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In Vitro Regeneration and Rapid Multiplication of Tuberose

M. R. Ali
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

M. H. Akand
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

A.F.M. Jamal Uddin
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

M. E. Hoque
Department of Biotechnology , Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

Homayra Huq
Department of Biotechnology , Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted at Tissue Culture Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh from July 2011 to March 2012 for In Vitro regeneration of tuberose. Experiment consisted ten combinations of different concentrated KIN and IAA as supplemented with MS medium. Early shoot (10.5 days), leaf (14.6 days) and root (16.0 days) initiation were found from MS+0.5 mg/L IAA +1.0 mg/L KIN. Maximum number of leaves (2.99/plantlet), length of leaf (5.48 cm) , number of roots (5.69/plantlet) and length of root (3.49 cm) were also found from MS+0.5 mg/L IAA +1.0 mg/L KIN.

Keywords: Tuberose, Regeneration, Multiplication

Location: Tissue Culture Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka

Start Date: 30-06-2011

End Date: 29-02-2012

Program Area: Variety and Species

Commodity: Tube rose

Objectives:

To develop protocols for in vitro regeneration and rapid multiplecation of tuberose

Methodology:

An experiment on in vitro regeneration of tuberose was conducted at Tissue Culture Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh from July 2011 to March 2012. Disease free bulbs of tuberose were collect ed from Bangladesh Agricultural Research Institute, Gazipur for explants in this experiment. MS medium were used with different hormonal supplements as culture medium for shoot initiation, root initiation and regeneration of tuberose. The culture tube containing medium was sterilized in an autoclave at temperature of 121°C for 20 minutes at l5 psi pressure. The medium was then cooled at room temperature before use. The lead of cabinet then was closed well and UV was switched on while turning off the air flow. The UV light of cabinet was left on for 30 minutes and the surface area is wiped with 70% ethanol. The temperature of culture room was maintained within 25±1 °C with the help of air conditioner with 16 hours photoperiod and 3000 lux light intensity by using white florescent lights. Buds were first sterilized with 70% (v/v) ethanol for few seconds after that surface sterilized by immersing in 0.1% HgCl₂ solution for 4-5 minutes and then was washed several times with sterilized distilled water. Then the bud was cut aseptically into small pieces with the help of sharp as eptic knife and the small segments of bud were used as explants. Three to five segments of explants were directly inoculated into each test tube containing 10 ml of MS medium supplemented with different callus induction hormonal combinations of IAA and KIN. Experiment consisted ten different hormonal combination viz. T₀: Simple MS, T₁: MS+0.5 mg/L IAA+0.5 mg/L KIN, T₂: MS+0.5 mg/L IAA +1.0 mg/L KIN, T₃: MS+0.5 mg/L IAA+1.5 mg/L KIN, T₄: MS+1.0 mg/L IAA+0.5 mg/L KIN, T₅: MS+1.0 mg/L IAA+1.0 mg/L KIN, T₆: MS+1.0 mg/L IAA+1.5 mg/L KIN, T₇: MS+1.5 mg/L IAA+0.5 mg/L KIN, T₈: MS+1.5 mg/L IAA+1.0 mg/L KIN and T₉: MS+1.5 mg/L IAA+1.5 mg/L KIN following Completely Randomized Design with five replications. Data were taken on days to shoot initiation, days to leaf initiation, days to root initiation, number of leaves/plantlet, length of leaf, number of roots/plantlet and length of root. Collected data on different parameters were analyzed using a MSTAT-C package computer program. The analysis of variance was performed and means were compared by Least Significant Difference (LSD) test at 5% level of significance.

Source of Information: INTERNATIONAL JOURNAL OF BUSINESS, SOCIAL AND SCIENTIFIC RESEARCH , Volume: 3, Issue: 1, Page: 35-38, 2015

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Tuberose could be successfully micro propagated with 0.5 mg/L IAA+1.0 mg/L KIN for rapid shoot and leaf initiation and proliferation.

Knowledge Management: Journal