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Exogenous Salicylic Acid Alleviates Salt Stress-induced Oxidative Damage in Brassica Napus by Enhancing the Antioxidant Defense and Glyoxalase Systems

Mirza Hasanuzzaman
Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan; Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh

Md. Mahabub Alam
Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan;

Kamrun Nahar
Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan; Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh

Jubayer-Al-Mahmud
Department of Agroforestry and Environmental Science, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh

Kamal Uddin Ahamed
Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207, Bangladesh

Masayuki Fujita
Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan

Abstract/ Executive Summary:

Regulatory roles of exogenous salicylic acid (SA) on the antioxidant defense and methylglyoxal (MG) detoxification systems were investigated in rapeseed seedlings (Brassica napus L. cv. BINA Sharisha 3) grown under salinity. Ten-day-old seedlings, grown in petri dishes, were supplemented with SA and salt (100 and 200 mM NaCl) separately and in combination for 48 h. After treatment, MDA and H₂O₂ content, non-enzymatic and enzymatic components of antioxidant and glyoxalase enzymes were measured. The ascorbate (AsA) content of the seedlings was decreased significantly with increased salt stress. Salt stress resulted marked raise in the levels of H₂O₂ and lipid peroxidation (MDA). The amount of glutathione (GSH) and glutathione disulfide (GSSG) were increased with an increase in the level of salt stress, while the GSH/GSSG ratio was decreased. Imposition of salt stress causes decrease in most of the antioxidant enzymes except for the ascorbate peroxidase (APX) and glutathione S-transferase (GST). However, compared to salt stressed seedlings alone, exogenous SA treatment in combination with salt stress enhanced AsA and GSH contents; GSH/GSSG ratio; and activities of antioxidant enzymes such as monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST), glutathione peroxidase (GPX), catalase (CAT), glyoxalase I (Gly I), and glyoxalase II (Gly II). This study indicates that exogenous application of SA is an effective protectant in improving the activities of both antioxidant defense and glyoxalase enzymes in coffering salt stress tolerance in B. napus.

Keywords: Abiotic stress; Antioxidants defense; Methylglyoxal; Oxidative stress; Phytohormones, Reactive oxygen species

Location: Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Mustard

Objectives:

To study the effects of exogenous SA on the antioxidant defense and glyoxalase systems in rapeseed (B. napus) seedlings grown under saline media.

Methodology:

Plant materials and stress treatments - Rapeseed (Brassica napus L. cv. BINA Sharisha 3) seeds of uniform size were selected and surface-sterilized with 70% ethanol for 10 min followed by washing several times with sterilized distilled water. The seeds were then sown in petri plates (9 cm) lined with 6 layers of filter paper moistened with 10 mL of distilled water for germination for two days. Germinated seedlings were then allowed to grow under controlled condition (light, 100 μmol photon m–2 s–1; temperature, 25±2°C; RH, 65–70%) that contained 10,000-fold diluted Hyponex solution (Hyponex, Japan). After 10 days, two sets of seedlings were subjected to two different levels of salt stress viz. 100 and 200 mM NaCl in Hyponex solution. First set of seedlings were grown without SA. Another set of seedlings were sprayed with 100 μM SA (Wako, Japan) containing 0.02% Tween 20 (Polyoxyethylenesorbitan monolaurate, Wako, Japan). Each set of seedlings was sprinkled twice a day. Control plants were grown with in Hyponex solution only. Data were taken after 48 hours of treatment. The experiment was repeated three times under the same conditions.

Source of Information: AJCS 8(4):631-639 (2014); ISSN:1835-2707

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Based on the results, it is concluded that the antioxidant system and glyoxalase cycle are co-regulated to control the ROS and MG levels under salt stress condition as influenced by SA. In spite of being a well-known hormone and signal molecule, which may provide protection under biotic or abiotic stress condition still there are many gaps in grasping the basic mechanisms through which SA confer abiotic stress tolerance and its overall effects in plants should be explored. Exact mechanism of the mode of action of SA is still poorly understood, especially because it may differ in the different species, and may also depend on the environmental factors. Therefore, complete elucidation of the role of SA as well as detailed protective mechanisms would be helpful for developing stress tolerance in plants. The appropriate dose and method of SA application in plants are also matter of elucidation.

Knowledge Management: Journal