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Karyotype of Some Species of Chara and Nitella (charophyta) from Bangladesh

WAHIDA KHATUN
Professor Sultanul Alam Cytogenetics Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

MD. MOSLEHUD-DEEN
Professor Sultanul Alam Cytogenetics Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

GOLAM KABIR
Professor Sultanul Alam Cytogenetics Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

Abstract/ Executive Summary:

Karyotypic analysis of Chara corallina Linn., C. vulgaris Linn., C. gymnopitys Linn. and Nitella translucens Ag. collected from natural habitats revealed that C. corallina has n=42, C. vulgaris and Chara gymnopitys have n=14 while Nitella translucens has n=18 chromosomes. All the species had differences in individual chromosome length, total chromatin length, total frequency between the complements of their chromosomes. The proposed standard karyotype formula were 6L^m + 2M^m +26M^sm + 6S₁^m + 2S₂^m for Chara corallina, 4M^m + 8S₁^m+ 2S₂^m for Chara vulgaris, 6M^m + 4S₁^m + 2S₂^sm +2S₂^m for Chara gymnopitys and 6L^m + 2L^sm + 6M^m+ 2M^sm + 2S₁^m for Nitella translucens.

Keywords: Chara, Nitella, Karyotype, Green algae, Bangladesh

Location: Professor Sultanul Alam Cytogenetics Laboratory, Department of Botany, University of Rajshahi

Start Date:

End Date:

Program Area: Variety and Species

Non-commodity: Performance

Objectives:

To report the chromosome number and their morphology because so far no such work has been done in Bangladesh.

Methodology:

Chara corallina Linn., Chara vulgaris Linn., Chara gymnopitys Linn. and Nitella translucens Ag. were collected from different places in Rajshahi district, Bangladesh.Antheridial filaments were the most favourable material for cytological study. Green tips with prominent sex organs were collected and fixed in 1:1 aceto-alcohol solution for 24-48 hours and then transferred to 70% ethanol. Iron-alum acetocarmine method was applied with slight modification. Sometimes for clear staining 1% haematoxylin was used instead of acetocarmine. Squashing and light tapping were carried out for better spreading of metaphase chromosomes in one plane. The number of chromosomes for each of algal species were counted and length of chromosomes with respect to the centromeric position were measured from camera lucida drawings. From the total chromatin length (TCL), TF% and coefficient of variation (CV) among the chromosome complement were computed statistically by conventional methods to characterize each of four karyotypes of the Charophytes.

Source of Information: Bangladesh J. Bot. 38(2): 205-207, 2009 (December)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The haploid chromosome numbers were found to be 42, 14, 14 and 18 in Chara corallina, C. vulgaris, C. gymnopites and Nitella translucens, respectively.

Knowledge Management: Journal