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Growth Performance and Nutritional Analysis of Spirulina Platensis in Different Concentrations of Papaya Skin Powder Media

M. A. Toyub
Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh

M. Z. Uddin
Department of Fisheries, Ministry of Fisheries and Livestock, Bangladesh

M. I. Miah
Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, Bangladesh

M. A. B. Habib
Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, Bangladesh

Abstract/ Executive Summary:

The growth performance of Spirulina platensis was studied in four different concentrations viz. 0.3, 0.4, 0.5 and 0.6 g/l of papaya (Carica papaya) skin powder media (PSPM) and in control, Kosaric Medium (KM) in triplicate. The initial cell weight of S. platensis was 6.0 mg/l which attained a maximum weight of 913.13 mg/l on the 10th day of culture in the control KM followed by 699.07, 612.13, 538.99 and 377.48 mg/l that grown in 0.40, 0.30, 0.50 and 0.60 g/l of PSPM respectively. Similar trend was observed in the case of chlorophyll a content ranging 3.57 to 8.15 mg/l. The specific growth rate (SGR, μ/day) of S. platensis on the basis of cell weight and chlorophyll a content was recorded in the ranges of 0.41 to 0.50 and 0.41 to 0.49, respectively, for all the treatments. The SGR on the basis of cell weight and chlorophyll a was recorded significantly (p<0.05) higher in KM among all the treatments. On the other hand, among the four different concentrations of PSPM, 0.40 g/l showed significantly (p<0.05) higher SGR than others. The pH and other physico-chemical factors were within suitable range of algae culture. The cultured microalga was nutritionally rich.

Keywords: Spirulina, Papaya skin, Cell weight, Chlorophyll

Location: Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh.

Start Date:

End Date:

Program Area: Food Safety and Security

Commodity: Fresh and processed food, Papaya

Objectives:

To study the growth performance of S. platensis in various concentrations of PSP and to analyze its nutritional value.

Methodology:

For collection of papaya skin, papaya was collected from local market and sun dried primarily and then in an oven at 40^oC for overnight. The dried skin was powdered and sieved with mesh size of 0.01 mm for getting fine particle. For preparation of different concentrations of papaya skin powder medium (PSPM) 0.3, 0.4, 0.5 and 0.6g of papaya skin powder was added per liter of distilled water and was then thoroughly mixed. Urea (0.2 g/l) was added to increase nitrogen content and NaHCO₃ (6.0 g/l) was added to increase pH of each concentration of the culture media since the initial pH were 7.6 - 7.8. Then the media were mixed well and sterilized at 120^oC for a period of 15 minutes with moist heat by autoclave and cooled for a period of 24 hours. Kosaric medium (KM) was used as control medium. Composition of KM modified form of Zarouk (1966) and Phang and Chu (1999). The prepared control medium was sterilized at 120^oC for a period of 15 minutes with moist heat by autoclave and cooled for a period of 24 hours. Stock culture of Spirulina platensis (Initially collected from Malaysia) maintained in the Department of Aquaculture, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh was used for this study. Dissolved oxygen (DO) and pH of the culture media were measured by oxygen meter (YSI, Model 58) and electric pH meter (Jenwey Model 3032), respectively. Temperature and light intensity of the culture media were recorded by using a Celsius thermometer and lux meter, respectively. The samples were filtered with filter paper (Whatman, GF/C) and shifted to the oven at 105^oC for 24 hours. Cell weight (mg/l) = Weight of the filter paper with sample - Weight of the filter paper without sample before filtering Optical densities of the prepared samples were determined at 664, 647 and 630 nm wave length by using UV spectrophotometer. A blank with 100% acetone was run simultaneously. Chlorophyll a content was calculated by the following formula: Chlorophyll a (mg/l) = 11.85 (OD 664) - 1.54 (OD 647) - 0.08 (OD 630). Mean and standard deviation were calculated from the experimental data. Then the data were analyzed through one way analysis of variance (ANOVA) using SPSS followed by Duncan's Multiple Range Test (DMRT).

Source of Information: Bangladesh J. Sci. Ind. Res. 46(3), 333-338, 2011

Article Link (Online Journal): www.banglajol.info

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It is concluded that the growth of S. platensis was maximum in 0.4 g/l PSPM than other media of the experiment. Thus, this concentration may be recommended for culture of S. platensis though the control KM gave the highest (P<0.05) growth.

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