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Effect of Carrier Materials and Temperature on Shelf Life of Phosphate Solubilizing Bacterial Inoculants.

Dr. Md. Azizul Haque
Principal Scientific Officer
Soil Science Division, Bangladesh Institute of Nuclear Agriculture (BINA), BAU campus, Mymensingh.

Dr. M. A. Sattar
Ex-DG,
BINA, Mymensingh

M.R.Islam
Professor
Dept. of Soil Science, BAU, Mymensingh

Abstract/ Executive Summary:

A study was undertaken to select a suitable carrier materials for survival of different phosphate solubilizing bacterial (PSB) inoculants at two storage temperature for long duration.The experiment was conducted during September 2009 to August 2010 at the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. Three PSB inoculants (MR1, MW1 and mixture of MR1 and MW1) were tested against six carrier materials (CM₁: Peat soil, CM₂: Pond slurry, CM₃: Pressmud, CM₄: 50% CM1 + 50% CM2, CM₅: 50% CM1 + 50% CM3 and CM₆: 50% CM2 + 50% CM3) at room temperature (10~37 ⁰C) and air condition (15 ⁰C). The experiment was carried out in a completely randomized design with three replications for each treatment. The viable cells of PSB in carrier materials were counted at 15, 30, 60, 180, 270 and 360 days after incubation. Maximum viable cells were recorded into the carrier material CM1 (peat soil) with the mixed PSB (MR1+MW1) cultures (28.8 × 10¹⁰cfu g^-1 carrier) at 30 days after incubation (DAI) at room temperature followed by the culture MR1 (28.3 × 10¹⁰cfu g^-1 carrier) in the same DAI at same condition. All the PSB inoculants can be stored with desired (≥10⁶cfu g^-1) population in all the tested carrier materials up to 270 days (9 months) at room temperature and 360 days (one year) at air condition. The results indicated that all the PSB cultures showed longer shelf life with desired (≥10⁶cfu g^-1) population at air condition than the room temperature with six carrier materials. Among the carrier materials, peat soil alone showed the best performance as carrier materials for survival of all the PSB inoculants followed by either its mixture with pond slurry or pressmud at the ratio of 1:1. Hence, mixture of peat soil either with pond slurry or pressmud can be used at the ratio of 1:1 as alternate carrier material for PSB inoculants than peat soil alone.

Keywords: PSB, Carrier materials, Survival, Temperature

Location: Soil Microbiology Lab., Soil Science Division, BINA, Mymensingh.

Start Date: 00-09-2009

End Date: 00-08-2010

Program Area: Crop-Soil-Water Management

Commodity: Bio fertilizer

Objectives:

To select a suitable carrier materials for survival of different phosphate solubilizing bacterial (PSB) inoculants at two storage temperature for long duration.

Methodology:

Three PSB inoculants (MR1, MW1 and mixture of MR1 and MW1) were tested against six carrier materials (CM₁: Peat soil, CM₂: Pond slurry, CM₃: Pressmud, CM₄: 50% CM1 + 50% CM2, CM₅: 50% CM1 + 50% CM3 and CM₆: 50% CM2 + 50% CM3) at room temperature (10~37 ⁰C) and air condition (15 ⁰C). The experiments were carried out in a completely randomized design with three replications for each treatment. The viable cells of PSB in carrier materials were counted at 15, 30, 60, 180, 270 and 360 days after incubation. Pond slurry and pressmud were separately ground into fine powder after drying and they were passed through 100 mesh sieve. Carrier materials were neutralized with lime and pH was adjusted at 7.2. Then all the carrier materials were sterilized with autoclave at 121⁰C at 15 PSI for 3 hrs alternately for three days. About 75 g of each sterilized carrier material was taken into polyethylene bag as per treatment plan. All the bags were sealed with the electrical sealing machine aseptically. 25 ml of each PSB broth culture was aseptically dispensed into the previously packed carrier materials with an auto dispenser as per treatment plan. In case of mixed inoculants, two single PSB broth cultures (MR1 and MW1) were mixed at the ratio of 1:1. Then it was dispensed @ 25 ml into the carrier materials. All the inoculated bags were rubbed by hand to mix the broth with the carriers properly. Uninoculated bags were also run for the control. Inoculated or uninoculated (control) bags were divided into two groups, one was incubated at room temperature and another was incubated in air condition. The carrier materials were sampled aseptically at 15, 30, 60, 180, 270 and 360 days after incubation from both the groups. Viable cells of PSB were counted on Pikovskaya’s solid medium containing tricalcium phosphate (TCP) through serial dilution techniques (Pikovskaya, 1948). The colonies showing solubilization zone (halo zone) were counted as viable phosphate solubilizers. The survival of PSB cells were expressed in cfu g^-1 oven dry carrier (colony forming unit). The numbers of cells per gram carrier materials were transferred into logarithmic value then data were analyzed individually followed by one way ANOVA.

Source of Information: Bangladesh J. Nuclear Agric. 25&26:77-84.(2012)

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 50,000/-TK

Total Budget (Tk.) : 50,000/-

Achievement/Findings:

The results indicated that all the PSB cultures showed longer shelf life with desired (≥10⁶cfu g^-1) population at air condition than the room temperature with six carrier materials. Among the carrier materials, peat soil alone showed the best performance as carrier materials for survival of all the PSB inoculants followed by either its mixture with pond slurry or pressmud at the ratio of 1:1. Hence, mixture of peat soil either with pond slurry or pressmud can be used at the ratio of 1:1 as alternate carrier material for PSB inoculants than peat soil alone.

Knowledge Management: Journal