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First Record on Botrytis Blight (botrytis Gladiolorum) of Gladiolus from Bangladesh

S. S. Siddique
Plant Pathology Division, Bangladesh Agricultural Research Institute (BARI), RARS, Jessore

A. U. Ahmed
Plant Pathology Division, BARI

M. S. Akter
Plant Pathology Division, Bangladesh Agricultural Research Institute (BARI), RARS, Jessore

M. M. Islam
Plant Pathology Division, Bangladesh Agricultural Research Institute (BARI), RARS, Jessore

I. H. Mian
Department of Plant Pathology, Bangabandhu Sheik Mujibur Rahman Agricultural University, Gazipur, Bangladesh

Abstract/ Executive Summary:

Botrytis gray mold disease like symptoms appeared on gladiolus during 2012 and 2013 crop season grown in Jessore regions of Bangladesh. The disease caused spots on leaves, flower buds and inflorescence. In severe infection, the disease caused both flower and leaf blight and corm rot. Botrytis gladiolorum was consistently isolated from infected gladiolus plants. For confirmation of the disease, Koch’s postulate was performed through artificial ino-culation of healthy leaves of gladiolus grown in pots in a glass house. Conidial suspension of B. gladiolorum isolated from naturally infected plants used as inocula for inoculation. Characteristic symptoms of Botrytis blight developed on inoculated gladiolus plants were identical as recorded from the field. Based on inoculation test it was confirmed that the disease was Botrytis blight of gladiolus and the causal fungus was B. gladiolorum. This is the first record on the occurrence of Botrytis blight and its causal pathogen, B. gladiolorum in Bangladesh.

Keywords: Botrytis gray, Botrytis gladiolorum, Incidence

Location: Plant Pathology Division, Bangladesh Agricultural Research Institute (BARI), RARS, Jessore

Start Date: 00-00-2012

End Date: 00-00-2013

Program Area: Pest Management

Commodity: Gladiolus

Objectives:

An investigation was conducted to identify the disease of Gladiolus (Gladiolus hortulanus) during the flower season of 2013 in Jessore region of Bangladesh.

Methodology:

Diseased samples of leaf, flower buds and stem of gladiolus were collected from farmers’ fields of Jessore regions. The fungus associated with the specimens was isolated following tissue planting methods on potato dextrose agar. Collected leaf specimens were cut into small pieces, sterilized with 1.0% chlorox (NaHCl) solution for 1 min, rinsed in sterile distilled water for three times and placed in Petri dishes containing PDA. The isolated fungus was purified following hyphal tip method. To identify the fungus, morphological characters such as conidiophore length, conidial and sclerotial dimensions were recorded, and the associated fungus was identified based on the morphology. Pathogenicity of the isolated fungus was performed under control conditions by inoculating healthy gladiolus with spore suspension of B. gladiolorum isolates. Gladiolus plants were grown in earthen pots (20 cm height and 20 cm rim diameter). The isolates were multiplied on PDA in Petri dishes. Ten days after incubation, conidia were harvested from the cultures by flooding the plates with sterilized distilled water and scraping with sterilized glass slides. The conidial suspension was filtered through muslin cloth to remove mycelium fragments. The suspension was adjusted to 6x104 conidia ml^-1using sterilized distilled water. At flowering stage, apparently healthy gladiolus leaves were inoculated with the conidial suspension. For inoculation, the inoculum suspension was sprayed over the plants. Plants under control were sprayed with plain water. Both inoculated and control plants were covered with polythene sheet to keep the plants humid for 48 hours. The pots with plants were placed in a glass house having ambient temperature of 20-22⁰C until development of symptoms. Characteristic symptoms of the disease appeared within 12 days of inoculation. The inoculated fungus was re-isolated from the inoculated plant parts showing characteristic symptoms following the procedures as mentioned earlier. Pieces of leaf specimens were also plated on moist blotting paper in Petri dishes and incubated at 21⁰C. The fungus grew on the leaf samples were isolated, purified and morphological characteristics of the fungus were recorded.

Source of Information: Bangladesh J. Plant Pathol. 29 (1&2):5-10, 2013; ISSN 1012-9279

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Findings of the present investigation clearly revealed that Botrytis blight caused by B. gladiolorum regularly attacks the gladiolus plants in Jessore regions of Bangladesh. However, the disease has not yet been reported from the country. So, the present report may be considered as the new record of Botrytis blight of gladiolus and its causal fungus, B. gladiolorum in Bangladesh.

Knowledge Management: Journal