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Temporal Variation in Seed Quality of Indian Spinach Preserved in Different Containers

M. Kamruzzaman
Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh-2202, Bangladesh

S. Khatun*
Scientific Officer
Bangladesh Institute of Nuclear Agriculture BINA Sub- station, Ishurdi, Pabna, Bangladesh

A. Rakib
Scientific Officer
Bangladesh Institute of Nuclear Agriculture BINA Sub- station, Ishurdi, Pabna, Bangladesh

M.I. Hoque
Scientific Officer
Bangladesh Institute of Nuclear Agriculture BINA Sub- station, Ishurdi, Pabna, Bangladesh

M.H. Rani
Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted to investigate the seed quality of Indian spinach for three months stored at different containers (Plastic pot, polythene bag and cloth bag) during 2013-2014 at Seed Technology Laboratory of Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur. The initial moisture content of the seed was 9.89% with 75.00% germination. However, after two months, the seed had a different range of increased moisture content with reduced germination percentage depending upon the packing materials. Among the three containers, seeds stored at cloth bag absorbed more moisture (14.36%) from surrounding atmosphere followed by the moisture content, 12.67% and 11.50% of polythene bag and airtight plastic pot, respectively. Seeds stored at plastic pot, polythene bag and cloth bag exhibited 65.00, 64.00 and 50.00 per cent germination, respectively. Similarly, the fresh weight and dry weight of seedling and seedling vigor were also decreased. The seeds of plastic pot were good in term of germination capacity, fresh and dry weight and vigor index in comparison to those stored at cloth bag and polythene bag.

Keywords: Germination Percentage, Moisture Content, Fresh and Dry weight

Location: Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh

Start Date: 00-00-2013

End Date: 00-00-2014

Program Area: Seed Technology

Commodity: Indian spinach

Objectives:

To evaluate the germination percentage of seeds, viability and vigor of seedlings of Indian spinach contained in different containers;
To know the effect of moisture on the germination percentage of Indian spinach seed.

Methodology:

After recording the initial moisture content and germination percentage, seeds were kept in three containers: plastic pot, polythene bag and cloth bag and stored at room temperature (25°C) and RH for two months. Germination test and recording the moisture content (wet basis) was performed every 20 days interval beginning from collection of seeds up to 60 days to observe the rate of natural ageing in different containers. Moisture content was determined by using high constant temperature oven method following International Rules for Seed Testing (ISTA, 2015) in the Agronomy Laboratory of BSMRAU. Seeds of 5.0 g were taken from each three containers, poured in a small container with cover, and kept in an oven maintained at a temperature of 130^oC for a period of 1 hour. The moisture content of seeds (wet basis) was determined. Germination test was conducted by following sand method. For germination, test seeds were soaked in water for overnight and placed in plastic tray with moist sand. 400 seeds were taken randomly from the seed samples of each container. 100 seeds were placed on a plastic tray with 4 replications. The plastic trays with seed were incubated at room temperature. After 4 days of incubation first count were done and after 14 days final count was recorded following ISTA rules. Germination percentage was calculated. Seedlings obtained from standard germination test were used for seedling evaluation. Normal or abnormal seedlings were classified according to the rules of ISTA (2015). Seedling shoot and root length was measured on day 14 of the germination test. Ten plant samples from each plastic pot were harvested and shoot and root length of individual plant was recorded and their wet weight and dry weight were measured. After taking fresh weight, samples were kept in a drying oven for 72 hours at 70ºC temperature. For observation of biotic factor that influence seed quality, 20 g of Indian spinach seeds were taken and observed the numbers and kind of insects or pathogen were present. No biotic factors like insects and pathogen was observed during the storage period in all the containers. It might be due to the storage period was short (around 2 months) and the storage climate was not favorable for insects and pathogen activity for Indian spinach seeds. Recording of weather data Reading of dry bulb and weight bulb temperature and relative humidity of inside the room and outside the room was recorded in 9.00 AM and 5.00 PM a day. Maximum and minimum temperatures were recorded from outreach room conditions of BSMRAU.

Source of Information: Int. J. Agril. Res. Innov. & Tech. 5 (2): 51-57, December, 2015 ISSN: 2224-0616

Article Link (Online Journal): http://www.banglajol.info/index.php/IJARIT/article/view/26271/17649

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study revealed that the best container for the Indian spinach’s seed preservation was plastic pot. The seed stored in plastic pot absorbed low moisture from atmosphere and showed low seed dead and abnormal seedling rate. On the other hand, seed stored in plastic pot gave the maximum germination percentage, high seedling vigor and best seedling growth. So, the plastic pot might be recommended for the preservation of Indian spinach’s seed.

Knowledge Management: Journal