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Effects of Nacl Stress on Accumulation of K+, Na+, Cl-, No3 -, Sugar and Proline Contents in the Seedlings of Triticale-i

RIFAT SAMAD
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

JL Karmooker
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

NaCl stress at 50 -150 mM NaCl caused a 12 - 16 fold increase in Na+ accumulation in the root and 3 - 10 fold in the shoot of Triticale-I with a concomitant decrease in that of K+ and Cl-. NaCl stress increased the accumulation of reducing, total sugars and proline both in the root and shoot of the seedlings. The mechanism of salt tolerance of Triticale-I plants with respect to ionic relation and accumulation of sugars and proline were discussed.

Keywords: Salinity, Ion transport, Sugar, Proline, Triticale

Location: Department of Botany, University of Dhaka, Dhaka-1000

Start Date:

End Date:

Program Area: Risk Management in Agriculture

Commodity: Triticale

Objectives:

To study the effect of NaCl salinity stress on the accumulation and distribution of K+, Na+, Cl-, NO₃ - in the seedlings of Triticale

Methodology:

Triticale (Triticosecale Wittmack), a hybrid between wheat and rye is tolerant to environmental stress and offers an important potential protein source for both human consumption and animal feed. Triticale seeds were collected through the courtesy of Bangladesh Agriculture Research Institute (BARI), Gazipur. Plants were grown in water culture to study the accumulation of ions (Na+, K+, Cl- and NO₃-), reducing and total sugar and proline. Seeds were surface sterilized. Then the seeds were spread over a cotton gauge placed> in a lid having nine holes (3 cm dia.) and the lid with seeds was placed on a bucket containing 5 liter of modified half-strength Hoagland solution. The bucket was painted black to avoid the exposure of light to the roots. The buckets were kept in dark for 48 h to facilitate the germination of seeds. After germination, the buckets with the seedlings were placed in a light bank at a day/night temperature of 25ºC ± 1ºC/18ºC ± 1ºC and day/night length of 14 h/10 h and light intensity was 160 μE/m²/sec. Relative humidity was 75% by day and 85% at night. Half-strength Hoagland solution was used as control. The salinity treatments were made by using 50, 100 and 150 mM NaCl solutions in half-strength Hoagland solution. Solutions of control and treatments were aerated continuously by an air compressor. After 3 days, seedlings were plugged to each hole of the lid with spongy foam for rendering support. Nutrient solution was completely replaced every 7 days. Distribution of ions was measured in roots and shoots of the seedlings after 7, 14 and 21 days of salinity treatments. Three replicates were used in each treatment. The tissues were dried in an oven at 80^oC for 72 h to attain constant weight. Na+, K+ and Cl- were extracted from dry tissue by boiling in water bath with two changes of 10 ml distilled water contained in test tubes. Na+, K+ and Cl- and NO3 - contents were measured. Proline was measured following standard method. Reducing and total sugars were extracted by boiling fresh root and shoot tissue in two changes of 5 ml of 80% ethanol for five minutes in a hot water bath and measured following Somogyi-Nelson method.

Source of Information: Bangladesh J. Bot. 42(2): 189-194, 2013 (December)

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Salinity- induced dramatic stimulation in accumulation of sugars and proline in Triticale-I which helped to maintain the osmotic potential of cytoplasm of cells, so important for the survival of plants under stress.

Knowledge Management: Journal