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Effect of Certain Haematinics on Body Weight and Haemato-biochemical Changes in Laboratory Mice

M. Jahan
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

N. Ahmad
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. Myenuddin
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

A total of 24, six weeks old mice were used to study the body weight and haemato-biochemical changes following administering of different haematinics (CuSO₄, FeSO₄ and Vitamin B₁₂). The experiment was performed in Physiology laboratory, Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, from February to March 2006. They were randomly assigned to one of four equal groups (n = 6). In addition to normal rat pellets Group A was supplemented with CuSO₄ @ 0.6mg/mice/day orally, Group B with FeSO₄ @ 1.2mg/mice/day orally, and Group C with Vitamin B₁₂ (Cytamin^®, Glaxo) @ 0.004mg/mice intramuscularly at every 7 days. Group D was considered as control and was also supplemented with rat pellets. Increased body weight was observed in all haematinic treated mice but group B had a significant (p <0.05) higher weight gain compared to the control. TEC and PCV increased significantly (p <0.05) in all the treatment groups compared to the control. Statistically insignificant (p >0.05) increase in TLC was also recorded in all the treated groups. Hb concentration increased for all the treatment groups. The ESR values of the experimental mice were negligible in first hour. The serum transaminases (SGPT and SGOT) increased in all treated groups but the difference in group A was significant (p < .05) compared to the control. Blood urea was recorded significantly (P < .01) higher for group A and lower for group C compared to the control. This study can be helpful to study the effect of particular haematinic on animal especially on simple stomach animal and human being as mice is used as human and animal model.

Keywords: Haematinics, Haemato-biochemical parameters, Mice

Location: Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh.

Start Date: 00-02-2006

End Date: 00-03-2006

Program Area: Animal Health and Management

Commodity: Rats

Objectives:

To study the effect of different haematinics (copper sulphate, ferrous sulphate, vitamin B₁₂) on body weight, haematological parameters and biochemical parameters in mice.

Methodology:

A total old twenty four (24), six weeks old mice were randomly assigned to one of four equal groups (n = 6). All the groups were fed with rat pellet and additional haematinics were supplied to group A with CuSO₄ @ 0.6mg/mice/day orally, B with FeSO₄ @ 1.2mg/mice/day orally and C with vitamin B₁₂ (Cytamin^®, Glaxo) @ 0.004mg/mice intramuscularly at every 7 days for 30 days from February to March 2006 in the laboratory of the Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. Group D was considered as control. Pure drinking water was also made available. The body weight of each mouse was measured with the help of electric balance on 7 days interval during the 30 days experimental period. The body weight was taken before feeding in the morning and expressed in gram (g). Blood samples from 3 mice of each group were collected at the end of experimental period. The mice were starvated overnight. A series of sterile test tubes containing anticoagulant (Double oxalate salt) at a ratio of 1:10 was taken. General anesthesia of mice was done using ether. The blood was collected directly from heart after opening the abdominal and thoracic cavity. The blood was kept in ice bath till examination. Haematological studies such as total erythrocyte count (TEC), total leukocyte count (TLC), estimation of hemoglobin (Hb) by acid hematin method and erythrocyte sedimentation rate (ESR) were performed within two hours of blood collection as described by Lamberg and Rothstein (1977). Rest 3 mice from each group were used for biochemical studies. Blood was collected from tail vein for SGOT, SGPT and urea determination. The samples were analyzed using Reflotron^® autoanalyzer (Roche Diagnostics GmbH, D-68298 Mannheim, Germany). The mean values of the body weight, haematological and biochemical parameters in the different treatment groups were compared with the control by conducting Student’s t -test.

Source of Information: Bangl. J. Vet. Med. (2007), 5 (1 & 2): 103–105

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Achievement/Findings:

Increased body weight was observed in all haematinic treated mice but group B had a significant (p <0.05) higher weight gain compared to the control. TEC and PCV increased significantly (p <0.05) in all the treatment groups compared to the control. Statistically insignificant (p >0.05) increase in TLC was also recorded in all the treated groups. Hb concentration increased for all the treatment groups. The ESR values of the experimental mice were negligible in first hour. The serum transaminases (SGPT and SGOT) increased in all treated groups but the difference in group A was significant (p < .05) compared to the control. Blood urea was recorded significantly (P < .01) higher for group A and lower for group C compared to the control. This study can be helpful to study the effect of particular haematinic on animal especially on simple stomach animal and human being as mice is used as human and animal model.

Knowledge Management: Journal