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Screening of Chloramphenicol Residues in Chickens and Fish in Chittagong City of Bangladesh

M. A. Bakar
Bangladesh Council of Scientific and Industrial Research Laboratories, Chittagong, Bangladesh.

A. J. M. Morshed
Bangladesh Council of Scientific and Industrial Research Laboratories, Chittagong, Bangladesh.

F. Islam
Bangladesh Council of Scientific and Industrial Research Laboratories, Chittagong, Bangladesh.

R. Karim
Bangladesh Council of Scientific and Industrial Research, IFST, Dhaka, Bangladesh.

Abstract/ Executive Summary:

Chloramphenicol, an illegal antibiotic has been widely used in aquaculture and poultry as an antimicrobial agent which has been banned in many countries for its toxic side effects. A random screening of chloramphenicol residues in some poultry and fishes by Liquid Chromatography Tandem Mass Spectrometry (LCMS/MS) have been carried out. The determined concentrations of chloramphenicol in farmed and wild chickens and its liver, various types of farmed fishes were in the range of minimum value of 0.133 :g/ kg to the highest value of 0.515 :g/ kg. The lowest concentration of 0.133 :g/ kg was determined in Pungas fish and the highest of 0.515 :g/ kg in Rui fish. Minimum Required Performance Limit (MRPL) in food of animal origin like Meat, Eggs, Milk, Urine, Honey and Aquaculture products for Chloramphenicol is 0.3 :g/ kg. The results of eight samples out of thirteen have been exceeded the limit of MRPL. So it is a matter of great concern for us.

Keywords: Chicken, Fish, Chloramphenicol

Location: Chittagong City, Bangladesh

Start Date: 00-00-2003

End Date: 00-00-2013

Program Area: Animal Health and Management

Commodity: Chicken, Fish

Objectives:

To determine the assessment of chloramphenicol residue in chickens and fish by using Liquid Chromatography Tandem Mass Spectrometry (LCMSMS).

Methodology:

Samples were carefully handled to avoid contamination. Glassware was properly cleaned, and the reagents were of analytical grade. Deionized water was used throughout the study. The techniques for sample collection, extraction, standard preparation, analysis system for chloramphenicol have been briefly described below. Three varieties of chickens and six types of fishes were collected from local market in Chittagong City, Bangladesh in September, 2013. Five gram homogenized every sample was taken in a 50 ml ppt tube. Then the sample was homogenized using Ultraterex for 2 min after adding 5 ml deionized water. After that 10 ml ethyl acetate was added into the sample and mixed the solution by vortex mixture. Then the sample was centrifuged for 10 min at 5000 rpm. Then the ethyl acetate layer was transferred to 15 ml ppt tube and the sample was evaporated to dryness under a gentle stream of nitrogen on a heating at 55°C. Then the residue was dissolved in 1ml water. After adding 1ml hexane the solution mixed and centrifuged for 10 minutes at 3000 rpm. Then lower phase (Water layer) was collected and filtered it through a Phenex 0.45 Hm filter. Then it was transferred to an auto sampler vial for LC/MS/MS analysis. The stock solution of Chloramphenicol was prepared in Acetonirile at 1000 mg/l. The working standard solution of 0.1, 0.5, 1, 2.5, 5 and 10 Hg/l level for calibration curve were prepared by dilution in water on the day of analysis.

Source of Information: Bangl. J. Vet. Med. (2013), 11(2): 173-175, ISSN: 1729-7893 (Print) 2308-0922 (Online)

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

A random screening of chloramphenicol residues in some poultry and fishes by Liquid Chromatography Tandem Mass Spectrometry (LCMS/MS) have been carried out. The determined concentrations of chloramphenicol in farmed and wild chickens and its liver, various types of farmed fishes were in the range of minimum value of 0.133 :g/ kg to the highest value of 0.515 :g/ kg. The lowest concentration of 0.133 :g/ kg was determined in Pungas fish and the highest of 0.515 :g/ kg in Rui fish. Minimum Required Performance Limit (MRPL) in food of animal origin like Meat, Eggs, Milk, Urine, Honey and Aquaculture products for Chloramphenicol is 0.3 :g/ kg. The results of eight samples out of thirteen have been exceeded the limit of MRPL. So it is a matter of great concern for us.

Knowledge Management: Journal