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Seroprevalence of Canine Brucellosis in Dhaka City Corporation Area, Bangladesh

M. Shamshur Rahman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

S. M. Lutful Kabir
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. Siddiqur Rahman
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

An investigation was conducted to determine the seroprevalence of brucellosis in pet dogs of Dhaka City Corporation area. A total of 50 sera samples from pet dogs were collected. Additional data on risk factors (breed, age, sex, any abnormality during sample collection etc.) were recorded during sample collection by using standard questionnaire. Sera were separated from blood samples and tested with the Rose Bengal Plate Test (RBPT) and Indirect Enzyme linked Immunosorbent Assay (IELISA) parallely. The overall seroprevalence of brucellosis in pet dogs was found 4.00% by RBPT & IELISA. The results of both serological methods were equal in terms of ability to detect Canine Brucellosis. Statistically higher seroprevalence of brucellosis (6.06%) was found in dog aged 1.5 to 2.5 years. Higher seroprevalence (15.38%) was found also in female pet dogs and no response in male pet dogs.

Keywords: Sero-prevalence; Brucellosis; Pet dogs; RBPT; IELISA

Location: Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

Start Date: 00-01-2014

End Date: 00-06-2014

Program Area: Pest Management

Commodity: Diseases

Objectives:

To determine the seroprevalence of brucellosis in pet dogs of Dhaka City Corporation area, Bangladesh.

Methodology:

For serological study a total of 50 pet dog’s serum samples were collected during the period of January-June, 2014 from Central Veterinary Hospital, 48, Kazi Alauddin Road, Dhaka-1000. The study recorded some clinical, epidemiological and reproductive data in the sample collection sheet including species, breed, age, sex, any genital lesion during sample collection etc. The animals were controlled by mechanical device and about 3-5 ml of blood was drawn directly from the fore arm with the help of sterile syringe and needle. During collection of blood all precaution were taken to avoid hemolysis. The collected blood was immediately transferred to the collecting test tubes and allowed to clot undisturbed at the room temperature for half an hour in the laboratory and later were kept overnight at 4 °C in refrigerator. Then the sera were separated in the next day by centrifugation at 3000 rpm for 10 minutes and the sera were stored at - 20 °C until tested. Each of the collected serum samples was tested with Rose Bengal Plate Test (RBPT) and Indirect Enzyme linked Immunosorbent Assay (IELISA) as per the manufacturer’s instruction. The RBPT antigen (V2001) obtained from the Manufacturer Company named Lillidale Diagnostics, Badbury View, Bothenwod, Wimborne, Dorset, BH21 4HU, UK, prepared from B. abortus (Strain 99) stained with Rose Bengal dye and suspended in acid buffer (pH 3.65). Test results and potential association with age, sex and among tests were performed by SPSS 11.5 for Chi-square analysis.

Source of Information: Asian J. Med. Biol. Res. 2015, 1 (1), 17-21; ISSN 2411-4472

Article Link (Online Journal): www.ebupress.com/journal/ajmbr

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The result of this study would provide a baseline data for further study on canine brucellosis in dog population. Pet dog may be considered as a carrier of Brucella abortus infection and indicate a risk (source) for human and food animals. This study suggests that the findings of RBPT and IELISA were same. The finding of this study is suggested also that dogs should be routinely included in the brucellosis surveillance and control program in human and food animals.

Knowledge Management: Journal