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Disease Reaction of Potato Germplasms and True Potato Seeds Against Rhizoctonia Solani Kuhn

Md. Maniruzzman Khandakar
Department of Botany, Jagannath University, Dhaka, Bangladesh

Abul Khair
Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh.

Md. Khurshed Alam Bhuiyan
Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh.

Abstract/ Executive Summary:

Screening of 25 potato germplasm against Rhizoctonia solani BTB115 in artificially infested field condition were carried out at the Department of Botany, Jagannath University, Dhaka. No germplasm was found resistant to R. solani. Out of the total, only six germplasms appeared to be moderately resistant. Cardinal, Diamant and Heera were found as highly susceptible varieties. True potato seed viz, BARI TPS1 was tested against two virulent isolates of R. solani DK64 and BTB115. Only 21% and 29% resistant seedlings against the DK64 and BTB115 isolates, respectively were obtained compared to 85% seedlings in control.

Keywords: Disease reaction , Germplasm, Potato, TPS, Rhizoctonia solani

Location: Department of Botany, Jagannath University, Dhaka

Start Date:

End Date:

Program Area: Pest Management

Commodity: Potato

Objectives:

To investigate on the resistance level of the recommended varieties and lines of potato as well as true potato seeds against R. solani.

Methodology:

To evaluate the disease responses of potato germplasms against virulent isolate of R. solani, 25 germplasms such as Adora, Arinda, Cardinal, Dheera, Diamant, Heera, Kuroda, Multa, Prelude, Raja, Patronies, 44, 37, 140, 430, 93.314, 93.316, 93.319, 87.12, 88.163, 94.42, 94.66, 384011.3, 384091.11 and 384558.10 were tested. Inocula of the fungal isolates were prepared following standard method. Fifteen mycelial agar discs of R. solani isolate BTB115 were transferred into conical flasks and were mixed with autoclaved wheat grains. The flasks were incubated at 25 ± 2º C for three weeks. After incubation the colonized wheat grains were air dried and stored at 4º C until use. Before sowing, the land was sterilized with 1% formaldehyde. Ten days later the soil of the field was inoculated with the pathogen R. solani isolate @ 94 g/m². Plot size was 0.60 m × 1 m and each plot was divided longitudinally into two rows with 0.1 m gap. In each row 5 potato seeds were sown after seven days of inoculation with isolates. Each seed tuber was cut longitudinally into two halves and air dried 24 h before planting. Recommended doses of well decomposed cowdung as well as urea, triple super phosphate and muriate of potash for N, P and K, respectively were used. Design of the experiment was RCB having three replications. Data on total number of plants and tubers, infected number of plants and tubers, ratings of percent disease index (PDI) and yield of each plot were recorded. Disease incidences were calculated with the following formula: Disease incidence = (Number of infected plants/Total number of potato plants) x 100. The PDI was appraised by indexing the visible damage of a given plant on a six degree rating scale from 0 (no disease symptom) to 5 (plant completely girdled/ more than 80% or whole of the tuber infected which may be cracked and deformed). The PDI was calculated with the following formula: PDI = [Summation of all ratings/ {Number of plants/tubers observed × maximum rating (5)}] x 100. Responses of true potato seeds BARI TPS1 were assessed against R. solani BTB115 and DK64. Steam sterilized soil was inoculated with the isolates @ 10g inocula in per kg of soil separately. Then inoculated soil was poured in plastic trays and incubated for seven days. Steam sterilized soil without inoculation with R. solani isolate served as control. One hundred seeds were sown in each tray having three replications for each treatment. Data on seedling emergence and seedling survival were recorded 20, 30 and 40 days after seed sowing.

Source of Information: Bangladesh J. Bot. 40(2): 193-196, 2011 (December)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Upon study of the Disease reaction of the potato germplasms and True Potato Seeds, only 21% and 29% resistant seedlings against the DK64 and BTB115 isolates, respectively were obtained compared to 85% seedlings in control.

Knowledge Management: Journal