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Propagation of Papaya (carica Papaya L.) Cv. Shahi Through in Vitro Culture

PROTUL KUMAR ROY
Institute of Food and Radiation Biology, Atomic Energy Research Establishment, G.P.O Box 3787, Dhaka-1000, Bangladesh

SHYAMAL KUMAR ROY
Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh.

MD LOKMAN HAKIM
Institute of Food and Radiation Biology, Atomic Energy Research Establishment, G.P.O Box 3787, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

A large number of shoots regenerated from lateral buds and young leaves of Carica papaya L. cv. Shahi on MS supplemented with 1.0 mg/l zeatin and 0.2 mg/l NAA. Addition of 200 mg/l casein hydrolysate (CH) to the medium increased the number of shoots per culture and incorporation of 2.0 g/l activated charcoal (AC) to the medium resulted effective shoot growth with healthy leaf. While addition of 100 mg/l urea and 2.0 g/l activated charcoal to the medium showed proper shoot elongation. Best rooting was obtained from shoots cultured on half-strength of MS fortified with 4.0 mg/l IBA.Within four weeks of transfer to the rooting medium, 90% microcuttings produced 12 - 14 roots. The regenerated plantlets were successfully transferred to potted soil. About 84% plantlets survived in the experimental field.

Keywords: In vitro culture, Explants, Regeneration, Carica papaya

Location: Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Papaya

Objectives:

To develop protocol for propagation of papaya through in-vitro culture.

Methodology:

Biologically papaya has three types such as male, female and bisexual but only female and bisexual types are productive. In commercial plantation it is very often found that male plants prevail as high as 30% and some times over 50% of the total. Conventional method of propagation like cutting or grafting has not been found successful in papaya. In this regard clonal propagation represents the economic way of continuously producing new uniform true-to-parental type planting materials of known superior lines. Cloning by in vitro technique has been proved as an excellent biotechnology of vegetative propagation specially for those, which are difficult to rooting. In vitro micropropagation system of papaya was reported by many researchers. But their reports were not satisfactory for large scale propagation of plantlets as well as successful field transfer. Thus considering the above facts, the present study was undertaken to develop a suitable protocol for in vitro propagation of papaya. Young plants of Carica papaya L. cv. Shahi were collected from local nursery and planted in the research field of the Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka for the collection of explants for present investigation. Plant materials, namely lateral buds and young leaves were collected in a beaker containing tap water to avoid desiccation. Surface sterilization was done under aseptic conditions in laminar air flow cabinet. They were surface sterilized with 70% ethyl alcohol (1 min) followed by 0.1% HgCl₂ for 8 min. After surface sterilization, lateral buds and young leaves were divided into small pieces (approx. 1.0 - 1.5 cm). These were used as explants and cultured onto culture medium.

Source of Information: Bangladesh J. Bot. 41(2): 191-195, 2012 (December)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present work demonstrated a protocol for in vitro propagation of Carica papaya L. cv. Shahi.

Knowledge Management: Journal