Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Proteolytic Activity, Growth and Nutrient Release by Bacillus Cereus Lw-17

Md. Abdul karim
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Nazneen Akhter
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Sirazul Hoque
Department of Soil, Water and Environment, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

Water pollution is now a major area of concern in Bangladesh. A study thus was undertaken to find out proteolytic activity, growth and nutrient release by release by bacteria which could act as a natural agent to reduce pollution in water. A total of 85 fast growing bacterial colonies were isolated from surface water in September 2009 from the Gulshan lake, Dhaka.It was observed that, Bacillus cereus LW-17 enhanced net regeneration of dissolved inorganic nitrogen and phosphorus in the Gulshan lake water by about two-folds at temperatures of 26°C and 30ºC, respectively. Application of Bacillus cereus LW-17 would be promising for improvement of polluted environment.

Keywords: Proteolytic acitivity, Bacillus cereus LW-17, Lake water, Nutrient release

Location: Gulshan lake, Dhaka

Start Date: 00-00-2009

End Date: 00-00-2009

Program Area: Pest Management

Commodity: Diseases

Objectives:

To find out proteolytic activity, growth and nutrient release by Bacillus cerus LW-17.

Methodology:

A total of 85 fast growing bacterial colonies were isolated from surface water in September 2009. Out of them isolate LW-17 was selected for biotechnological application depending on growth pattern and proteolytic activities. Bacterial re-suspension was prepared as described by Karim et al. 2003. Five ml of bacterial cell re-suspension was used as inoculum and it was inoculated into the treatment glass bottles (Schott, Duran, 250 ml, Germany). The glass bottles were incubated in a light condition (in duplicate) at 26ºC temperature during September 2010 and at 30ºC during October 2010 for 15 days without any shaking. Bacterial activities were evaluated measuring the changes of inorganic nutrient concentrations into the water during incubation with or without adding bacteria. At five days interval two glass bottles were taken for chemical analysis. Water samples were passed through the filter paper (Whatman No. 42, England) to eliminate suspended solid particles. The concentrations of inorganic form of nitrogen (DIN: ammonium, nitrate and nitrite) and phosphorus (DIP) in the experimental glass bottles were measured. Water-soluble NO₂ -N was determined by modified Griess-Ilosvay method and water-soluble NO₃-N was determined colorimetrically. Ammonium content of the samples was determined by Micro-Kjeldahl’s method. Soluble reactive phosphorus content of the water samples was determined by ascorbic acid blue color method.

Source of Information: Bangladesh J. Bot. 42(2): 349-353, 2013 (December)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The Bacillus cereus LW-17 was found promising in reducing water pollution of the lake water

Knowledge Management: Journal