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Effects of Different Edible Oils on Growth Performance, Different Organ Weight and Serum Transaminases in Rats

S. K. Saha
Kazi Farms Bangladesh Ltd.

N. Ahmad
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

S. Majumder
Department of Agricultural Statistics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. Z. Hosain
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. A. Miah
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

The effects of different edible oils on body weight gain, different organ weight and serum transaminases were studied on 25, one- month old Long Evans male rats during the period from 3 April to 28 May 2003. The rats were randomly assigned to one of five equal groups (n = 5) as A, B, C, D and E of which group A was considered as control and fed with rat pellets (ICDDR, B) and others were supplemented with oils as soybean (group B), palm (group C), coconut (group D) and mustard (group E) at a concentration of 7.5% with pellets for 8 weeks. The results revealed significant (p <0.(01) increase in weight gain of the non supplemented rats (control). The mean heart weight of the non supplemented rats (control) was significantly (p <0.05) higher than those of the treated groups. The mean liver weight of non supplemented, coconut and mustard oils treated rats were significantly (p <0.05) higher than those of soybean and palm oil treated groups. The mean kidney weight of the mustard oil treated rats was significantly (p <0.05) lower than those of other treated and control groups. No significant differences (p <0.05) were observed among the groups in respect to SGOT and SGPT activities.

Keywords: Edible oil, Rat, Body weight, Organ weight, Serum transaminases

Location: Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh.

Start Date: 03-04-2003

End Date: 28-05-2003

Program Area: Animal Health and Management

Commodity: Rats

Objectives:

To study the effects of different edible oils on growth performance in rats.

Methodology:

A total of 25, one month aged Long Evans male rats purchased from ICDDR, B was used in this experiment for the period from 3 April to 28 May 2003. The rats were randomly assigned to one of five equal groups (n = 5) and numbered them as A, B, C, D and E. Group A was considered as control and fed with rat pellet. Others were treated with soybean oil (group B), palm oil (group C), coconut oil (group D) and mustard oil (group E) at a concentration of 7.5% with rat pellet for 8 weeks. Body weight of each rat was taken before treatment and then weekly until the last day of experiment using an electrical balance. At the end of experimental period the rats were starved overnight. A series of sterile test tubes were taken for collection of blood. Sequential anaesthesia was done with 3-4% halothane using self adopted technique and the blood was collected by incising the abdominal aorta after opening the abdominal cavity. The selected organs (heart, liver and kidney) collected by tissue forceps and weighed after removing the unwanted tissues. The blood containing tubes were placed in slanting position at room temperature for 6 hours. The tubes were then incubated overnight in the refrigerator (4^oC). The sera samples were separated and centrifuged to get rid of unwanted blood cells where necessary. Sera were kept at -20^oC until analysis for enzymes. Analysis of enzymes (Serum Glutamate Oxaloacetate Transaminase - SGOT and Serum Glutamate Pyruvate Transaminase - SGPT) was performed in a quickest possible time using Humalyzer 2000 (Human type, Germany). A randomized complete block design (RCBD) with multiple observations per cell was applied. An analysis of variance (ANOVA) was done for testing the significance of the difference among the mean body weight gains of the experimental rats under the different treatments. A comparison of the organ weights in the different treatment groups of the experimental rats was made by performing Duncan's Multiple Range Test (DMRT). Enzyme systems in the different treatment groups were also compared by DMRT.

Source of Information: BangI. J. Vet. Med. (2005), 3 (1): 79-81.

Article Link (Online Journal): http://dx.doi.org/10.3329/bjvm.v3i1.11391

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results revealed significant (p <0.(01) increase in weight gain of the non supplemented rats (control). The mean heart weight of the non supplemented rats (control) was significantly (p <0.05) higher than those of the treated groups. The mean liver weight of non supplemented, coconut and mustard oils treated rats were significantly (p <0.05) higher than those of soybean and palm oil treated groups. The mean kidney weight of the mustard oil treated rats was significantly (p <0.05) lower than those of other treated and control groups. The present findings indicated that edible oils had no effect in increasing body weight and changes in transaminase activity but had little effect on organ weight in rats.

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