Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Potential of Cypermethrin or Diazinon and Their Combined Action with Piperonyl Butoxide (pbo) Against Periplaneta Americana L

A S. M .Shafiqur Rahman
Department of zoology, University of Rajshahi, Rajshahi 6205, Bangladesh

M.Y. Akter
Department of zoology, University of Rajshahi, Rajshahi 6205, Bangladesh

Abstract/ Executive Summary:

Initial bioassays were carried out to establish the effect of single treatment. It was observed that cypermethrin was more toxic than diazinon. The topical LD₅₀ values of diazinon was 511.55 ng/insect where as it was recorded as 313.04 ng/insect in cypermethrin. Combining PBO both diazinon and cypermethrin indicated a synergistic action at 1:5 ratio followed by 1:2 and 1:1 ratios. The cotoxicity coefficient values also indicated that there was an increase in synergism with increase PBO ratios. The isobole for mixtures of both test insecticides with PBO are of similar shape indicating that a similar response was talking place. The effect of PBO to insects has been studied by several investigators. It is now generally recognized that PBO produces to synergistic effect by inhibiting the detoxifying enzymes within insect body.

Keywords: Periplaneta americana, diazinon, cypermethrin, LD50, synergist

Location: Department of zoology, University of Rajshahi, Rajshahi

Start Date:

End Date:

Program Area: Pest Management

Commodity: Insecticide

Objectives:

The present paper describes the effect of diazinon and cypermethrin and their combined action with a known synergist PBO against the American cockroach, P. americana under laboratory condition.

Methodology:

Bioassays were carried out by micro topical application using a Hamilton micro syringe (ml) with canulae (G 36X3) using the standard procedure. A micro-syringe calibration using mercury was done before the tests. Each bioassay included a minimum of five concentrations of insecticides and an acetone treated control. The mixture of inscticides and piperonyl butoxide (PBO) were used in different ratios( viz.1:1,1:2 and 1:5). Five-day-old adult cockroaches were used for bioassay. Treatment was made by applying 1.0 μl solution of test insecticides to the dorso-thoracic region of individual insect under anesthetic condition with five replications. After treatment the insects were placed in glass beakers (500 ml.) containing a small amount of food in an incubator at 30º C.

Source of Information: Univ. j. zool. Rajshahi Univ. Vol. 27, 2008 pp. 101-102; ISSN 1023-6104

Article Link (Online Journal): http://journals.sfu.ca/bd/index.php/UJZRU

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In the present investigation PBO increased the toxicity of both diazinon and cypermethrin against P. americana. This result therefore, suggests that inhibition of oxidative detoxification might be involved to some extent.

Knowledge Management: Journal