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Tissue Specific Esterase Isozyme Variation in Ocellated Pufferfish, Tetraodon Cutcutia

Sabekun Nahar
Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka, Bangladesh

Md. Abdur Rashid
Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka, Bangladesh

Abstract/ Executive Summary:

Variation in the expression of esterase isozymes was studied from twenty different tissues of Tetraodon cutcutia on 7.5% polyacrylamide gel electrophoresis (PAGE) stained with alfa and bita naphthyl acetates. A variant type of allelic expressions was observed in different tissues where altogether, five esterase bands (Est-1, Est-2, Est-3, Est-4 and Est-5) were detected. Est-4 showed the highest frequency (95%) whereas Est-3 was found to be lowest (30%). Localization of esterase isozymes were also observed in different tissues that showed tissue specific isozyme variation. Tissues that were related to digestive system (liver, intestine) showed higher allelic variation (80%). All the bands were present only in eye tissue. One the other hand, only one band (Est-4) was present in anterior_ and mid_muscle. Est-3 was absent in digestive, nerve and muscle tissues but was present in skin, fin, eye and adipose tissues.

Keywords: PAGE, Esterase isozyme, Tissue specificity, Tetraodon cutcutia

Location: Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka.

Start Date: 00-00-2012

End Date: 00-00-2012

Program Area: Variety and Species

Commodity: Aquatic animal

Objectives:

To investigate the tissue specific esterase isozyme variation in the studied species.

Methodology:

The present study on esterase loci from different tissues of Tetraodon cutcutia was carried out in Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka, Bangladesh. The adult fish samples were caught from the ponds of Gopalgonj and were transported to the laboratory with ice cool packs. The specimen was then dissected to collect the following tissues: Liver, Stomach, Intestine, Brain, Eye, Kidney, Ovary, Heart, Gill, Buccal_, Lip_, Anterior_, Mid_ and Posterior_muscle, Lip_, Anterior_, Mid_and posterior_skin, Adipose tissue and Tail fin. Each tissue was squashed in TBE buffer (40 micro l) and aliquots from each sample (15 micro l) were loaded on the separate gel slots for electrophoresis after centrifuged at 12500 rpm for 15 min. The electrophoresis was done on the continuous supply of 120 V and 300 mA. The entire technique for polyacrylamide gel electrophoresis (PAGE) and the electrophoretic bands of esterase isozymes resulting from stained gel with alfa and bita naphthyl acetates were assigned to increasing numbers based on decreasing mobility. The experiment was repeated to standardize the result with different specimens. As there was no significant variation in each repetition, only one repetition was subjected to analysis.

Source of Information: Journal of Entomology and Zoology Studies 2013; 1 (5): 38-42

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Expression of tissue specific esterase isozymes showed differential banding pattern that could be used in toxicological study and also could be used for the development of molecular markers.

Knowledge Management: Journal