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Prevalence of Bovine Tuberculosis in Cattle in the Selected Upazila of Sirajganj District in Bangladesh

M. A. A. Mahmud
Additional Veterinary Surgeon, Upazila Livestock Office, Ullapara, Sirajganj

S. M. S. H. Belal
Veterinary Surgeon, District Veterinary Hospital, Sirajganj,

N. Z. Shoshe
Department of Livestock Production and Management, Sylhet Agricultural University, Sylhet, Bangladesh

Abstract/ Executive Summary:

A study was conducted to ascertain the prevalence of Bovine tuberculosis at 135 commercial dairy farms of five upazilas of Sirajganj district of Bangladesh during the period from January 2012 to December 2013. During two years study period a total of 270 blood samples were tested by Antigen Rapid Bovine TB Ab test kit. The overall prevalence of bovine tuberculosis was recorded as 7.78%. Distribution on the basis of age, sex and breed revealed the significant relationship among the infection, breed, sex and age in the population. The prevalence was relatively high in older cattle (10.23%) and the highest prevalence was observed in the female (9.09%). The prevalence of tuberculosis infection was considerably high in most of large herds. It was evident from the study that cattle were infected with organism and caused a serious economic loss.

Keywords: Prevalence, Bovine tuberculosis, Antigen Test Kit

Location: Sirajganj Sadar, Shahjadpur, Ullapara, Kamarkhand and Belkuchi of Sirajganj district

Start Date: 00-01-2012

End Date: 00-12-2013

Program Area: Animal Health and Management

Commodity: Diseases, Cattle

Objectives:

To know the prevalence of bovine tuberculosis in cattle of Sirajganj district as measured by the Antigen Rapid Bovine TB AbTest Kit.

Methodology:

Location and duration of study: Five upazilas of Sirajganj district namely Sirajganj Sadar, Shahjadpur, Ullapara, Kamarkhand and Belkuchi were selected on the basis of number and size the herds. Three administrative union from each upazila and three villages from each union were taken for this study. The study was conducted over a period of 24 months during January 2012 to December 2013.
Animal selection and sample collection: Three herds from each village and two cattle of over 6 months old from each herd were selected for the study. About 10 ml of whole blood sample was collected from the jugular vein, using plain vaccutainer tubes and needles from each cattle aged above six and with no history of vaccination for tuberculosis. Each sample tube was labeled using codes specific to the individual sample. The tubes were tilted and serum was collected either passively by decanting or after centrifugation of the blood samples at 2,500 rpm for 5 minutes. The serum was stored at −20°C until tested serologically.
Rapid kit test (Immuno chromatographic assay): The samples were subjected to Antigen Rapid Bovine tuberculosis Antibody Test Kit (Immuno chromatographic assay) for the detection of TB antibodies. For this, Ten μl (one drop) serum was added to the sample hole marked “S” on the test device with a capillary tube or single cannel micro titer pipette and waited for 1 minute and then added 3 drops of the developing buffer into the developing buffer hole. Appearance of a purple band in the result window of the test kit within 20 minutes indicates positive result and indicating control band only in negative result. In positive cases, intensity of the faint purple band appeared within 20 minutes. No result was read after 20 minutes.
Statistical analysis: Data were entered in Microsoft Excel 2007 and transferred to R 2.14.2 (The R Foundation for Statistical Computing, Vienna, Austria). Descriptive statistics were obtained using Data Mining package of the software R 2.14.2.

Source of Information: Bangl. J. Vet. Med. (2014). 12 (2): 141 - 145 ISSN: 1729 - 7893 (Print), 2308 - 0922 (Online)

Article Link (Online Journal): http://www.banglajol.info/index.php/BJVM/article/view/21276/14604

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In conclusion, therefore, for the control and eradication of tuberculosis in cattle population, more attention should be paid towards separation of healthy and infected animals/herds. This should be combined with more government intervention in the areas of regulations and policies concerning routine screening of all cattle populations. Awareness program should be undertaken involving stakeholders in the livestock industry as well as consumers to avert public health, veterinary public health and economic losses associated with tuberculosis.

Knowledge Management: Journal