Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Induced Breeding of Magur (clarias Batrachus)

UMMEY HAYAT SULTANA
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

An experiment on induced breeding of magur, Clarias batrachus was carried out at Rupal Integrated Aquafarm, Satrasia, Muktagacha, Mymensingh during the period from March to May, 2013 by using PG and ovaprim. Twenty pairs of brood fish of, C. batrachus were used in this experiment. Brood fish were reared up to maturation for spawning operation in the farm by providing artificial diet (produced in the farm containing 30% protein) for 4-5 months before on set of breeding season. In the present experiment, the brood fish were induced with 1mg Ovaprim/Kg for male and 2 mg Ovaprim/Kg for female in T1; 30 mg PG/Kg for male and 80 mg PG/Kg for female in T2; 1mg Ovaprim/Kg for male and 80 mg PG/Kg for female in T3 and 30 mg PG/Kg for male and 2 mg Ovaprim/kg for female in T4. The brood fishes were injected with single injection in all treatments. When brood fishes were injected with Ovaprim and PG resulting less fertilization rate (79%) in T3. When the brood fish were injected with PG resulting highest fertilization rate (95%) in T2. For induced breeding of C. batrachus a dose of 30 mg PG/Kg male and 80 mg PG/Kg female fish in T2 was found to be most effective among the four treatments. Fertilization rates were 80%, 95%, 79% and 88% and hatching rates were 45%, 73%, 65% and 49.4% in treatments T1, T2, T3 and T4, respectively. The incubation period for fertilized eggs were 23-24 hrs in all the treatments. Higher fertilization rate (95%) and hatching rate (73%) were also found in case of PG-PG combinations in T2.

Keywords: INDUCED BREEDING,Clarias batrachus, Bangladesh

Location: Rupal Integrated Aquafarm, Satrasia, Muktagacha, Mymensingh.

Start Date: 01-03-2013

End Date: 30-05-2013

Program Area: Animal Health and Management

Commodity: Cat fish

Objectives:

To breed C. batrachus by using inducing agents

Methodology:

The experiment was carried out at the Rupal Integrated Aquafarm, Satrasia, Muktagacha, Mymensingh during the period from March to May 2013. In this experiment two type of tanks were used. One type for conditioning of the brood fishes and another type were used for spawning purpose. Both conditioning and spawning tanks were with continuous flow of water. Incubation trays were used to study fertilization rate. For earning of newly hatched spawn four trays (28 cm × 43 cm × 10 cm) were used and the mortality and deformities were observed and counted. Experiments fish: Experimental fish is indigenous magur Clarias batrachus. Duration of the experiment: This experiment was done from 01.03.2013 to 30.05.2013 in Rupal Integrated Aquafarm. Brood fish maintenance: Broods were collected from different ponds of this hatchery which was cultured last two or three years and reared in the earthen ponds at the rate of 200-250 fish/decimal. The fish was supplied with balanced nutritious feed (produced in the farm containing 30% protein) at the rate of 3-5% body weight/day until maturity during January-May, 2013. Brood fish collection: Brood fishes were collected from the ponds of the farm in the late morning and were kept in plastic bucket with water. Then the fishes were selected carefully for further use. Only conspicuous, healthy uninjured, matured fishes were selected for induced breeding. The fishes containing spot on their body and unhealthy were discarded from being selected. Inducing agent: The fish were carefully handled to avoid any injury and secondary infection. The plastic bucket was covered by a clean cloth to protect the fish from being escaping and become injured. For induced breeding of the fish Clarias batrachus two main inducing agents were used: 1. PG (Pituitary gland) 2. Ovaprim Cleaning of the tank: Prior to shifting the brood male and female in the tank for conditioning for spawning purpose, the tank were washed properly for strictly maintained hygienic and germ free condition. The following procedure was used to clean the tanks: At first the tanks were washed properly with clean water. Then half of the tanks were filled with water and kept for 300 minutes. Then the water was splashed to the wall of the tanks and cleared with water. After that, lime and potassium permanganate was spread in the tanks and the walls were rubbed with brush. Then the tanks were left for 15 minutes in the condition. After that the tanks were rewashed two to three times with water. The tanks were again washed with salt water. After washing again with clean water the tanks were finally filled with clean water. Nets, same size of the tanks were placed in the tanks to remove fish easily. Conditioning of brood fish: The fishes were transferred in the tanks for conditioning for 4-5 hours. During conditioning the male and female fishes were kept separately, prior to administration of the inducing agents. Flash of water in the tank was given continuously during conditioning period. No feed was supplied to the brood fishes during conditioning. Induced breeding: The induced breeding experiment was conducted from 13.05.2013 to 20.05.2013 by using PG and Ovaprim at different doses and combinations. For each treatment a group consisting of 6 male and 5 female were used. Preparation of inducing agent and mode of administration: Processed dried carp PG and Ovaprim were collected from market (Reeaj Enterprize, Boro Mosjid Road, Muktagacha and Durjoy Fisheries and Veterinary Medicine Corner, Doyel shopping complex limited, station road, Mymensingh) and was kept in a refrigerator to be used for breeding purpose as inducing agent. The required amounts to be weighed out were calculated. The required amount of PG was homogenized in a tissue homogenizer with a small amount of distilled water. The suspension of homogenized gland were poured in a centrifuge tube and diluted with distilled water to obtain the desired concentration and were centrifuged for 3 minutes at a rate of 1000 rpm. The supernatant was poured in a vial made of glass. The supernatant solution was then injected to the brood fishes by taking out in 1 ml syringe. The dose was 30 mg PG/kg for male and 80 mg PG/kg for female and 2 mg Ovaprim/kg for female and 1 mg Ovaprim/kg for male were used for injection. Hormone injection: For injection, the broods were taken out from the conditioning tank by a sterile scoop net. Male and female from separate tanks were kept in two different buckets before injecting the fish. The fishes were then weighed individually (Male and female separately) for each experiment. The injection with PG and ovaprium solution was administered intra-muscularly. During injection the brood fishes were handled smoothly. The fishes were kept in foam and the head region of the fish was wrapped by a wet and soft towel. The administration of injection was done carefully, with an 1ml syringe. In case of injection, needle was inserted at about 45° angle. The injection was pushed on the caudal region and ventral region. Treatments: Four separate treatments were used with different combination of hormone dose. The different doses were Ovaprim-Ovaprim, PG-PG, Ovaprim-PG and PG-Ovaprim. Treatments-1: The treatment was conducted at 5.30 pm on 13.05.13. In this treatment the combination of hormone used was Ovaprim-Ovaprim. The doses used for male fishes were 1 mg/Kg and for female fishes was 2 mg/Kg. Five female and six males were injected and after injection they were transferred into spawning tank. Treatment-2: The treatment was conducted at 5.30 pm on 16.05.13. In this treatment the combination of hormone used was PG-PG. The doses used were 30 mg/Kg for male and 80 mg/Kg for female. Treatment-3: The treatment was conducted 5.45 pm on 17.05.13. In this treatment the combination of hormone used was Ovaprim-PG. The doses used were 1 mg/Kg for male and 80 mg/Kg for female. Treatment-4: The treatment was conducted at 6.00 pm on 20.05.13. In this treatment the combination of hormone used was PG-Ovaprim. The doses used were 30 mg/Kg for male and 2 mg/Kg for female. Reproductive behavior: After injection the male and female fishes were transferred in the spawning tank together. The fishes show some breeding behavior in the spawning tank like moving, cashing, bending etc. The reproductive behavior and activities of the male and female fishes C. batrachus were studied by continuous observation. Spawning and Fertilization: In this experiment fish were allowed to spawn naturally. Both the male and female were kept in the same spawning tank and fishes were allowed to release eggs and milt, fertilize naturally in the tank. But, they didn’t response to fertilize naturally, the females were kept cut from the tank for stripping. The mature eggs were stript out on a bowl. The testes from males were dissected out and cutting to piches and mixed with matured eggs for fertilization. Release of spent fishes: When the spawning was completed female breeders were treated with 0.1 g/L solution of KMnO₄ for 1-2 minutes and after that the brood fishes were shifted to the pond from where they were caught for breeding purposes. Rate of fertilization: The rate of fertilization were calculated by direct counting method taking sub sample. The eggs were spread on a tray was apparently divided into 16 equal portion. Any 4 equals were selected randomly and then eggs were counted from this portion. Finally the average number of eggs was multiplied by 16 and total number of eggs was calculated. After one hour of fertilization the transparent eggs were considered as fertilized eggs whereas, the opaque eggs were considered as dead eggs. From this the rate of fertilization was calculated. Incubation and hatching: The eggs were collected for incubation and hatching. The eggs were spread on the floor of the tank by using a feather. The water temperature was recorded (30°C) by using a Celsius thermometer. Eggs were kept under shower of water by piercing a slender pipe and water run through it. After 22-24 hours of incubation the eggs were hatched and the hatchling came out from the eggs. The hatchling was counted by direct counting method. At first the hatchling was taken in a white enamel bowl by siphoning method. From the counting of hatchling the rate of hatching was calculated.

Source of Information: MS Thesis, Roll No. 11 Fish Aqua JJ 03 M, Registration No.: 31718 Session: 2004-05, Department of Aquaculture, Bangladesh Agricultural University, Mymensingh

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Results of present study indicated that C. batrachus could be easily induced to spawn by the technique followed here: 30 mg PG/kg body weight and 80 mg PG/kg body weight for both male and female fish.

Knowledge Management: Thesis