Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Screening of Pea Genotypes Tolerant to Iron Deficiency Based on Physiological Traits

Imran Ahmed
Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Ahmad Humayan Kabir
Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Mohammad Farhadur Rahman
Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Mohammad Firoz Alam
Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh

Abstract/ Executive Summary:

Screening of pea genotypes tolerant to Fe deficiency was performed in a number of Australian (Santi, Parfield, BC₁₁, BC₁₉₁, BC₁₇ and BC₁₄) and Bangladeshi (BARI-1 and IPSA-2) genotypes based on different physiological parameters. Fe deficiency caused severe decrease in chlorophyll a and b concentrations in Parafield, BC17 and IPSA-2 grown on MS (Murashige and Skoog) media on in vitro conditions. In contrast, chlorophyll a and b concentrations were not significantly decreased in Santi, BC11, BC91, BC14 and BARI-1. Furthermore, number of leaves, shoot height and weight were significantly reduced in Parafield, BC₁₇, BD₁₄ and IPSA-2; whereas Santi, BC₁₁, BC₉₁ and BARI-1 did not show prominent decrease in the aforesaid growth parameters due to Fe deficiency. Again, Parafield, BC₁₇ and IPSA-2 showed significant decrease in root length and root biomass under Fe deficiency. In contrast, these parameters were unchangeable in Santi, BC₁₁, BC₉₁, BC₁₄ and BARI-1 in Fe shortage compared to controls. Based on these findings, tolerance to Fe deficiency in these pea genotypes can be categorized as: tolerant (Santi, BC₁₁, BC₉₁, BARI-1), intermediate (BC₁₄) and sensitive (Parafield, BC₁₇, IPSA- 2). This study demonstrates the effectiveness of in vitro culture as an efficient method to screen Fe-efficient crop plants. Moreover, the ranking can be applied in plant breeding program and may have great advantage over conventional methods.

Keywords: In vitro culture, Fe deficiency tolerance, Peas, Chlorophyll concentration, Screening

Location: Department of Botany, University of Rajshahi, Rajshahi

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Pea

Objectives:

To establish in vitro method for screening Fe deficiency in pea genotypes
To explore possibility, where facilities and spaces are not available for field or hydroponic methods.

Methodology:

Seeds of six Australian genotypes (Santi, Parafield, BC₁₁, BC₁₉₁, BC ₁₇ and BC₁₄) and two Bangladeshi genotypes (BARI-1 and IPSA-2) of Pisum sativum were collected from Dr. Jeff Paull, The University of Adelaide and Bangladesh Agricultural Research Institute, respectively. Seeds were surface sterilized in 70% ethanol for 1 min and then washed in 5% sodium hypochlorite for 15 min. Seeds were then rinsed five times in sterile deionised water. Seeds were then germinated on moist filter paper wetted with deionised water placed on petridishes for one week in the dark at room temperature. After the roots started to germinate, only healthy and uniform seedlings were transferred to MS media supplemented with 1% sucrose, 0.5 g l^-1 MES and 1% agar. Two different treatments were carried out: (a) control: MS media including Fe (b) treatment: MS media excluding Fe. The pH was adjusted to 6.0 by KOH/HCl just before autoclaving the medium at 121⁰C for 20 min. Plantlets were maintained in a climatic chamber at 24⁰C, under 55μmol m^-2sec^-1 PAR of light intensity and a 16/8 light/dark photoperiod and sub-cultured every 3 weeks. Chlorophyll (a and b) concentrations were measured according to the spectrophotometric method with some modifications. Briefly, the leaf samples were harvested and immediately dried in freezer. The leaf samples (50mg) were then grinded with mortar and pestle. About 8.0 ml of 96%-ethanol was then added and homogenized using vortex. The samples were placed in test tubes wrapped by aluminium foil and let them incubate at room temperature in an exhaust hood overnight. The next day, the samples were vortexed before measuring the absorbance of the extract at 470.0 nm, 648.6 nm and 664.2 nm. The number of leaves on each plant was counted three weeks after Fe deficiency was imposed. Whole shoot and root lengths were measured for each plant sample using a ruler. For measurement of fresh weight of root, roots were harvested and then wiped with clean tissue paper before measuring weight in electronic balance. Fresh weight of shoot was directly measured after harvesting. For measuring dry weight, roots and shoots were quickly rinsed in deionised water and then wiped with clean tissue paper. Root and shoot samples were then dried in an oven at 70^oC for two days before dry weight was measured. Statistical analyses (t-test) were performed using Genstat software (14th Edition). Significance was set at P≤0.05. Three replications of each sample were used for all experiments.

Source of Information: International Journal of Biosciences | IJB |; ISSN: 2220-6655 (Print), 2222-5234 (Online); Vol. 6, No. 2, p. 460-467, 2015

Article Link (Online Journal): http://www.innspub.net; http://dx.doi.org/10.12692/ijb/6.2.460-467

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It was evident that, genotypic variation exits in both Australian and Bangladeshi genotypes in response to Fe deficiency. Taken as a whole, Santi, BC₁₁, BC₉₁and BARI-1 were highly tolerant to Fe deficiency, showing normal chlorophyll synthesis and physiological growth. BC₁₄ could be termed as intermediate genotype as this line showed both tolerance and sensitivity to Fe deficiency. Finally, Parafield, BC₁₇ and IPSA-2 were highly sensitive and unable to survive or maintain normal growth and development under Fe deficiency. Concentration of chlorophyll a in young leaves in a number of pea genotypes grown in Fe-sufficient (Fe⁺) and Fe^- deficient (Fe^-) in vitro culture. Different letters indicate significant differences between means ± SD of treatments (n = 3),

Knowledge Management: Journal