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Antimicrobial Activity of Leaf Extracts of Eupatorium Triplinerve Vehl. Against Some Human Pathogenic Bacteria and Phytopathogenic Fungi

MD. SHAFIQUR RAHMAN
Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh

MOHAMMAD JUNAID
Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh

Abstract/ Executive Summary:

Petroleum ether, carbon tetrachloride, chloroform, and ethyl acetate extracts of Eupatorium triplinerve Vehl. leaves were investigated for their antimicrobial activities against 11 human pathogenic bacteria and six phytopathogenic fungi. The crude extracts showed good activity against the organisms tested herein. The chloroform extract exhibited the largest zone of inhibition (22 mm in diam with 1000 μg/disc extract) against Vibrio and the highest inhibition of fungal radial mycelial growth (73.5% with 100 μg extract/ml medium) against Colletotrichum corchori. The chloroform extract exhibited the lowest MIC against Vibrio (250 μg/ml) and C. corchori (62.5 μg/ml). It appeared that E. triplinerve could be a potential natural source of new antimicrobial agent.

Keywords: Antimicrobial activity, Leaf extract, Eupatorium triplinerve

Location: Chittagong University campus, Chittagong, Bangladesh

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Tree

Objectives:

To evaluate the antimicrobial activity of leaf extracts of Eupatorium triplinerve V.

Methodology:

Fresh leaves of Eupatorium triplinerve were collected from Chittagong University campus, Chittagong, Bangladesh. The cleaned leaf samples were cut into small pieces (1-2 cm), air dried and ground to fine powder mechanically. 50 g of the dried powders were kept overnight in petroleum ether, chloroform, ethyl acetate and carbon tetrachloride. The extracts thus obtained were filtered, centrifuged at 5000 rpm for 20 minutes and then concentrated to a gummy material under reduced pressure at 50º C by rotary vacuum evaporator. The gummy material was then transferred to small vials and dried and termed as crude extract.

Source of Information: Bangladesh J. Bot. 37(1): 89-92, 2008 (June)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It appeared that the crude extracts inhibited the radial mycelial growth of all the test fungi at a concentration of 100 μg/ml medium to varying degrees. The crude extracts exhibited prominent (more than 50%) inhibitions of radial mycelial growth against all the test fungi except Botryodiplodia theobromae with carbon tetrachloride, chloroform and ethyl acetate extract, respectively. The highest inhibition (73.5%) of fungal radial mycelial growth was recorded against Colletotrichum corchori at a concentration of 100 μg/ml medium using the chloroform.

Knowledge Management: Journal