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Comparative Analysis of Haemagglutination Inhibition Antibody Production Against Three Lentogenic Newcastle Disease Virus Vaccines in Broiler Chicks

N C Shil
Additional Veterinary Surgeon, FAO Technical Unit, Bird Flu Active Survillance Programme, Food and Agriculture Organization, Dhanmondi-R/A, Dhaka-1205, Bangladesh

M S Mahmud
Department of Microbiology, Faculty of Heath Sciences, Gono Bishwabidyalay (University), Savar, Dhaka-1344, Bangladesh

M M Amin
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202 , Bangladesh

M Anwar Hossain
Department of Microbiology University of Dhaka, Dhaka 1000, Bangladesh

Abstract/ Executive Summary:

The comparative analysis of haemagglutination Inhibitation (HI)-antibody production against three lentogenic Newcastle disease virus vaccines BCRDV (F-strain), Izovac B1 Hitchner ® (B1 strain) and Cevac New L® (LaSota strain) was analyzed in broiler chicks. For this, three groups A, B and C, each consisted of 30 birds were vaccinated with single dosage at day 9 and the other group of 10 birds was maintained as unvaccinated control. Sera samples obtained randomly from 10 birds of each group on the day 17, 20, 28 and 32 and the HI titers estimated. Maternally derived antibody persisted to a maximum level of 17 day and than declined to minimum or none. The results revealed that vaccination of young birds with locally produced BCRDV by the Department of Livestock Service elevated HI antibody at comparable level with that of commercially available vaccine Izovac B1 Hitchner and Cevac New L®. The ‘F’ value preformed with mean±SD of 17, 20, 28 and 32 days appeared non significant among the HI titers of the birds vaccinated with BCRDV, Izovac B1 Hitchner ® (B1 strain) and Cevac New L®.

Keywords: Maternally derived antibody (MDA); Newcastle disease (ND); Newcastle disease virus (NDV); Haemagglutination inhibition (HI); vaccines

Location: FAO Technical Unit, Bird Flu Active Survillance Programme, Food and Agriculture Organization, Dhanmondi-R/A

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

To observe the persistence of maternally derived antibody (MDA) to NDV in chickens and to evaluate antibody production following single vaccination with BCRDV, Izovac B1 Hitchner ® and Cevac New L® in broiler chicks and also to compare the efficiency of BCRDV with those of two commercial ND vaccines i.e. Izovac B1Hitchner ® and Cevac New L®.

Methodology:

Prior to vaccination, blood samples were collected from broilers to measure maternal antibody on day 1 and 9. Three groups of A, B and C, each consisted of 30 birds were vaccinated with single dosage at day 9 and another group of 10 birds maintained as unvaccinated control. Elevation of HI titers indicated immune response in chickens after intraocular route (eye-drop) vaccination. MDA persistently maintained in vaccinated broiler chickens up to 20 days old and then decline to minimum or none. Moreover, in control group D, high level of HI titers were found during first two weeks of life which correlated the findings reported elsewhere. The immune response is improved in elder chicks as levels of MDA fall and immunological maturity develop

Source of Information: Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 41-43

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Vaccination of young birds with BCRDV produced by DLS shows elevated HI antibody at comparable level with that of commercially available vaccine Izovac B1 Hitchner and Cevac New L®, though Cevac New L® produced the highest level of antibody among the three.

Knowledge Management: Journal