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Crystal Formation in Cultures of Fungi: New Records from Bangladesh

Shamim Shamsi
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

Najmum Naher
Department of Botany, Life and Earth Science Group, National University, Gazipur-1704, Bangladesh.

Yasmin Fatema
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

In the present study crystal formations were detected in the culture of Aspergillus niger, A. fluvus, Aspergillus sp., Bipolaris sorokiniana, Cladosporium spp., Colletotrichum gloeosporioides, Colletotrichum orbiculare, Curvularia spp., Fusarium sp., Paecilomyces spp., Penicillium sp., Pestalotia sp., Pestalotiopsis guepinii, Phaeoisariopsis personata, Trichoderma viride and seven sterile culture of fungi. Eighteen types of crystallographic structures were detected from different fungal isolates. This is the first report of formation of crystal in fungal cultures from Bangladesh.

Keywords: Crystal, Culture, Fungi, Bangladesh

Location: Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

Start Date: 00-07-2009

End Date: 00-05-2014

Program Area: Pest Management

Commodity: Diseases

Objectives:

To record the different types of crystal forms as they occur in fungal cultures.

Methodology:

The fungi were isolated from healthy and diseased plant parts following "Tissue planting" and "Blotter" methods. Some air borne fungi isolated on Potato Dextrose Agar (PDA) plate were found to produce crystals in cultures at 25-28 ºC and pH 6, within 7 days of incubation. Sporulating fungal cultures showed crystal in slide. Fungal structure like mycelia, spore bearing structures and spores were scrapped of from the surface of the sporulating colony with a scalpel or blade or picked up with a needle and was mounted in lacto phenol over a clean slide for microscopic observation. In case of hyaline structures, a little amount of aniline blue (cotton blue) was added to the mounted fluid. Crystals may be found as early as the seventh day and older culture contain more crystals. Crystals ware observed with fungal structures under Binocular microscope with normal light. Under microscopic view different types of crystals were observed in different fungal species. The isolated fungi were identified based on morphological characteristics observed under a compound microscope following standard keys.

Source of Information: J. Asiat. Soc. Bangladesh, Sci. 41 (I): 59-66, June 2015,1016-6947

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Eighteen types of crystallographic structures were detected from different fungal isolates. This is the first report of formation of crystal in fungal cultures from Bangladesh.

Knowledge Management: Journal