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Establishment of Ovum Pick Up (opu) Protocol for Multiplication of Red Chittagong Cattle

G. K. Deb
Bio-technology Division, Bangladesh Livestock Research Institute, Savar, Dhaka.

M. F. Afroz
Bio-technology Division, Bangladesh Livestock Research Institute, Savar, Dhaka.

M. A. Kabir
Bio-technology Division, Bangladesh Livestock Research Institute, Savar, Dhaka.

T. N. Nahar
Bio-technology Division, Bangladesh Livestock Research Institute, Savar, Dhaka.

M. K. Mostofa
Upazilla Livestock Officer
Department of Livestock Services, Savar, Dhaka, Bangladesh.

Abstract/ Executive Summary:

This research program was designed to generate baseline information on follicular statistics of Red Chittagong Cattle (RCC) and to establish an oocyte aspiration protocol from donor cow. Ten heifers ten regular breeder RCC cows were selected randomly from BLRl Research Herd. The ovary was grasped by inserting left hand through the rectum and the follicles were visualized by inserting a sectorial probe through the vagina. Before inserting the probe through vagina, it was disinfected by swabbing with 70% alcohol. Ovarian follicles were visualized using ultrasonography machine. Follicle numbers were recorded by counting on the screen of ultasonography machine. The diameters of follicles were recorded by measuring length and breadth of each follicle on the screen. All visible follicles (>2.0mm) were counted and graded as small «3.0mm), medium (3.0 to 8.0mm) and large (>8.0mm). The follicles were measured 3 times at a 3-day interval period without considering the stage of reproductive cycle of the experimental animals. During this experiment, a total of 137 follicles (66 Heifers and 71 in cows) were observed from 10 heifers and 10 cows. The corpus luteum was observed Heifher in the left or right ovary of 25.0% heifers and 35.0% cows. In heifer, 40.91, 45.45 and 13.64% the studied follicles were belonged to small, medium and large groups, respectively. The percentage small, medium and large follicles in the cow ovaries were 54.93, 39.44 and 5.63% accordingly. number of follicles in an ovary did not vary (P>0.05) between right and left ovary of a heifer or CI The diameter of the largest follicle of each ovary was recorded during this study. The diameter smaller (P<0.05) in the heifer (9.43±0.34mm) compared to the cows (11.2±0.73mm). The experiment on standardization of oocytes aspiration protocol from donor cows is going on. In conclusion, present study developed base line information on ovarian follicular statistics of RCC heifers and co' This information will be helpful during aspiration of ovarian follicle from donor cows.

Keywords: Follicle, Ovary, Red Chittagong cow, Oocyte aspiration, Ultrasonography

Location: Bangladesh Livestock Research Institute, Savar, Dhaka

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Cattle

Objectives:

To generate baseline information on follicular statistics of RCC and to extablish an oocyte aspiration protocol from donor cow.

Methodology:

Ten heifers and ten regular breeder RCC cows were selected randomly from BLRI Research Herd. The average body weight of the selected animals was 139.03±0.54 kg for heifers and 151.07±0.23 kg for cows. To develop expertise on manipulation of ovarian follicular statistics, initially four culled RCC cows were routinely evaluated by rectal palpation for couple of months. Later ovarian follicles were visualized by ultrasonography machine. Before visualization of ovarian follicles, the feces were removed from the rectum and the surrounding areas of vulva and rectum were swabbed with 70% alcohol to prevent infection in the reproductive tract. The ovary was grasped by inserting left hand through the rectum and the follicles were visualized by inserting a sectorial probe through the vagina. Before inserting the probe, it was disinfected by swabbing with 70% alcohol. Follicle numbers were recorded by counting on the screen of ultasonography machine. The diameters of follicles were recorded by measuring length and breadth of each follicle on the screen. All visible follicles (>2.0 mm) were counted and graded as small (<3.0 mm), medium (3.0 to 8.0 mm) and large (>8.0 mm). The machine only can measure follicles of greater than 2.0 mm. The follicles were measured 3 times at a 3-day interval period without considering the stage of the reproductive cycle of the experimental animals. The statistical analysis was done using 'SPSS-11.5' statistical package.

Source of Information: Proceedings of the Annual Research Review Workshop 2013-2014, BLRI, Savar, Dhaka. Publication No.261, pp: 68-74, October-2015.

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study developed base line information on ovarian follicular statistics of RCC heifers and cows. This information will be helpful during aspiration of oocytes from donor cows.

Knowledge Management: Report/Proceedings