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Histomorphology and Histochemistry of Testis of Indigenous Bull (bos Indicus) of Bangladesh

M. R . Gofur
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. Z. I. Khan
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. R. Karim
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. N. Islam
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2002, Bangladesh.

Abstract/ Executive Summary:

Histomorphological and histochemical features of testes were studied in six adult indigenous bulls (Bos indicus) of two different age groups, 1 year 9 months to 2 years of age (group A) and 2 years 3 months to 2 years 6 months of age (group B) during the period from September 2006 to April 2007 by using Hematoxylin and Eosin (H&E) stain, Verhoeff’s stain, Van Gieson’s stain and Periodic Acid-Schiff Reaction (PAS) stain. The testes were surrounded by visceral layer of tunica vaginalis (consisted of mesothelium and connective tissue) and tunica albugenia mainly composed of collagen fibers. The seminiferous tubules were tortuous, two ended loops and varying in appearance and the wall of tubules consisted of lamina propria, basemen tmembrane supported by reticular fibers and a lining of complex stratified epithelium consisted of sertoli cells and spermatogenic cells. The sertoli cells are irregulary columnar cells, extended from basal lamina to lumen of tubules and the spermatogenic cells situated between the sertoli cells in an orderly manner with four to eight layers occupying the space between the basal lamina and the lumen of the tubules. There was presence of both spermatid and spermatozoa in the lumen of some seminiferous tubules of testes of bulls of both age groups. The spermatogonia, primary spermatocytes and secondary spermatocytes showed more staining affinity than the spermatid in routine staining technique. The basement membrane of tubules, spermatid and spermatozoa showed positive affinity whereas spermatogonia, primary spermatocytes and secondary spermatocytes showed negative affinity to PAS stain. The interstitial tissues located between the sminiferous tubules, consisted of connective tissue network, mainly composed of collagenous and reticular fibers; blood and lymph vessels with Leydig cells. The Leydig cells were present as single or groups within inter tubular spaces. It was concluded that the thickness of tunica albuginea, the stratification of growing spermatogenic cells and cross sectional length and breadth of the seminiferous tubules of testes were higher in the bull of group B than group A and the number of Leydig cells were more in the testis of group A than group B and in between left and right testes, the thickness of tunica albuginea and cross sectional length and breadth of the seminiferous tubules were higher in the left testis but the number of Leydig cells was higher in right testis in both age groups.

Keywords: Testis, Seminiferous tubule, Leydig cell, Indigenous bull

Location: Laboratory of the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh.

Start Date: 00-09-2006

End Date: 00-04-2007

Program Area: Animal Health and Management

Commodity: Bull

Objectives:

To understand the histology and histochemistry of testis of indigenous bull which provides valuable information to the anatomist, pathologist and theriogenologist.

Methodology:

The present research was carried out on testes (both left and right) of six indigenous bulls (Bos indicus) of two different age after onset of the puberty for histological and histochemical studies during the period from September 2006 to April 2007 at the Laboratory of the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. Six adult indigenous bulls were selected in different local slaughter houses at Mymensingh district in Bangladesh for histomorphological and histochemical study of testes. The bulls were then divided into two groups (group A and group B) on the basis of their age. Group A included the bulls (n = 3) of 1 year 9 months to 2 years of age (bearing 2 permanent incisor teeth) and group B included the bulls (n = 3) of 2 years 3 months to 2 years 6 months of age (bearing 4 permanent incisor teeth). The age was determined by dentition according to eruption chart. Immediately after slaughter, the testes were collected. Then the testes were cut into small pieces. Small pieces of testicular tissue which were free from pathological lesion were used in this study. The small pieces of testes were fixed in the “Bouin’s fluid”. After fixation, the selected samples were processed in the laboratory following standard histological method, and the paraffin sections were then cut at 4 µm thickness using sliding microtome (MIC 509, Euro me x, Japan). After cutting, the sections were floated on luke-warm water in a floatation bath at 37°C for stretching, then the sections were attached on cleaned glass slides using egg albumin and dried on a hot plate of slide warmer boxes. The sections were then stained with routine Hematoxylin and Eosin stain for histomorphological study and with special stains such as Van Gieson’s stain, Verhoeff’s Elastic stain and Periodic Acid-Schiff Reaction (PAS) stain for histochemical study of the testes of indigenous bull. After staining, the sections were dehydrated in ascending grades of alcohol, cleared in xylene and mounted with “DPX”. The stained sections of testes were studied thoroughly under light microscope using 10 and 40 objectives. The thickness of tunica albuginea and histological sectional length and breadth of the seminiferous tubules were measured using calibrated scale by adjusting ocular grid and stage microscope. The Leydig cells in the sections of both left and right testes of both age groups were counted in 20 fields using ocular micrometer at a magnification of 40 where Leydig cells were diffusely distributed and their relative frequency per 0.1 mm² was calculated. The frequency of Leydig cells, thickness of tunica albuginea and cross sectional length s and breadths of seminiferous tubules were compared in between two age groups as well as in between left and right testes of the same group by using student’s t- test.

Source of Information: Bangl. J. Vet. Med. (2 008), 6(1): 67–74.

Article Link (Online Journal): http://dx.doi.org/10.3329/bjvm.v6i1.1341

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It was concluded that the thickness of tunica albuginea, the stratification of growing spermatogenic cells and cross sectional length and breadth of the seminiferous tubules of testes were higher in the bull of group B than group A and the number of Leydig cells were more in the testis of group A than group B and in between left and right testes, the thickness of tunica albuginea and cross sectional length and breadth of the seminiferous tubules were higher in the left testis but the number of Leydig cells was higher in right testis in both age groups.

Knowledge Management: Journal