Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Dose Optimization with Synthetic Hormone Flash for Induced Spawning of Shing (heteropneustes Fossilis)

Md. Munsur Ali
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abdulla-Al-Asif
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md. Azharul Islam Sabuj
Department of Fisheries and Marine Bioscience, Faculty of Biological Science and Technology, Jessore University Of Science and Technology, Jessore-7408, Bangladesh.

Omar Faruq
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Suvashis Vaumik
Farm Officer, Sherpur Fish Hatchery, CP Bangladesh Co. Ltd. Balughata, Nalitabari, Sherpur.

Md. Abu Zafar
Department of Aquaculture, Hajee Mohammad Danesh Science and Technology University, Dinajpur. Bangladesh.

B.M. Newaz Sharif
Department of Aquaculture, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

An experiment on induced breeding of Heteropneustes fossilis was carried out to determine the optimum dose of Flash hormone at the Bogura Matshya Khamar, Parulia, Palash, Norshindhi. The present study consisted of three treatments (T1, T2 and T3) with nine replications of each. Forty five pairs of male and female were selected from the brood rearing ponds and the average body weight of the female and male were 100±10 g and 55±5 g respectively. To observe the effective dose for induced breeding, the females were injected at the rate of 0.5 (T1), 0.45 (T2) and 0.42 (T3) ml Flash/kg body weight and correspondingly the males were administered a dose of 0.20 (T1), 0.18 (T2) and 0.17 (T3) ml Flash/kg body weight in all treatments. The fertilization rate and hatching rate were determined. Then the hatchlings were reared in aquarium up to 7 days and survival rate was determined. The water temperature was recorded between 26 to 31⁰C during experimentation. Among the three treatments T1 showed the best result in terms offertilization rate (80.33%), hatching rate (71.67%) and survival rate (61.56%). The present findings can be used in induced breeding of Heteropneustes fossilis for the development of hatchery propagation.

Keywords: Dose optimization, Synthetic hormone flash,Induce spawning, Shing

Location: Narshindhi, Bangladesh.

Start Date: 01-08-2015

End Date: 10-09-2015

Program Area: Variety and Species

Commodity: Cat fish

Objectives:

To determine the optimum dose of the species to determine the optimum dose of “Flash (containing sGnRH + dopamin)” for induced breeding of Shing; and the fertilization, hatching and survivability rate of Shing.

Methodology:

Experimental site: The research work was conducted at the Bogura Matshya Khamar Complex, Parulia, Palash, Narshindhi. The experiment was performed during 1st August to 10th September 2015. Facilities present in Bogura Matshya Khamar Complex: During the breeding period different infrastructure of the hatchery was used to complete the experiment such as rectangular tank, overhead tank etc. Collection of fish: Two hundred and fifty five Shing were collected from the own brood pond. The collected fish were stocked in the rearing pond situated inside the hatchery complex, during 1st August to 10th September 2015. Brood pond preparation: Brood fish is called as the heart of the hatchery and management practices of brood stock is the important step for good seed production. Artificial breeding mainly depends on sufficient amount of quality brood fishes. Brood stocks were managed scientifically so that mature broods could be obtained during the full breeding season. There were 4 brood ponds each having an average area of 30 decimal and the water depth between 1.0-1.5 meters. For preparation of the brood ponds, the usual practice was to eradicate the predator and the unwanted fishes by dewatering and drying. Rotenone and phostoxin were also used to remove the unwanted fish species and aquatic insects. Aquatic vegetation was removed manually. After cleaning the pond, lime was used at the rate of 1 Kg/decimal and 5-7 days after liming, cow dung was applied at the rate of 5-7 Kg/decimal as organic fertilizer. At the same time inorganic fertilizers such as Urea and TSP were also used at the rate of 150 g and 100 g per decimal respectively. Seven days after fertilization, fish were released in brood pond with intensive care. Feeding was done with formulated feed. In this formulated feed 20 -25% of protein level was maintained. The hatchery owners used aluminum pot to carry the brood fish from brood pond to hatchery unit. Selection of brood fish: Brood fishes were selected for brood stocking considering size, shape and color. Only the ready to spawn breeders were selected for breeding trials. The broods were easily identified on the basis of external feature of their abdomen and pectoral fins. Their enlarge abdomen with a softy touch (during the breeding season) enable them to be identified correctly. The average weight of the females and males were 100±10 gram and 55±5 gram respectively. Conditioning: Mature males and females from the brood rearing ponds were selected and immediately carried to the hatchery. Male and female fishes were kept into separate hapa in rectangular tank for about 5 hours. They were subjected to induce the breeding condition under water showering. Water flow was maintained to ensure sufficient aeration. No feed was provided during the period of conditioning. Experimental design: Three treatments were considered for the experiment in each treatment nine replications were considered and total 90 fishes were used. Collection and preparation of “Flash”: Locally available Flash was collected from market in preserved condition in airtight vials. At first, the required amount of Flash was taken in a small bowl by syringe which has a capacity of 1 ml (100 Unit). Then sufficient amount of water added to the Flash to dilute the solution. Hormone administration: After preparation of Flash solution, brood fishes were caught carefully by net, and kept in sponge. Then the inducing agent was injected near the pectoral fin base. The amount of Flash solution for each fish was determined before injection according to the body weight of the broods. After injection male and female were kept in hapa where they released eggs automatically after 8-12 hours depending on the treated doses. For dose optimization fertilization rate, hatching rate and survival rate were determined. Doses of Flash for male and female brood: Dose of Flash was administered to the female and male broods in between 5:00 to 6:00 pm. Then the fishes were kept into hapa with proper aeration until they release their eggs. Collection of fertilized eggs and transferring to hatching tank: After spawning the brood fishes were removed from the hapa by net and transferred into the ponds. The eggs were kept there to hatch. Determination of fertilization rate: To determine the fertilization rate 100 eggs was taken in a Petridis from hatching jar. Then the eggs were observed under a magnifying glass and fertilized eggs were counted. The fertilized eggs are not transparent as the hatching egg. Their color is slightly brownish. The fertilized eggs were easily separated from the unfertilized eggs by the presence of transparent shell with gray spot within the eggshell, while the unfertilized eggs were opaque. The fertilization was determined. Determination of hatching rate: Hatching was completed after 22±2 hours of. To determine hatching rate 100 fertilized eggs were collected in a tray and the total numbers of the hatchlings were counted by visual observations. The hatching rate was determined. First feeding: The yolk sac absorption was completed after 70±2 h of hatching. After 72 h of post-hatching boiled egg-yolk provided as first food for the hatchling of Shing at ambient temperature of 22 to 26 0C. At that time they were little bit of blackish and transparent in appearance. Determination of survival rate: Newly hatched fry are observed for seven days to see survivability. All other conditions during the experimentation were maintained same. After completion of the experiment at 7th day, the number of total live larvae in the tray was counted separately for calculation of survival rate. Statistical analysis: The results found in the experiment were subjected to statistical analysis, ANOVA, (one way) that showed the significance (P<0.05) level of differences between the treatments. This statistical analysis was performed with the aid of the computer software SPSS version 16. Significant results (p<0.05) were further tested using Duncan’s New Multiple Range Test (DMRT) to identify significant differences among means.

Source of Information: International Journal of Fauna and Biological Studies 2015; 3(1): 39-45 ISSN 2347-2677

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Among the three treatments T1 showed the best result in terms of fertilization rate (80.33%), hatching rate (71.67%) and survival rate (61.56%). The present findings can be used in induced breeding of Heteropneustes fossilis for the development of hatchery propagation.

Knowledge Management: Journal