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Occurrence of Leaf and Pod Diseases of Dalbergia Sissoo in Bangladesh

Shamim Shamsi
Department of Botany, University of Dhaka-1000, Bangladesh.

Razia Sultana
Department of Botany, University of Dhaka-1000, Bangladesh.

Rumana Azad
Department of Botany, University of Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

A study was conducted to identify fungi associated with diseased leaves and pods of sisso tree (Dalbergia. sissoo Roxb (Sisam). Associated fungi were isolated and identified following standard methods. The associated fungi with leaves were Alternaria al-ternata (Fries) Keissler, Pseudocercospora dalbergiae (Sun) Yen, Chalara sp., Colletotrichum gloeosporioides (Penz.) Sacc., Cylindrocladium sp., Fusarium solani (Mort.) Sacc., Gebberella sp., Lasiodiplodia theobromae (Pat.) Griffon and Maubol, Mem-noniella sp., Phyllactinia dalbergiae Piroz. and its anamorph Ovulariopsis sissoo Shamsi, Sultana and Azad sp. nov, Tetra-ploa sp. and a rust fungus. From diseased pods, Colletotrichum gloeosporioides was isolated. This is the first report of associa-tion of Chalara sp., Memnoniella sp. and Tetraploa sp. with Sissoo from Bangladesh.

Keywords: Mycoflora, Diseases, Dalbergia sissoo

Location: Department of Botany, University of Dhaka

Start Date: 00-10-2008

End Date: 00-01-2010

Program Area: Pest Management

Commodity: Sisso

Objectives:

The present study was conducted to identify fungi associated with diseased leaves and pods of Sisso trees.

Methodology:

Diseased leaf and pod samples of Sisso were collected from Dhaka, Chittagong and Pabna districts during October 2008 to January 2010. Associated symptoms were recorded. The severity of disease on leaves was estimated visually using a 0-9 subjective scale, where 0= no infection, 1= up to 10% leaf area infected, 2= 10 – 20% leaf area infected, 3=20 – 30% leaf area infected, 4= 30 – 40% leaf area infected, 5= 40 – 50% leaf area infected, 6= 50 – 60% leaf area infected, 7= 60 – 70% leaf area infected 8= 70 – 80% leaf area infected and 9= 80% and above leaf area infected (Ghos et al. 2009). Fungi associated with diseased samples were isolated following “tissue planting method" and "blotter method”. In case of “tissue planting meth-od” fifty inocula each measuring 2 mm2 was cut from a particular specimen. The inocula were washed in sterile water and surface sterilized by dipping in 10.0% Clorox for 3-5 minutes. Three inocula were placed in each Petri plate containing sterilized potato dextrose agar (PDA) medium and incubated for 5-7 days at 25±2C. In “blotter method” moist chambers were made by placing two layers of filter paper on the bottom of the Petri plates. In each Petri plate, 5 surface sterilized inocula were placed and 10 plates were used. The inoculated plates were incu-bated at room temperature. The fungi growing out of the inocula were transferred to separate PDA plates and slants, and stored in a refrigerator for further studies. Prevalence of fungi associated with the specimens was expressed in percentage based on total number of leaf and pod samples checked. Identification of the isolated fungi was done using standard literature. All the specimens were preserved in the Herbarium, Mycology and Plant Pathology Section, Department of Botany, University of Dhaka, Bangladesh.

Source of Information: Bangladesh J. Plant Pathol. 28(1&2):33-40, 2012; ISSN 1012-9279

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Results of the present study revealed that at least 13 species of fungi are associated with diseased leves of Sisso tree. The leaf spot, anthracnose and powdery mildew are the major diseases of D. sissoo in Bangladesh. The environment of Savar is favorable for growth and development of most the fungi than Chittagong, Dhaka and Pabna. Association of Chalara sp., Memnoniella sp. and Tetraploa sp. with D. sis-soo is new records in Bangladesh.

Knowledge Management: Journal