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Bacteriological Quality of Tanualosa Ilisha (hamilton) in a Freezing Plant

Shaista Saeed
MS Student

Md. Motaher Hossain
Research Officer
Matshya Bhaban, Ramna, Dhaka

Abstract/ Executive Summary:

Bacteriological analyses were carried out to determine the quality of Tanualosa ilishe in a freezing plant.The viable cell counts of different groups of bacteria in the flesh of frozen hilsha fish, unfrozen hilsha fish and related environments were determined by standard methods. The total viable bacterial count, Coliform and faecal coliform in the flesh of unfrozen hilsha fish were found higher than that found in the fresh frozen hilsha fish. From the experimental findings it could be seen that the standard plate count(SPC), Coliform and faecal coliform in case of unfrozen hilsha fish of the two markets namely New market and Kawran bazar did not comply with the International Commission on the Microbiological Specification of Food (ICMSF) standard. But the same results of the other two fish markets complied with ICMSF standard.Vibrio cholerae and Salmonella spp. were absent totally in all the samples. SPC of the frozen fish was lower than that of unfrozen hilsha fish collected from different markets and fish habitats.The SPC of water varied in the range of 226.5/ml to 1630 /ml. In case of flake ice, the SPC varied between 595 /ml and 1360/ml. Coliform was found in flake ice 25/ml. The SPC of Swab was 195/cm square, and the same of the hand of the panning worker was 225/ square cm. But one lot of frozen hilsha fish contained 15 faecal coliforms per gm. This finding suggests that it is very much essential to check frozen fish for faecal coliform at regular intervals. Vibrio cholerae and Salmonella spp. were not detected in any of the samples studied.

Keywords: Hilsha fish, Bacteriological quality,Microbiological test, Tanualosa ilisha, Freezing plant.

Location: Fish Inspection and Quality Control Laborator, Dept. of Fisheries

Start Date: 01-07-2000

End Date: 30-06-2001

Program Area: Food Safety and Security

Commodity: Hilsa

Objectives:

1. To determine the total bacterial load of frozen hilsha of a Snowking freezing plant and unfrozen hilsha of different fish markets.

2. To determine the total and faecal coliform

3. To detect salmonella and Vibrio cholerae for the assurance of quality of frozen and unfrozen hilsha for export.

Methodology:

Twelve frozen hilsha samples were collected lot wise from Snowking freezing plant , Mirpur, Dhaka and twelve unfrozen hilsha fishes were collected from Chandpur and local markets of Dhaka.Water samples were collected from taps and panning table of freezing plant and flake ice samples were collected from ice store of the freezing plant during the collection of fish samples and carried to the Laboratory of Fish Inspection and Quality Control Laboratory, DoF, Dhaka for analyses. Moreover swab samples were collected from the surface of the panning tray and the surface of workers hands of the freezing plant.Total plate count was determined by ten fold serial dilution method and calculated as cfu/ml. Coliform and faecal coliform counts were determined by Most Probable number Method. Detection of Salmonella and Vibrio cholera were done by using respective agars.

Source of Information: Dhaka Univ. J. Biol. Sci. 12(2): 223-227, 2003 (July)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It was observed that, in all samples SPC was higher than that recommended by EU standard. Thus it is crucial that, the issue of proper checking at the processing plant is vital especially for export purpose.

Knowledge Management: Journal