Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Xanthone from the Fruits of Terminalia Arjuna

R. Hossain*
Department of Chemistry, Chittagong University of Engineering & Technology, Chittagong 4349, Bangladesh

R. Sultana
H.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi 75270, Pakistan

M. I. Choudhary
H.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi 75270, Pakistan

S. Zaman
Department of Chemistry, University of Rajshahi, Rajshahi 6205, Bangladesh

Abstract/ Executive Summary:

2,7-dimethoxyxanthone (1) along with a well known compound β-sitosterol-3-O-β-Dglucopyranoside (2) were isolated from the methonalic extract of the fruits of Terminalia arjuna. Their structures were elucidated by spectroscopic (NMR and mass) analysis.

Keywords: Terminalia arjuna; Combretaceae; 2,7-Dimethoxyxanthone.

Location: University of Rajshahi, Bangladesh

Start Date: 00-09-2007

End Date:

Program Area: Resource Development and Management

Non-commodity: Performance

Objectives:

Objective of the study was to isolate one xanthone identified as 2,7- dimethoxyxanthone (1).

Methodology:

NMR Spectra: Bruker spectrometer ( 1H 600 & 500 MHz and 13C 150 & 100 MHz) and residual protons used as internal standard. EI mass: JEOL (JMS 600H) mass spectroscopy. IR: SIMADZU FTIR (8900). UV: HITACHI (U-3200) spectrophotometer. Optical rotation: JASCO (P-2000) polarimeter. Melting point: BUCHI 535 digital Melting Point apparatus and were uncorrected. TLC spots were detected by UV light and fixed upon spraying ceric sulfate reagent followed by heating. The fruits of Terminalia arjuna W. & A. were collected from the campus of University of Rajshahi, Bangladesh, during September 2007 and was authenticated by Bangladesh National Herbarium, Dhaka, Bangladesh. A voucher specimen has been deposited (Acc. No. 32612, DACB) in the Bangladesh National Herbarium, Dhaka, Bangladesh.

Air dried fruits of T. arjuna was powdered (18 kg) and extracted with methanol (MeOH) (60 Lx3) at room temperature for 3 days. MeOH was evaporated under vacuum to give a solid product (850 g), which was dissolved again in MeOH and partitioned with n-hexane to remove sticky and gummy materials. MeOH was evaporated under vacuum to give a residue (590 g). 200 g from this residue was suspended in H₂O and partitioned with dichloromethane (5 Lx3), ethyl acetate (5 Lx3) and n-butanol (5 Lx3) successively. The Ethyl acetate fraction (E, 14.5 g) was subjected to column chromatography over silica gel (30x7 cm) eluted with ethyl acetate : n-hexane and the fractions were collected at 20% ethyl acetate (E-1), 50% ethyl acetate (E-2), 75% ethyl acetate (E-3), 100% ethyl acetate (E-4) and 100% methanol (E-5). Fraction E–1 (0.6 g) was subjected to column chromatography with silica gel (25x2.5 cm) and eluted with 20% ethyl acetate : n-hexane to give a mixture which on further purification by preparative TLC over silica gel with 40% acetone : n-hexane as eluent gave a compound 2,7-dimethoxyxanthone (1) (2.8 mg). Known compound β-sitosterol-3-O-β-D-glucopuranoside (2) (21.4 mg) was obtained from fraction E–3 by reversed-phase C18 silica gel and Sephadex LH 20 column chromatography.

Source of Information: J. Sci. Res. 8 (1), 49-54 (2016)

Article Link (Online Journal): http://www.banglajol.info/index.php/JSR/article/view/23482/17508

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Synthesized compound 1 was isolated from the methanolic extract of the fruits of Terminalia arjuna and the structure was identified as 2,7-dimthoxyxanthone which was isolated first time from any natural source, confirmed by SciFinder . Compound 2 showed the physical and spectral data similar to the reported.

Knowledge Management: Journal