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Growth Performance and Survival Rate of Macrobrachium Rosenbergii (de Man, 1979) Larvae Using Different Doses of Probiotics

Ahasan Habib
Department of Biology, Faculty of Science, Universiti Darussalam, Jalan Tunku Link, Gadong BE 1410, Brunei Darussalam

Nani Gopal Das
Institute of Marine Science & Fisheries, University of Chittagong, Chittagong 4331, Bangladesh

M. Belal Hossain
Department of Biology, Faculty of Science, Universiti Darussalam, Jalan Tunku Link, Gadong BE 1410, Brunei Darussalam; Department of Fiseries and Marine Science, Noakhali Science & Technology University, Bangladesh

Abstract/ Executive Summary:

The efficiency of probiotics (Ecomarine) in rearing of Macrobrachium rosenbergii larvae was evaluated in a commercial prawn hatchery for five weeks. Stage-1 (zero age) larvae (of length: 2 mm; weight: 0.12 mg) were stocked at the rate of 100 L^-1. The experiment determined the growth rate, survival rate of the larvae for the both treatment and control groups. Final average weight were found 8.39±3.28E-04 and 8.18±2.86E-04 mg and length were found 9.08±0.649 and 9.02±0.081 mm for treatment and control group respectively. Comparatively higher growth performance was observed in treatment than control. Post Larvae (PL) was first observed 20th days of culture in treatment tanks whereas PL in control tanks was found 24th days of culture. Survival rate was found 58 and 46% in treatment and control group respectively. There was significant (p<0.05) survival rate between two experiment groups. This study revealed that probiotics could be better in quality seed production of M. rosenbergii while significant changes were not noticed in the physic-chemical parameters i.e., water temperature, salinity, DO, pH, nitrate-NO₂, hardness and alkalinity observed in both the treatments.

Keywords: Probiotics, Growth performance, Survival rate, Macro bachium rogenbergii, Commercial hachery

Location: Department of Fiseries and Marine Science, Noakhali Science & Technology University, Bangladesh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Fish

Objectives:

The study was aimed to evaluate the effect of probiotics on larval growth and survival rate of Macrobrachium rosenbergii.

Methodology:

Experimental design: The experiment was conducted in a commercial hatchery. Same size plastic tanks (250 L) were used for larval rearing in which one treatment and a control group with three replicates each. The tanks were identified TA and CA. There was no alternative way to maintain optimum environment for larval growth and survival without daily water exchange. No water exchanged first two days of stocking. Water exchange rate was 70-80% (first week to fourth week) and 80% was last week that is until harvesting. This volume of water was exchanged at every afternoon. Probiotics were applied after water exchange. Whole duration of experiment was 35 days from larval rearing tank preparation to PL harvesting. Probiotics and application: Ecomarine probiotics was used for experiment. Its main component of five beneficial bacteria are B. subtilis, B. pumilus, B. amyloliquefaciens, B. megaterium, B. licheniformis. This probiotic was applied every day after exchange of water until PL harvesting. Doses were increased with increasing age of larvae. Probiotics was directly applied in the rearing water. Application doses of Ecomarine probiotic during larval rearing have been recorded. Feeds and feeding: Furthermore, live feed and formulated feed were given for larvae. Live feed (Artemia nauplii) was given at the rate of 5 n mL^-1 (first week) and then 4 n mL^-1 daily two times (morning and evening) until harvesting in all experiment groups. In addition, custard was given daily three times (early morning, morning and afternoon) from 9th day to harvesting at the following rate 1.4 g tank^-1 (second week), 2.14 g tank^-1 (third week) and finally 2.85 g tank^-1 until harvesting into all experiment groups. Water quality maintenance: Physico-chemical parameters were monitored every day. Siphoning and water exchange were performed daily. Water exchange was done 80% volume of every day. The aeration system in the backyard hatchery was performed with a compressor. Water quality was maintained by controlling the physio-chemical parameters of the rearing tanks. The following parameters such as water temperature, salinity, DO, pH, nitrate-NO₂ and hardness were recorded regular interval and maintained optimum condition. Among these nitrate-NO₂ and hardness were recorded every three days interval and remaining test were recorded daily by test kits and manually. Growth and weight analysis: Increment of weight was started from 8th day and continued for fifth week. So the increment of weight was recorded after the first week and continued for fifth week with a weekly interval. For measuring weight, 30 larvae (15 for each LRT) were sampled for Treatment and Control group, respectively. Larvae were weighted by electronic balance. The increment of length was recorded after the 1st week and continued for fifth weeks with a week interval. For measuring length, 30 larvae (15 for each LRT) were sampled for Treatment and control group, respectively. Larvae were measured by millimeter scale. Specific growth rate (SGR): The specific growth rate was determined from the following equation: SGR (%/day) = (logW_t - logW₀)/t , Where: W_t = Mean body weight (g) at time t, W₀ = Mean body weight (g) at time 0, and t = Time in day. Mean daily growth rate (g day^-1): Four separate mean daily growth rate values were recorded from 1st week to 5th week for every corresponding week of each Treatment and control and finally the overall mean daily growth rate (g day^-1) value was determined for the both Treatment and Control. Mean daily growth rate in terms of length and weight were determined from a simple mathematical equation: Mean daily growth rate (g/day) = (W_t - W₀)/t , and Mean daily growth rate (mm/day), where, W_t = Mean weight (g) time t, W₀ = Mean weight (g) at time 0, L_t = Mean length (mm) at time t, L₀ = Mean length (mm) at time 0, t = times in day. Survival rate (%): Survival rate was recorded after the 1st week and continued for next five weeks with a weekly interval. Survival rate was calculated by using the following equation : Survival rate (%) = Total Population/Total no of larvae stocked x 100. Statistical analysis: Recorded data were analyzed using Microsoft Excel 2007 software. Survival rate between initial number of fries and alive number of fries after a week wasanalysed using regression analysis and level of significance was p<0.05 (95% confidence level).

Source of Information: Pakistan Journal of Biological Sciences, 17: 920-924; ISSN 1028 8880

Article Link (Online Journal): DOI: 10.3923/pjbs.2014.920.924; URL: http://scialert.net/abstract/?doi=pjbs.2014.920.924

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It was observed that by using of probiotics in hatchery, production volume was increased by about 12% rather than as usual (without any additives) production practice in hatchery. Furthermore, probiotics is a welcome addition to protection of diseases as well as reduce various gases in rearing tanks. Finally, the success of aquaculture in future might be expanded with the success of probiotics applications.

Knowledge Management: Journal