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Quality Deterioration of Mass Reared Offsprings of Bracon Hebetor in Successive Generations

M. S. Alam
Vertebrate Pest Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh

M. Z. Alam
Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

S. N. Alam
Entomology Division, Bangladesh Agricultural Research Institute, Gazipur, Banglades

M. R. U. Miah
Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

M. I. H. Mian
Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

M. M. Hossain
Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

Abstract/ Executive Summary:

A study was undertaken to determine the generation-wise parasitizing efficiency of mass reared Bracon hebetor on wax moth (Galleria mellonella). Two experiments were conducted to achieve the objectives. In the first experiment, the host larvae and parasitoid were released in the field micro plot during first generation and reared in the laboratory in subsequent generations. In another experiment, all generations were developed in the laboratory. In the first experiment, generation showed significant variation on sex ratio of adult progeny production. The male sex ratio increased with the range of 62.89 to 74.58% and female sex ratio declined ranging from 39 to 25% with the advancement of generations. The percent parasitization and adult progeny production was not influenced appreciably with the progress of generation. In the second experiment parasitization and adult progeny production did not vary significantly in successive generations. However t here was a decreasing trend in parasitization from 95 to 55% by Bracon hebetor and adult progeny production by 47.5 to 15.5 adults female-110 larvae-1 over generation. The sex ratio of adult progeny was significantly influenced by inbreed generation in laboratory reared population. Female de crease in sex ratio from first to sixth generation was 39.25 to 16.88%.

Keywords: Bracon hebetor, Inbreeding, Quality, Fitness

Location: Entomology Division, BARI, Gazipur

Start Date: 00-02-2011

End Date: 00-07-2011

Program Area: Pest Management

Commodity: Insects, Brinjal

Objectives:

To determine the generation wise parasitic efficiency of mass reared B. hebetor to maintain the quality of the laboratory population for effective biological control program.

Methodology:

The study was undertaken in the micro plot of brinjal field and in the laboratory of Entomology Division, BARI, Gazipur during February 2011 to July 2011. Two experiments were conducted.

Parasitization of host larvae in the field and subsequently in the laboratory: Ten host larvae of wax moth, Galleria mellonella were released on the plant in the caged micro plot (1.5 m x 1 m) of brinjal field. Three brinjal plants were grown in each micro plot. The plants were then encircled with 32 mesh nylon net. Ten Bracon hebetor adult (6 female and 4 male) were then released on the plant in the caged micro plot. After 2-3 days the parasitized larvae were collected from the field and kept in the petridishes. The numbers of parasitoid adults were recorded and kept in test tube (10 cm x 2 cm) containing honey solution. The successive generations (up to 7 generations) were continued in the laboratory. Ten last instars (5-6 instar) wax moth, Galleria mellonella L. larvae were released in a plastic jar (1000 ml) having a corrugated paper sheet. The full-fed larvae took position on the corrugated paper sheet for pupation. T wo B. hebetor adults (1 male and 1 female) emerged from field collected paralyzed G. mellonella were released in the plastic jar with honey solution on the cotton ball for their food. The mouth of the plastic jar was closed with black cloth. The plastic jars with wax moth larvae and B. hebetor were kept on a rack in the laboratory for 8-10 days for parasitization, egg laying, pupation and emergence of adult B. hebetor. The experiment was conducted four times. Data on generation-wise parasitization, total adults emergence per female and sex ratio were recorded carefully.

Parasitization of host larvae in the laboratory: Ten last instar (5-6 instar) wax moth, Galleria mellonella L. larvae were released in a plastic jar (1000 ml) having a corrugated paper sheet inside. The full-fed larvae took position on the corrugated paper sheet within the plastic jar for pupation. One pair laboratory reared adults of B. hebetor (1 male and 1 female) were released in the plastic jar having honey solution soaked in cotton ball for their food. The mouth of the jar was closed with black cloth. The jar containing wax mot h larvae and B. hebetor were placed on a rack in the laboratory for 8-10 days for parasitization, egg laying, pupation and adult emergence of parasitoid. The experiment was continued for successive 6 generations. Data on generation-wise parasitization, total adults per female and sex ratio were recorded.

Data analysis: Data on parasitism, offspring production and sex ratio were analyzed statistically using one - way analysis of variance (ANOVA) following SPSS 16 software and means were compared following Tukey,s Honestly Significant Different Test. The percent data were transformed to arcsine before analysis. Relationship of percent parasitization, adult progeny production and sex ratio with generation were measured by regression analysis.

Source of Information: SAARC J. Agri., 13 (2): 1-12 (2015)

Article Link (Online Journal): http://www.banglajol.info/index.php/SJA/article/view/26564/17815

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

There was a decreasing trend in parasitization from 95 to 55% by B. hebetor and adult progeny production by 47.5 to 15.5 adults female-110 larvae-1 over generation in laboratory reared population. The sex ratio of adult progeny had been significantly influenced by inbreed generation in laboratory reared population. Female decrease in sex ratio from first to sixth generation was 39.25 to 16.88%.

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