladesh. The adult fish samples were collected from Titas River, Brahmanbaria and were transported to the laboratory with ice cool packs. The specimens were then dissected to collect the desired amount (~0.016 g) of following tissues: Pelvic_, Anterior_ and Tail_ muscle, Eye, Brain, Liver, Stomach, Heart, Fore_ and Mid_ gut of intestine. Each tissue was squashed in TBE buffer (40 μl) and aliquots from each sample (15 μl) were loaded on the separate gel slots for electrophoresis after centrifuged at 12500 rpm for 15 min. The electrophoresis was done on the continuous supply of 120 V and 300 mA. The entire technique for polyacrylamide gel electrophoresis (PAGE) and the electrophoretic bands of esterase isozymes resulting from stained gel with alfa and bita naphthyl acetates were assigned to increasing numbers based on decreasing mobility. The experiment was repeated to standardize the result with different specimens. As there was no significant variation in each repetition, only one repetition was subjected to analysis.

Source of Information: JOURNAL OF CURRENT RESEARCH IN SCIENCE Vol. 1, No. 4, pp:254-259, 2013

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Expression of tissue specific esterase isozymes showed differential banding pattern that could be used in rapid and inexpensive identification of fish species, in toxicological study and to develop molecular markers but it needs further intensive study.

Knowledge Management: Journal