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Histology of the Spleen of Indigenous Dog (canis Familiaris) of Bangladesh

S. K. Das
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2002, Bangladesh.

M. S. Alam
MS Student
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. N. Islam
Department of Anatomy and Histology, Sylhet Government Veterinary College, Sylhet, Bangladesh.

M. A. Awal
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2002, Bangladesh.

Abstract/ Executive Summary:

The present research was conducted in the Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh from July to December 2003 with a view to study the detail histology of the spleen of indigenous dog of Bangladesh. Tissues were collected from the different parts of the spleen from the dogs killed after proper euthanasia. The adult experimental dogs were apparently healthy and disease free. The tissues were passed through different stains (Van Gieson's and Verhoeffs, Bielschowsky's Silver and Potassium Ferrocyanide stains) for detail histological study. The present study revealed that the spleen was encircled by capsule and trabeculae with the internal structures of white pulp, red pulp and sinusoids that are nearly similar as found in cattle. The capsule was composed of collagen, elastic, reticular and smooth muscle fibers. The trabeculae originated from the hilus that usually contained major vessels but was poor in smooth muscle fibers. The white pulp had a fine meshwork of reticular connective tissue containing mainly lymphocytes of various sizes. Meshworks of fine reticular fibers were observed throughout the red pulp in the present study. Sinusoids of the spleen of indigenous dog were of different shapes and sizes and widely distributed within the red pulp and had both arterial and venous communications.

Keywords: Spleen, Histology, Dog

Location: Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh.

Start Date: 00-07-2003

End Date: 00-12-2003

Program Area: Variety and Species

Commodity: Animal

Objectives:

To study the histology of the spleen of indigenous dog of Bangladesh.

Methodology:

The present study was performed in the Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh from July to December 2003 on the spleen of 16 adult and healthy indigenous dogs (Canis familiarity of Bangladesh. Five pieces of tissues (about 1 cm³ in size) representing different areas of spleen from each dog were collected immediately after the dogs were killed by euthanasia and fixed in the Buin's fluid. The tissues were then dehydrated in graded alcohol, cleared in xylene, embedded in paraffin and finally the sections were cut at 6 micron thickness using rotary microtome (Model 820, USA). The sections were then stained with Van Giesen's stain for collagen fibers, Verhoeff's stain for elastic fibers, Bielschowsky's silver method (Foot's modification) for reticular fibers and Modification of Mallory's Reaction or Potassium Ferrocyanide stain for iron pigments. Detail histological study was performed using high and power light microscopy (X10 to X400).

Source of Information: Bangl. J. Vet. Med. (2005), 3 (1): 59-61.

Article Link (Online Journal): http://dx.doi.org/10.3329/bjvm.v3i1.11356

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present study revealed that the spleen was encircled by capsule and trabeculae with the internal structures of white pulp, red pulp and sinusoids that are nearly similar as found in cattle. The capsule was composed of collagen, elastic, reticular and smooth muscle fibers. The trabeculae originated from the hilus that usually contained major vessels but was poor in smooth muscle fibers. The white pulp had a fine meshwork of reticular connective tissue containing mainly lymphocytes of various sizes. Meshworks of fine reticular fibers were observed throughout the red pulp in the present study. Sinusoids of the spleen of indigenous dog were of different shapes and sizes and widely distributed within the red pulp and had both arterial and venous communications.

Knowledge Management: Journal